Live Cell Imaging System for Biomechanics and Mechanobiology Research

用于生物力学和力学生物学研究的活细胞成像系统

• 批准号: 10431500
• 负责人: Gunes Uzer
• 金额: $ 23.32万
• 依托单位: BOISE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431500
• 关键词: 3-Dimensional; Arts; Basic Science; Biology; Biomechanics; Biomedical Engineering; Biomedical Research; Bioreactors; Cells; Centers of Research Excellence; Coupled; Cues; Custom; Disease; Doctor of Philosophy; Engineering; Equipment; Faculty; Funding; Goals; Growth; Health; Health Sciences; Idaho; Institution; Investigation; Investments; Link; Mechanics; Microscope; Microscopy; Molecular; Musculoskeletal; Musculoskeletal System; Orthopedics; Physiological; Pongidae; Positioning Attribute; Process; Research; Research Personnel; Science; System; Tissues; United States National Institutes of Health; Universities; clinical application; college; extracellular; imaging system; innovation; instrument; live cell imaging; programs; rapid growth

Project Summary The goal of this proposal is to acquire a Zeiss Axio Observer 7 Live Cell Imaging system which will be coupled with available custom-made and commercial bioreactor systems that will enable biomedical research focused on deformation microscopy, mechano-adaptation and cell tracking approaches for researchers at Boise State as well as neighboring and collaborator institutions. Led by NIH initiatives Center of Biomedical Research Excellence (COBRE) at Matrix Biology and Idaho Network of Biomedical Research Excellence (INBRE) there was a rapid growth of biomedical research focused at biomechanics and mechanobiology in Boise State. These efforts are further supported by both new PhD programs like Biomedical Engineering and by established PhD programs such Materials Engineering and Biomolecular Sciences that seen increased Bioengineering focus over the years. Despite this growth, there is only one confocal microscope to serve all the researchers in Boise State. This not only bottlenecks the use of confocal system which is better suited for 3D applications, but also limits mechanistic approaches to study how extracellular cues regulate function of living cells. The Axio Observer 7 with mechanobiology apabilities is a critical instrument for a diverse and growing number of biomedical researchers at Boise State University. These researchers include biomedical engineers, biomechanists, and biologists from three colleges: College of Engineering, College of Health Sciences, and College of Arts and Sciences. A unifying theme of this group’s research is the investigation of mechanically active musculoskeletal systems and materials in normal, diseased, and treated states. With strategic investments by Boise State University, this group has rapidly grown in the last 10 years (7 new faculty, 5 new labs), creating a critical mass of complementary expertise that is positioned to collaborate on transformative and synergistic orthopaedic and musculoskeletal research. Yet achieving this potential requires the acquisition of state-of-the-art equipment to investigate the physiological and pathophysiological processes from molecular to tissue scales. Our ability to link this structural hierarchy is currently limited by the absence of a system that can apply physiologically relevant forces with a capability to observe molecular and mechanical consequences. As a result, biomedical faculty at Boise State University are poorly equipped to transition from discoveries in basic science to innovations with direct clinical application related to musculoskeletal health. Therefore there is a clear benefit of this live-cell imaging and mechanosignaling capabilities offered by this microscope system. The acquisition of the Axio Observer 7 with mechanobiology capabilities will correct this deficiency and will not only benefit NIH funded current project but also spur the initiation of many impactful projects with high funding potential.

项目概要 该提案的目标是购买蔡司 Axio Observer 7 活细胞成像系统，该系统将与 拥有可用的定制和商业生物反应器系统，使生物医学研究能够集中 为博伊西州立大学的研究人员提供变形显微镜、机械适应和细胞跟踪方法 以及邻近和合作机构。由 NIH 生物医学研究中心倡议领导 Matrix Biology 的卓越 (COBRE) 和爱达荷州生物医学研究卓越网络 (INBRE) 是博伊西州立大学专注于生物力学和机械生物学的生物医学研究的快速发展。这些 生物医学工程等新的博士课程和已建立的博士课程进一步支持了这些努力 诸如材料工程和生物分子科学等项目，生物工程的关注度越来越高 这些年。尽管有这种增长，但只有一台共焦显微镜可以为博伊西州立大学的所有研究人员提供服务。 这不仅限制了更适合 3D 应用的共焦系统的使用，而且限制了 研究细胞外信号如何调节活细胞功能的机械方法。 Axio 观察者 7 具有机械生物学能力，是多样化且不断增长的生物医学研究的重要工具 博伊西州立大学的研究人员。这些研究人员包括生物医学工程师、生物力学学家和 来自三个学院的生物学家：工程学院、健康科学学院、艺术学院 科学。该小组研究的一个统一主题是研究机械活动的肌肉骨骼 正常、患病和治疗状态下的系统和材料。博伊西州立大学战略投资 大学，这个群体在过去 10 年里迅速发展（7 个新教师，5 个新实验室），形成了一个临界群体 具有互补的专业知识，能够在变革性和协同性骨科领域进行合作 肌肉骨骼研究。然而，实现这一潜力需要购买最先进的设备 研究从分子到组织尺度的生理和病理生理过程。我们的链接能力 这种结构层次目前由于缺乏可以应用生理相关的系统而受到限制 具有观察分子和机械后果的能力的力。因此，生物医学系 博伊西州立大学缺乏从基础科学发现到创新的过渡 与肌肉骨骼健康相关的直接临床应用。因此，这种活细胞有一个明显的好处 该显微镜系统提供的成像和机械信号功能。收购Axio 具有机械生物学能力的 Observer 7 将纠正这一缺陷，不仅使 NIH 资助的项目受益 目前的项目还刺激了许多具有高融资潜力的有影响力的项目的启动。