3D Encapsulation, Bioprinting and Controlled Delivery of Functionally Engineered EVs (FEEs)
功能工程电动汽车 (FEE) 的 3D 封装、生物打印和受控交付
基本信息
- 批准号:10433850
- 负责人:
- 金额:$ 46.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-18 至 2026-05-31
- 项目状态:未结题
- 来源:
- 关键词:3-Dimensional3D PrintActivities of Daily LivingAddressAlginatesAnti-Inflammatory AgentsArchitectureAreaBMP2 geneBindingBiological AssayBiological ModelsBiologyCalvariaCartilageCellsChronicComplexCuesDefectDentalDevelopmentDiseaseDoseEncapsulatedEngineeringEquilibriumEventExtracellular MatrixGenesGoalsGrowth FactorHealthHistologyHybridsHydrogelsImmunologyImpairmentIndividualInflammasomeInflammationInflammatoryInjuryInkKineticsKnowledgeLaboratoriesMADH7 geneMMP2 geneMeasurementMediator of activation proteinMesenchymal Stem CellsMicroRNAsModelingMuscleMusculoskeletalMusculoskeletal SystemNatural regenerationNervePathway interactionsPeptidesPlayPolymersProcessProductionPropertyRattusRegenerative MedicineRegenerative pathwayReporterRoleSignal TransductionSiteStructureSystemTechniquesTechnologyTestingTherapeuticTimeTissuesWorkbasebioprintingbody systemclinical translationcontrolled releasecraniofacialcraniofacial tissuecrosslinkcytokineextracellular vesiclesimmunoregulationimprovedin vivomacrophagemonomernovel strategiesparacrineprecision medicinepreventprotein expressionrecruitregenerativerepairedscaffoldspatiotemporalstem cell exosomesstem cell growthstem cell therapytissue injurytissue regenerationtissue repairtool
项目摘要
Summary:
Tissue repair is a complex process that involves a delicate temporal balance between inflammatory and
regenerative mechanisms. In health, initial inflammatory events are replaced by regenerative processes in a
coordinated manner. This sequence is disrupted in diseased states and complex injuries. The goal of
regenerative medicine is to reestablish this balance by preventing chronic/aberrant inflammation and promoting
repair and regeneration in a tissue-specific manner. While stem cell and growth factor therapies have been
explored for this purpose traditionally, recent studies highlight the immunomodulatory and protective functions
of mesenchymal stem cell derived extracellular vesicles (MSC EVs). Although MSC EVs possess versatile
properties, to engage tissue-specific pathways and fit the goals of precision medicine with translational
relevance, MSC EVs have to be engineered for enhanced pathway-specific functionality and delivered on site
in a spatially and temporally controlled manner. In this proposal, leveraging our preliminary results and our
expertise in EV biology, immunology and bone biology, we hypothesize that: Spatiotemporal control of
immunomodulatory and regenerative pathways can be achieved by selective incorporation of Functionally
Engineered EVs (FEEs)in 3D printed scaffolds. Using bone regeneration as a model system, we will test this
hypothesis in three specific aims. In aim 1, we will generate functionally engineered EVs (FEEs) that target
specific osteoinductive and immunomodulatory pathways. In aim 2, we will develop a photocrosslinkable
alginate-based delivery system with EV carrier and release motifs for spatial localization and temporally
controlled delivery of the FEEs developed in aim 1. In aim 3, we will utilize 3D printing technology to print
defined structures encapsulating the FEEs for spatially and temporally controlled biphasic delivery in vivo. This
system will be tested in a rat calvarial defect model. From the proposed studies, we will develop a platform
technology that can impact the field of regenerative medicine beyond the craniofacial and musculoskeletal
systems.
