BD FACSymphony S6 cell sorter

BD FACSymphony S6 细胞分选仪

• 批准号: 10425932
• 负责人: DAVID A BRAFMAN
• 金额: $ 59.93万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425932
• 关键词: Arizona; Biological; Cell Separation; Cells; Color; Core Facility; Faculty; Flow Cytometry; Funding; Laboratories; Laboratory Research; Lasers; Liquid substance; Population; Research; Research Personnel; Resources; Safety; Scientist; Sorting - Cell Movement; System; Technology; United States National Institutes of Health; Universities; Viral; flexibility; instrument; sequencing platform; single-cell RNA sequencing

PROJECT SUMMARY ABSTRACT Over the last 15 years, the Arizona State University (ASU) Flow Cytometry Core Facility (FCCF) has been serving the research needs of a diverse set of research groups. Currently, the ASU FCCF regularly serves the research needs of over 40 research laboratories across 6 academic units. Critically, this facility serves as the only shared core laboratory that provides flow cytometry sorting capabilities to the researches on the ASU Tempe campus. The current flow cytometer in the facility, the BD FACSAria I, was purchased in 2005 and has outgrown both the cell sorting capacity and technology needs required by new faculty as well as current researchers who have enhanced needs. To that end, this proposal is requesting funds to acquire a BD FACSymphony S6 cell sorter configured with a 5 laser, 30-color system. This enhanced system will provide users additional sensitivity and flexibility when compared to the current 3 laser, 9-color FACSAria I instrument present in the FCCF. In addition, the upgraded fluidics (which has been a limiting feature of the current FACSAria I system) of the BD FACSymphony S6 will allow for greater throughput and survival of rare and sensitive cell populations which are typical of the research groups that use the ASU FCCF. Moreover, the 6-way and single cell sorting capacities of the BD FACSymphony S6 are compatible with the downstream single-cell RNA-sequencing platforms that are becoming increasingly employed by ASU FCCF users. Finally, the seamless integration of the BD FACSymphony S6 with a Baker biological safety cabinet will allow for the sorting of virally transfected cell populations which is not easily achievable with the current FACSAria I system present in the FCCF. Overall, acquisition of this new instrument will provide a resource currently not available to ASU scientists as well enable and enhance the research of NIH-funded investigators.

项目摘要 在过去的15年里，亚利桑那州立大学（ASU）流式细胞术核心设施（FCCF）一直在服务于 一组不同的研究小组的研究需求。目前，ASU FCCF定期为研究提供服务 6个学术单位的40多个研究实验室的需求。关键是，这个设施是唯一一个 核心实验室，为亚利桑那州立大学滕佩校区的研究提供流式细胞仪分选能力。 该设施中目前的流式细胞仪BD FACSAria I于2005年购买， 细胞分选能力和新教师以及现有研究人员所需的技术需求， 增强的需求。为此，该提案要求提供资金，以购买BD FACSymphony S6细胞分选机 配置5激光30色系统。这一增强的系统将为用户提供额外的灵敏度， 与目前FCCF中的3激光、9色FACSAria I仪器相比，此外，本发明还提供了一种方法， BD的升级射流技术（这是当前FACSAria I系统的一个限制性特征） FACSymphony S6将允许更大的通量和稀有和敏感细胞群的存活， 这是使用ASU FCCF的典型研究小组。此外，6路和单细胞分选能力， BD FACSymphony S6与下游单细胞RNA测序平台兼容， 越来越多地被ASU FCCF用户使用。最后，BD的无缝集成 配备Baker生物安全柜的FACSymphony S6将允许对病毒转染细胞进行分选 这是目前FCCF中的FACSAria I系统不容易实现的。总的来说， 购买这台新仪器将为亚利桑那州立大学的科学家提供目前无法获得的资源， 并加强NIH资助的研究人员的研究。