Linear Ion Trap - Quadrupole LC-MS System

线性离子阱 - 四极杆 LC-MS 系统

• 批准号: 10425494
• 负责人: Krishnarao Maddipati
• 金额: $ 59.22万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425494
• 关键词: 10 year old; Aging; Area; Biological Markers; Cardiovascular Diseases; Chemistry; Chronic Disease; Core Facility; Diabetes Mellitus; Disease; Evolution; Funding; Inflammation; Inflammation Mediators; Institutes; Ions; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Mediator of activation protein; Neurodegenerative Disorders; Patients; Pharmacology; Physiological; Proteomics; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Scanning; System; Time; United States National Institutes of Health; Universities; faculty research; instrument; instrumentation; lipid mediator; lipidome; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; metabolomics; novel; small molecule; ultra high pressure

Project Summary/Abstract This proposal is for a new sensitive and wider mass range LC/MS system, both to enhance the existing capabilities of our Lipidomics Core Facility and to provide a backup for the aging mass spectrometry resource to NIH funded researchers at Wayne State University. Currently mass spectrometry resources at Wayne State University are located at the Lumigen Instrumentation Center in Chemistry, the Pharmacology Core of the Karmanos Cancer Institute (KCI), the Proteomics Core, and the Lipidomics Core. However, none of the available instruments are sensitive enough to accurately quantify lipid mediators such as Specialized Pro-resolution Mediators (SPMs) that are physiologically active at picomolar concentrations. Analyzing such biomolecules in limited sample volumes that are collected from patients require sensitivity in the low femtogram range. One of the major areas of the participating faculty's research involves identification and/or quantitation of such lipid mediators of inflammation and resolution of inflammation (e.g. SPMs) in diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders. Lipidomics Core Facility currently utilizes a 10-year-old LC-MS system with limited mass range (<1200 Da), insufficient to analyze a significant part of the lipidome, and sensitivity that is about 10-times lower than required for the analysis of important lipid mediators. The proposed acquisition of a linear ion-trap triple quadrupole mass spectrometry system with its high sensitivity, fast scanning, more than 5 orders of magnitude dynamic range of quantitation along with an ultra-high pressure liquid chromatographic system fills a great void in our analytical needs. Given the focus of the users on the identification of novel small molecule biomarkers of inflammation and related chronic diseases such as cancer and diabetes, this mass spectrometer is urgent and vital for our research projects. Additionally, this instrument will catalyze the evolution of our Lipidomics core facility into a full-fledged metabolomics core at Wayne State University.

项目摘要/摘要 该建议是针对新的敏感质量范围LC/MS系统的，均为 增强我们脂肪组学核心设施的现有功能并提供备份 对于NIH资助的Wayne State的NIH资助的研究人员的老化质谱资源 大学。目前，韦恩州立大学的质谱资源位于 在化学的Lumigen仪器中心，是药理学的核心 Karmanos癌症研究所（KCI），蛋白质组学核心和脂肪组学核心。 但是，没有任何可用仪器足够敏感以准确量化 脂质介质（例如专门的促分辨率介体（SPM）） 生理活性在皮摩尔浓度下。在有限的情况下分析此类生物分子 从患者中收集的样品体积需要在低操作图中灵敏度 范围。参与教师的研究的主要领域之一是识别 和/或定量这种炎症的脂质介质和炎症的分辨率 （例如，SPMS）在癌症，糖尿病，心血管和神经退行性等疾病中 疾病。脂肪组学核心设施目前使用了10年历史的LC-MS系统 有限的质量范围（<1200 da），不足以分析脂质组的重要部分， 敏感性比分析重要脂质所需的灵敏度低约10倍 调解人。拟议的线性离子陷阱三倍四极块质量 光谱系统具有高灵敏度，快速扫描，超过5个顺序 大小的动态定量范围以及超高压液体 色谱系统填补了我们的分析需求。考虑到重点 用户鉴定了新型小分子生物标志物的炎症和相关的用户 慢性疾病，例如癌症和糖尿病，这种质谱仪是紧迫而重要的 对于我们的研究项目。此外，该仪器将催化我们的演变 脂肪组学核心设施成为韦恩州立大学成熟的代谢组学核心。