BD FACSymphony S6 cell sorter

BD FACSymphony S6 细胞分选仪

• 批准号: 10425932
• 负责人: DAVID A BRAFMAN
• 金额: $ 59.93万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425932
• 关键词: Arizona; Biological; Cell Separation; Cells; Color; Core Facility; Faculty; Flow Cytometry; Funding; Laboratories; Laboratory Research; Lasers; Liquid substance; Population; Research; Research Personnel; Resources; Safety; Scientist; Sorting - Cell Movement; System; Technology; United States National Institutes of Health; Universities; Viral; flexibility; instrument; sequencing platform; single-cell RNA sequencing

PROJECT SUMMARY ABSTRACT Over the last 15 years, the Arizona State University (ASU) Flow Cytometry Core Facility (FCCF) has been serving the research needs of a diverse set of research groups. Currently, the ASU FCCF regularly serves the research needs of over 40 research laboratories across 6 academic units. Critically, this facility serves as the only shared core laboratory that provides flow cytometry sorting capabilities to the researches on the ASU Tempe campus. The current flow cytometer in the facility, the BD FACSAria I, was purchased in 2005 and has outgrown both the cell sorting capacity and technology needs required by new faculty as well as current researchers who have enhanced needs. To that end, this proposal is requesting funds to acquire a BD FACSymphony S6 cell sorter configured with a 5 laser, 30-color system. This enhanced system will provide users additional sensitivity and flexibility when compared to the current 3 laser, 9-color FACSAria I instrument present in the FCCF. In addition, the upgraded fluidics (which has been a limiting feature of the current FACSAria I system) of the BD FACSymphony S6 will allow for greater throughput and survival of rare and sensitive cell populations which are typical of the research groups that use the ASU FCCF. Moreover, the 6-way and single cell sorting capacities of the BD FACSymphony S6 are compatible with the downstream single-cell RNA-sequencing platforms that are becoming increasingly employed by ASU FCCF users. Finally, the seamless integration of the BD FACSymphony S6 with a Baker biological safety cabinet will allow for the sorting of virally transfected cell populations which is not easily achievable with the current FACSAria I system present in the FCCF. Overall, acquisition of this new instrument will provide a resource currently not available to ASU scientists as well enable and enhance the research of NIH-funded investigators.

项目摘要摘要 在过去的15年中，亚利桑那州立大学（ASU）流式细胞仪核心设施（FCCF）一直在服务 各种各样的研究小组的研究需求。目前，ASU FCCF定期为研究服务 在6个学术单位的40多个研究实验室的需求。至关重要的是，该设施是唯一共享的 为ASU Tempe校园的研究提供流式细胞术分类功能的核心实验室。 当前设施中的流式细胞仪BD FACSARIA I于2005年购买，两者都超过了 新教师以及现有的研究人员所需的细胞分类能力和技术需求 增强需求。为此，该提案要求资金购买BD FACSYMPHONY S6 Cell Sorter 配置为5激光，30色系统。这种增强的系统将为用户提供额外的敏感性和 与当前的3激光，9色Facsaria I仪器相比，FCCF中的灵活性。此外， BD的升级流体（这是当前FACSARIA I系统的限制功能） Facsymphony S6将允许更大的吞吐量和稀有细胞群体的吞吐量和存活率 使用ASU FCCF的研究小组的典型代表。此外，6向和单细胞分类容量 BD FACSymphony S6与下游的单细胞RNA序列平台兼容 被ASU FCCF用户越来越多地使用。最后，BD的无缝集成 带有面包师生物安全柜的Facsymphony S6将允许分类病毒转染的单元 FCCF中存在的当前FACSARIA I系统不容易实现的种群。全面的， 获取该新工具将为ASU科学家提供当前无法获得的资源。 并增强了NIH资助的研究人员的研究。