摘要:
组织修复是一个复杂的过程,它涉及炎症和炎症之间微妙的时间平衡。
再生机制。在健康中,最初的炎症事件被再生过程取代
协调一致的方式。这一序列在疾病状态和复杂的损伤中被打乱。的目标是
再生医学是通过预防慢性/异常炎症和促进
以特定于组织的方式修复和再生。而干细胞和生长因子疗法一直是
传统上对此进行了探索,最近的研究强调了免疫调节和保护功能
间充质干细胞来源的细胞外小泡(MSC EVS)。尽管MSC电动汽车拥有多功能
属性,以参与组织特异性途径,并与翻译的精确医学目标相匹配
相关性,MSC电动汽车必须设计为增强特定路径的功能并在现场交付
以空间和时间受控的方式。在这项提案中,利用我们的初步结果和我们的
在肠道病毒生物学、免疫学和骨骼生物学方面的专业知识,我们假设:时空控制
免疫调节和再生途径可以通过选择性地掺入功能
3D打印脚手架中的工程电动汽车(费用)。使用骨再生作为模型系统,我们将测试这一点
三个具体目标的假设。在目标1中,我们将生成功能工程化的电动汽车(费用),目标是
特定的骨诱导和免疫调节途径。在目标2中,我们将开发一种可光交联型
基于海藻酸盐的电动汽车载体和释放模体用于空间定位和时间定位的递送系统
目标1中开发的费用的受控交付。在目标3中,我们将利用3D打印技术来打印
限定的结构,封装了体内空间和时间受控两相给药的费用。这
该系统将在大鼠颅骨缺损模型中进行测试。根据建议的研究,我们将开发一个平台
可影响颅面和肌肉骨骼以外的再生医学领域的技术
系统。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LYNDON F COOPER其他文献
LYNDON F COOPER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LYNDON F COOPER', 18)}}的其他基金
3D Encapsulation, Bioprinting and Controlled Delivery of Functionally Engineered EVs (FEEs)
功能工程电动汽车 (FEE) 的 3D 封装、生物打印和受控交付
- 批准号:
10183784 - 财政年份:2021
- 资助金额:
$ 46.22万 - 项目类别:
3D Encapsulation, Bioprinting and Controlled Delivery of Functionally Engineered EVs (FEEs)
功能工程电动汽车 (FEE) 的 3D 封装、生物打印和受控交付
- 批准号:
10633258 - 财政年份:2021
- 资助金额:
$ 46.22万 - 项目类别:
Clinical/Translational Facilities for Innovative Oral Health Research at UIC
UIC创新口腔健康研究的临床/转化设施
- 批准号:
7899547 - 财政年份:2010
- 资助金额:
$ 46.22万 - 项目类别:
TRANSCRIPTIONAL CONTROL OF OSTEOBLAST STRESS RESPONSES
成骨细胞应激反应的转录控制
- 批准号:
2770251 - 财政年份:1996
- 资助金额:
$ 46.22万 - 项目类别:
TRANSCRIPTIONAL CONTROL OF OSTEOBLAST STRESS RESPONSES
成骨细胞应激反应的转录控制
- 批准号:
2128864 - 财政年份:1996
- 资助金额:
$ 46.22万 - 项目类别:
相似海外基金
Study on the use of 3D print models to improve understanding of geomorphic processes
研究使用 3D 打印模型来提高对地貌过程的理解
- 批准号:
22K13777 - 财政年份:2022
- 资助金额:
$ 46.22万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
3D print-on-demand technology for personalised medicines at the point of care
用于护理点个性化药物的 3D 按需打印技术
- 批准号:
10045111 - 财政年份:2022
- 资助金额:
$ 46.22万 - 项目类别:
Grant for R&D
Regenerative cooling optimisation in 3D-print rocket nozzles
3D 打印火箭喷嘴的再生冷却优化
- 批准号:
2749141 - 财政年份:2022
- 资助金额:
$ 46.22万 - 项目类别:
Studentship
Development of a New Powder Mix and Process Plan to 3D Print Ductile Iron Parts
开发用于 3D 打印球墨铸铁零件的新粉末混合物和工艺计划
- 批准号:
548945-2019 - 财政年份:2021
- 资助金额:
$ 46.22万 - 项目类别:
College - University Idea to Innovation Grants
Development of a New Powder Mix and Process Plan to 3D Print Ductile Iron Parts
开发用于 3D 打印球墨铸铁零件的新粉末混合物和工艺计划
- 批准号:
548945-2019 - 财政年份:2020
- 资助金额:
$ 46.22万 - 项目类别:
College - University Idea to Innovation Grants
Administrative Supplement for Equipment: 6-axis Positioner to Improve 3D Print Quality and Print Size
设备管理补充:用于提高 3D 打印质量和打印尺寸的 6 轴定位器
- 批准号:
10801667 - 财政年份:2019
- 资助金额:
$ 46.22万 - 项目类别:
SBIR Phase II: Pellet based 3D print extrusion process for shoe manufacturing
SBIR 第二阶段:用于制鞋的基于颗粒的 3D 打印挤出工艺
- 批准号:
1738138 - 财政年份:2017
- 资助金额:
$ 46.22万 - 项目类别:
Standard Grant
Development of "artificial muscle' ink for 3D print of microrobots
开发用于微型机器人3D打印的“人造肌肉”墨水
- 批准号:
17K18852 - 财政年份:2017
- 资助金额:
$ 46.22万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
I-Corps: Nanochon, a Commercial Venture to 3D Print Regenerative Implants for Joint Reconstruction
I-Corps:Nanochon,一家商业企业,致力于 3D 打印再生植入物进行关节重建
- 批准号:
1612567 - 财政年份:2016
- 资助金额:
$ 46.22万 - 项目类别:
Standard Grant
SBIR Phase I: Pellet based 3D print extrusion process for shoe manufacturing
SBIR 第一阶段:用于制鞋的基于颗粒的 3D 打印挤出工艺
- 批准号:
1621732 - 财政年份:2016
- 资助金额:
$ 46.22万 - 项目类别:
Standard Grant