Acquisition of next-generation Orbitrap Eclipse Tribrid Mass Spectrometer system

采购下一代 Orbitrap Eclipse Tribrid 质谱仪系统

• 批准号: 10431262
• 负责人: Jia Fan
• 金额: $ 137.31万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431262
• 关键词: Address; Area; Biomedical Research; Cells; Characteristics; Data; Dissociation; Electron Transport; Faculty; Funding; Generations; Grant; Label; Mass Spectrum Analysis; Methods; Minor; Modification; Performance; Proteins; Proteomics; Public Health; Research; Research Personnel; Research Priority; Research Project Grants; Resolution; Scanning; Speed; Surveys; System; Time; Translations; United States National Institutes of Health; Universities; base; biomarker development; clinical translation; data acquisition; design; disease diagnosis; improved; instrument; mass analyzer; mass spectrometer; meetings; member; nano-liquid chromatography; next generation; targeted treatment

Project Summary Many NIH-funded faculties at Tulane University require state-of-art LC-MS/MS to conduct biomedical research projects intended for clinical translation. A survey of these users revealed several features that were missing from instruments available to Tulane faculty members, while our research on available instruments found one set of instruments that could address these unmet needs: Orbitrap Eclipse Tribrid mass analyzer systems. The proposed ultra-high-sensitive Orbitrap Eclipse Tribrid LC-MS/MS system possesses several unique features that are essential for our research but are not available on our existing platform, such as single-cell proteomics, multistage mass spectrometry (MSn) capability, increased precursor dissociation options, and Real-time Search Synchronous Precursor Selection (SPS MS3) Tandem Mass Tag (TMT) data acquisition mode. We have confirmed that the Orbitrap Eclipse Tribrid mass spectrometer with nano liquid chromatography, UltiMate 3000 RSLCnano (U3000 LC) system meets or exceeds the needs of our user base and offers superior performance relative to other similar available LC-MS/MS systems. The proposed instrument will support the discovery of new biomarkers and the development of new methods of significant biomedical relevance, which we expect will lead to breakthroughs in disease diagnosis, mechanism studies, and targeted therapy. The proposed Orbitrap Eclipse Tribrid mass spectrometry with U3000 nanoLC system represents the next generation of LC-MS/MS system. The instrument provides higher resolution and faster scan speeds, and can perform parallel acquisition to maximize the duty cycle. Our demonstration project with Thermo Scientific showed that the real-time search SPS MS3 method improved identification rates by 30% with higher accuracy and less interference than the MS2 method by Q Exactive HF-x, Tulane’s existing mass spectrometry system. The identification rates via the Data-dependent acquisition (DDA) method increased by 76% using Eclipse with FAIMS Pro interface at the protein level, and increased by 30% using the Data-independent acquisition (DIA)- based label-free method. The requested electron-transfer dissociation method could better support post- translation modification analysis with more confident identification. The new generation tribrid MS gives added experimental throughput while providing depth, coverage, and single-cell proteomics capabilities. These performance characteristics will be highly useful to the large base of Tulane researchers using advanced LC- MS/MS analyses for biomedical research. We have identified an instrument user base of 32 faculty members (12 major users and 20 minor users) funded by NIH or other federal grants. They have requested the features provided by the proposed instrument to conduct their studies, demonstrating the significant need for the requested system. Meeting this need, therefore, is expected to advance multiple high-impact biomedical research projects designed to promote public health across a wide array of subject areas and NIH research priorities.

项目摘要 杜兰大学的许多NIH资助的学院要求最先进的LC-MS/MS进行生物医学研究 用于临床翻译的项目。对这些用户的调查揭示了几个缺少的功能 从可用的工具到图兰教师，而我们对可用工具的研究发现了一种 可以满足这些未满足需求的一组工具：Orbitrap Eclipse Tribrid质量分析仪系统。这 拟议的超高敏感的轨道轨道日食三元型LC-MS/MS系统潜力有几个独特的功能 对于我们的研究至关重要，但在我们现有平台上不可用，例如单细胞蛋白质组学， 多阶段质谱（MSN）功能，前体解离选项增加和实时搜索 同步前体选择（SPS MS3）串联质量标签（TMT）数据采集模式。 我们已经证实，甲型液态色谱法的Orbitrap Eclipse三元质谱仪， Ultimate 3000 RSLCNANO（U3000 LC）系统满足或超出了我们的用户群的需求，并提供了优越的 性能相对于其他类似的LC-MS/MS系统。提议的仪器将支持 发现新的生物标志物以及开发具有重要生物医学相关性的新方法， 我们预计将导致疾病诊断，机理研究和靶向治疗方面的突破。 拟议的Orbitrap Eclipse Tribrid质谱与U3000 Nanolc系统代表下一个 LC-MS/MS系统的生成。该仪器提供更高的分辨率和更快的扫描速度，并且可以 执行并行获取以最大化占空比。我们的演示项目与热科学显示 实时搜索SPS MS3方法将识别率提高了30％，精度更高，更少 与Tulane现有的质谱系统的Q KERTIVE HF-X相比，MS2方法的干扰。这 通过数据依赖性采集（DDA）方法使用Eclipse的识别率增加了76％ Faims Pro界面处的蛋白质水平，并使用与数据无关的采集（DIA） - 增加了30％ - 基于无标签的方法。请求的电子转移分离方法可以更好地支持 - 翻译修改分析具有更自信的标识。新一代Tribrid MS给出了 实验性吞吐量在提供深度，覆盖范围和单细胞蛋白质组学功能的同时。这些 使用高级LC- MS/MS生物医学研究分析。 我们已经确定了由32位教职员工（12名主要用户和20个未成年人）的仪器用户群。 由NIH或其他联邦赠款。他们已要求拟议工具提供的功能进行执行 他们的研究表明了对所请求系统的重要需求。因此，满足这种需求是 预计将推进多个旨在促进跨越公共卫生的高影响力的生物医学研究项目 各种各样的主题领域和NIH研究的重点。