Live Cell Imaging System for Biomechanics and Mechanobiology Research

用于生物力学和力学生物学研究的活细胞成像系统

• 批准号: 10431500
• 负责人: Gunes Uzer
• 金额: $ 23.32万
• 依托单位: BOISE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431500
• 关键词: 3-Dimensional; Arts; Basic Science; Biology; Biomechanics; Biomedical Engineering; Biomedical Research; Bioreactors; Cells; Centers of Research Excellence; Coupled; Cues; Custom; Disease; Doctor of Philosophy; Engineering; Equipment; Faculty; Funding; Goals; Growth; Health; Health Sciences; Idaho; Institution; Investigation; Investments; Link; Mechanics; Microscope; Microscopy; Molecular; Musculoskeletal; Musculoskeletal System; Orthopedics; Physiological; Pongidae; Positioning Attribute; Process; Research; Research Personnel; Science; System; Tissues; United States National Institutes of Health; Universities; clinical application; college; extracellular; imaging system; innovation; instrument; live cell imaging; programs; rapid growth

Project Summary The goal of this proposal is to acquire a Zeiss Axio Observer 7 Live Cell Imaging system which will be coupled with available custom-made and commercial bioreactor systems that will enable biomedical research focused on deformation microscopy, mechano-adaptation and cell tracking approaches for researchers at Boise State as well as neighboring and collaborator institutions. Led by NIH initiatives Center of Biomedical Research Excellence (COBRE) at Matrix Biology and Idaho Network of Biomedical Research Excellence (INBRE) there was a rapid growth of biomedical research focused at biomechanics and mechanobiology in Boise State. These efforts are further supported by both new PhD programs like Biomedical Engineering and by established PhD programs such Materials Engineering and Biomolecular Sciences that seen increased Bioengineering focus over the years. Despite this growth, there is only one confocal microscope to serve all the researchers in Boise State. This not only bottlenecks the use of confocal system which is better suited for 3D applications, but also limits mechanistic approaches to study how extracellular cues regulate function of living cells. The Axio Observer 7 with mechanobiology apabilities is a critical instrument for a diverse and growing number of biomedical researchers at Boise State University. These researchers include biomedical engineers, biomechanists, and biologists from three colleges: College of Engineering, College of Health Sciences, and College of Arts and Sciences. A unifying theme of this group’s research is the investigation of mechanically active musculoskeletal systems and materials in normal, diseased, and treated states. With strategic investments by Boise State University, this group has rapidly grown in the last 10 years (7 new faculty, 5 new labs), creating a critical mass of complementary expertise that is positioned to collaborate on transformative and synergistic orthopaedic and musculoskeletal research. Yet achieving this potential requires the acquisition of state-of-the-art equipment to investigate the physiological and pathophysiological processes from molecular to tissue scales. Our ability to link this structural hierarchy is currently limited by the absence of a system that can apply physiologically relevant forces with a capability to observe molecular and mechanical consequences. As a result, biomedical faculty at Boise State University are poorly equipped to transition from discoveries in basic science to innovations with direct clinical application related to musculoskeletal health. Therefore there is a clear benefit of this live-cell imaging and mechanosignaling capabilities offered by this microscope system. The acquisition of the Axio Observer 7 with mechanobiology capabilities will correct this deficiency and will not only benefit NIH funded current project but also spur the initiation of many impactful projects with high funding potential.

项目摘要 该提案的目的是获取Zeiss Axio观察者7 Live Cell成像系统，该系统将耦合 使用可用的定制和商业生物反应器系统，可以以生物医学研究为重点 在变形显微镜下，博伊西州研究人员的机械适应和细胞跟踪方法 以及邻近和合作机构。由NIH生物医学研究中心领导 Matrix Biology和Idaho生物医学研究卓越网络（INBRE）的卓越（COBRE） 是博伊西州生物力学和机械生物学的生物医学研究的快速增长。这些 诸如生物医学工程和既定博士学位的新博士学位课程都进一步支持了努力 计划材料工程和生物分子科学，使生物工程的重点增加了 年。尽管增长了这种增长，但只有一个共聚焦显微镜可以为博伊西州的所有研究人员提供服务。 这不仅可以瓶颈使用共聚焦系统，该系统更适合3D应用程序，而且还限制 研究细胞外提示如何调节活细胞功能的机械方法。 Axio观察者7 通过机制，关键的工具是多样性和越来越多的生物医学 博伊西州立大学的研究人员。这些研究人员包括生物医学工程师，生物力学和 来自三所大学的生物学家：工程学院，卫生科学学院和艺术学院， 科学。该小组研究的一个统一主题是机械活跃的肌肉骨骼的投资 正常，解散和处理状态的系统和材料。博伊西州的战略投资 大学，该小组在过去的10年中迅速发展（7个新教师，5个新实验室），创造了一个临界质量 完善的专业知识，可以在变革性和协同骨科和协同性的骨科和 肌肉骨骼研究。然而，实现这一潜力需要获取最先进的设备才能 研究从分子到组织量表的物理和病理生理过程。我们链接的能力 该结构层次结构目前受到无法应用物理相关的系统的限制 有能力观察分子和机械后果的力。结果，生物医学教师 博伊西州立大学从基础科学的发现过渡到与 与肌肉骨骼健康有关的直接临床应用。因此，这个活细胞有明显的好处 该显微镜系统提供的成像和机械信号功能。 Axio的获取 具有机械生物学能力的观察者7将纠正这种缺陷，不仅会使NIH资助。 当前的项目，但也促进了许多具有高资金潜力的有影响力项目的倡议。