Linear Ion Trap - Quadrupole LC-MS System

线性离子阱 - 四极杆 LC-MS 系统

• 批准号: 10425494
• 负责人: Krishnarao Maddipati
• 金额: $ 59.22万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425494
• 关键词: 10 year old; Aging; Area; Biological Markers; Cardiovascular Diseases; Chemistry; Chronic Disease; Core Facility; Diabetes Mellitus; Disease; Evolution; Funding; Inflammation; Inflammation Mediators; Institutes; Ions; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Mediator of activation protein; Neurodegenerative Disorders; Patients; Pharmacology; Physiological; Proteomics; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Scanning; System; Time; United States National Institutes of Health; Universities; faculty research; instrument; instrumentation; lipid mediator; lipidome; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; metabolomics; novel; small molecule; ultra high pressure

Project Summary/Abstract This proposal is for a new sensitive and wider mass range LC/MS system, both to enhance the existing capabilities of our Lipidomics Core Facility and to provide a backup for the aging mass spectrometry resource to NIH funded researchers at Wayne State University. Currently mass spectrometry resources at Wayne State University are located at the Lumigen Instrumentation Center in Chemistry, the Pharmacology Core of the Karmanos Cancer Institute (KCI), the Proteomics Core, and the Lipidomics Core. However, none of the available instruments are sensitive enough to accurately quantify lipid mediators such as Specialized Pro-resolution Mediators (SPMs) that are physiologically active at picomolar concentrations. Analyzing such biomolecules in limited sample volumes that are collected from patients require sensitivity in the low femtogram range. One of the major areas of the participating faculty's research involves identification and/or quantitation of such lipid mediators of inflammation and resolution of inflammation (e.g. SPMs) in diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders. Lipidomics Core Facility currently utilizes a 10-year-old LC-MS system with limited mass range (<1200 Da), insufficient to analyze a significant part of the lipidome, and sensitivity that is about 10-times lower than required for the analysis of important lipid mediators. The proposed acquisition of a linear ion-trap triple quadrupole mass spectrometry system with its high sensitivity, fast scanning, more than 5 orders of magnitude dynamic range of quantitation along with an ultra-high pressure liquid chromatographic system fills a great void in our analytical needs. Given the focus of the users on the identification of novel small molecule biomarkers of inflammation and related chronic diseases such as cancer and diabetes, this mass spectrometer is urgent and vital for our research projects. Additionally, this instrument will catalyze the evolution of our Lipidomics core facility into a full-fledged metabolomics core at Wayne State University.

项目概要/摘要 该提案针对的是一种新的灵敏且质量范围更宽的 LC/MS 系统， 增强我们脂质组学核心设施的现有能力并提供备份 向 NIH 资助的韦恩州立大学研究人员提供老化质谱资源 大学。目前韦恩州立大学的质谱资源位于 在 Lumigen 化学仪器中心，该中心的药理学核心 卡马诺斯癌症研究所 (KCI)、蛋白质组学核心和脂质组学核心。 然而，没有任何可用的仪器足够灵敏，无法准确量化 脂质介质，例如专门的促分辨率介质 (SPM) 在皮摩尔浓度下具有生理活性。在有限的范围内分析此类生物分子 从患者收集的样本量需要低飞克级的灵敏度 范围。参与教师研究的主要领域之一涉及识别 和/或此类炎症脂质介质的定量和炎症的消退 （例如 SPM）在癌症、糖尿病、心血管和神经退行性疾病等疾病中的应用 失调。脂质组学核心设施目前使用已有 10 年历史的 LC-MS 系统 质量范围有限（<1200 Da），不足以分析脂质组的重要部分， 灵敏度比重要脂质分析所需的灵敏度低约 10 倍 调解员。线性离子阱三重四极杆质量的拟议采集 光谱分析系统灵敏度高，扫描速度快，超过5个量级 超高压液体定量的动态范围 色谱系统填补了我们分析需求的巨大空白。鉴于重点 用户识别炎症及相关的新型小分子生物标志物 癌症、糖尿病等慢性疾病，质谱仪紧急且至关重要 用于我们的研究项目。此外，该工具将促进我们的发展 将脂质组学核心设施转变为韦恩州立大学成熟的代谢组学核心。