Surgery / Physiology Core

外科/生理学核心

• 批准号: 10433817
• 负责人: Jiasheng Zhang
• 金额: $ 10.66万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-10 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10433817
• 关键词: Animal Model; Biological Assay; Biology; Blood Vessels; Carotid Arteries; Collaborations; Cornea; Diagnostic; Disease; Evaluation; Gene Targeting; Hindlimb; Human; Human Resources; Imaging Device; Ischemia; Lasers; Ligation; Measures; Methodology; Microsurgery; Modeling; Molecular; Mus; Operative Surgical Procedures; Pharmaceutical Preparations; Phenotype; Physiologic Monitoring; Physiology; Probability; Procedures; Protocols documentation; Research; Research Personnel; Services; Signal Pathway; Signal Transduction; Skin wound healing; Standardization; Stroke; Techniques; Therapeutic; Time; Training; Translations; Validation; animal care; design; experience; human disease; human model; matrigel; mechanotransduction; mouse model; programs; success; tool

Core C will continue to provide key animal models necessary for the studies of ERK signaling, miR 17-19 cluster biology, and matrix and mechanotransduction in arteriogenesis, and to facilitate evaluation and validation of discovery research carried out in the PPG projects. To this end, the Core has established 64 types of surgical animal models of human diseases. To meet the evolving needs of the program, we are introducing six mouse models including mouse hindlimb ischemia, stroke, Matrigel plug assay, skin wound healing, partial carotid ligation and cornea pocket assay. The Core is supported by 3 microsurgeons with extensive microsurgery techniques experience and 2 technologists with experiences in animal care and handling under the direction of Dr. Zhang. These standardized efforts will result in facilitating the translation of ideas from the bench to mice, and ultimately to humans.

Core C将继续提供ERK研究所需的关键动物模型 信号传导、miR 17-19 簇生物学以及动脉生成中的基质和机械转导，并促进 对 PPG 项目中进行的发现研究进行评估和验证。为此，核心 建立了64种人类疾病的外科动物模型。为了满足不断变化的需求 项目中，我们引入了六种小鼠模型，包括小鼠后肢缺血模型、中风模型、Matrigel栓塞模型 化验、皮肤伤口愈合、部分颈动脉结扎和角膜袋化验。核心由 3 名显微外科医生提供支持 拥有丰富的显微外科技术经验和2名具有动物经验的技术人员 在张医生的指导下进行护理和处理。这些标准化工作将促进 将想法从实验台转移到老鼠身上，并最终转移到人类身上。