Linear Ion Trap - Quadrupole LC-MS System

线性离子阱 - 四极杆 LC-MS 系统

• 批准号: 10425494
• 负责人: Krishnarao Maddipati
• 金额: $ 59.22万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425494
• 关键词: 10 year old; Aging; Area; Biological Markers; Cardiovascular Diseases; Chemistry; Chronic Disease; Core Facility; Diabetes Mellitus; Disease; Evolution; Funding; Inflammation; Inflammation Mediators; Institutes; Ions; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Mediator of activation protein; Neurodegenerative Disorders; Patients; Pharmacology; Physiological; Proteomics; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Scanning; System; Time; United States National Institutes of Health; Universities; faculty research; instrument; instrumentation; lipid mediator; lipidome; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; metabolomics; novel; small molecule; ultra high pressure

Project Summary/Abstract This proposal is for a new sensitive and wider mass range LC/MS system, both to enhance the existing capabilities of our Lipidomics Core Facility and to provide a backup for the aging mass spectrometry resource to NIH funded researchers at Wayne State University. Currently mass spectrometry resources at Wayne State University are located at the Lumigen Instrumentation Center in Chemistry, the Pharmacology Core of the Karmanos Cancer Institute (KCI), the Proteomics Core, and the Lipidomics Core. However, none of the available instruments are sensitive enough to accurately quantify lipid mediators such as Specialized Pro-resolution Mediators (SPMs) that are physiologically active at picomolar concentrations. Analyzing such biomolecules in limited sample volumes that are collected from patients require sensitivity in the low femtogram range. One of the major areas of the participating faculty's research involves identification and/or quantitation of such lipid mediators of inflammation and resolution of inflammation (e.g. SPMs) in diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders. Lipidomics Core Facility currently utilizes a 10-year-old LC-MS system with limited mass range (<1200 Da), insufficient to analyze a significant part of the lipidome, and sensitivity that is about 10-times lower than required for the analysis of important lipid mediators. The proposed acquisition of a linear ion-trap triple quadrupole mass spectrometry system with its high sensitivity, fast scanning, more than 5 orders of magnitude dynamic range of quantitation along with an ultra-high pressure liquid chromatographic system fills a great void in our analytical needs. Given the focus of the users on the identification of novel small molecule biomarkers of inflammation and related chronic diseases such as cancer and diabetes, this mass spectrometer is urgent and vital for our research projects. Additionally, this instrument will catalyze the evolution of our Lipidomics core facility into a full-fledged metabolomics core at Wayne State University.

项目总结/摘要 该建议是一种新的灵敏和更宽的质量范围的LC/MS系统， 增强我们脂质组学核心设施的现有能力，并提供备份 向美国国家卫生研究院资助的韦恩州立大学的研究人员提供老化质谱资源 大学目前，韦恩州立大学的质谱资源位于 在Lumigen化学仪器中心， Karmanos癌症研究所（KCI），蛋白质组学核心和脂质组学核心。 然而，现有的仪器都不够灵敏，无法准确量化 脂质介质，如专门的促消退介质（SPMs）， 在皮摩尔浓度下具有生理活性。在有限的时间内分析这些生物分子 从患者体内采集的样本量要求在低毫微微克下具有灵敏度 范围参与研究的教师的主要领域之一涉及识别 和/或定量这种炎症的脂质介质和炎症的消退 (e.g. SPM）在癌症、糖尿病、心血管和神经退行性疾病中的应用 紊乱Lipidomics Core Facility目前使用10年历史的LC-MS系统， 有限的质量范围（<1200 Da），不足以分析脂质组的重要部分， 并且灵敏度比分析重要的脂质所需的低约10倍 调解员线性离子阱三重四极质量的获得 光谱分析系统具有灵敏度高、扫描速度快、光谱分辨率可达5阶以上等优点， 用超高压液体沿着定量的幅度动态范围 色谱系统填补了我们分析需求的巨大空白。考虑到 使用者对炎症和相关疾病的新型小分子生物标志物的鉴定 癌症和糖尿病等慢性疾病，这种质谱仪是紧迫和至关重要的 我们的研究项目。此外，这一工具将促进我们的 将脂质组学核心设施改造成韦恩州立大学成熟的代谢组学核心。