Surgery / Physiology Core

外科/生理学核心

• 批准号: 10192385
• 负责人: Jiasheng Zhang
• 金额: $ 10.33万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-10 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10192385
• 关键词: Animal Model; Biological Assay; Biology; Blood Vessels; Carotid Arteries; Collaborations; Cornea; Diagnostic; Disease; Evaluation; Gene Targeting; Hindlimb; Human; Human Resources; Imaging Device; Ischemia; Lasers; Ligation; Measures; Methodology; Microsurgery; Modeling; Molecular; Mus; Operative Surgical Procedures; Pharmaceutical Preparations; Phenotype; Physiologic Monitoring; Physiology; Probability; Procedures; Protocols documentation; Research; Research Personnel; Services; Signal Pathway; Signal Transduction; Skin wound healing; Standardization; Stroke; Surgeon; Techniques; Therapeutic; Time; Training; Translations; Validation; animal care; design; experience; human disease; human model; matrigel; mechanotransduction; mouse model; programs; success; tool

Abstract/Summary: Core C will continue to provide key animal models necessary for the studies of ERK signaling, miR 17-19 cluster biology, and matrix and mechanotransduction in arteriogenesis, and to facilitate evaluation and validation of discovery research carried out in the PPG projects. To this end, the Core has established 64 types of surgical animal models of human diseases. To meet the evolving needs of the program, we are introducing six mouse models including mouse hindlimb ischemia, stroke, Matrigel plug assay, skin wound healing, partial carotid ligation and cornea pocket assay. The Core is supported by 3 micro- surgeons with extensive microsurgery techniques experience and 2 technologists with experiences in animal care and handling under the direction of Dr. Zhang. These standardized efforts will result in facilitating the translation of ideas from the bench to mice, and ultimately to humans.

摘要/综述：CORE C将继续为ERK研究提供必要的关键动物模型 信号，miR 17-19簇生物学，以及动脉形成中的基质和机械转导，并促进 对PPG项目中开展的发现研究进行评估和验证。为此，核心有 建立类人类疾病外科动物模型。以满足不断变化的需求 节目中，我们介绍了六种小鼠模型，包括小鼠后肢缺血、中风、Matrigel Plug 皮肤创面愈合、部分颈动脉结扎和角膜袋实验。核心由3个微处理器支持 具有丰富显微外科技术经验的外科医生和具有动物经验的2名技术专家 在张医生的指导下护理和处理。这些标准化的努力将导致促进 将想法从长凳上转化到老鼠身上，最终转化到人类身上。