Preclinical Development of ACXT-3102 for the Treatment of Pancreatic Adenocarcinoma (PDAC)

ACXT-3102 治疗胰腺癌 (PDAC) 的临床前开发

• 批准号: 10435565
• 负责人: WILLIAM G HAWKINS
• 金额: $ 101.92万
• 依托单位: ACCURONIX THERAPEUTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-23 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10435565
• 关键词: Anemia; Antineoplastic Agents; Biological Availability; Body Weight; Cancer Cell Growth; Cancer Etiology; Cancer Model; Cancer Patient; Canis familiaris; Cardiac; Cardiovascular system; Cell Death; Cells; Cessation of life; Chemicals; Clinical; Clinical Trials; Cystine; Data; Development; Disease; Dose; Dose-Limiting; Drug Delivery Systems; Drug Kinetics; Drug Stability; Drug Targeting; Enzymes; Fatigue; Formulation; Glutamates; Goals; Grant; Half-Life; Human; In Vitro; Incubated; Intravenous; Investigational Drugs; Life; Life Expectancy; Ligands; Malignant Neoplasms; Malignant neoplasm of pancreas; Measures; Mesylates; Metabolic; Metabolism; Microsomes; Modeling; Mus; No-Observed-Adverse-Effect Level; Oral; Oral Administration; Oxidative Stress; Paclitaxel; Pancreas; Pancreatic Adenocarcinoma; Patients; Pharmaceutical Preparations; Pharmacodynamics; Pharmacology; Pharmacology Study; Phase; Phase I Clinical Trials; Plasma; Play; Practice Guidelines; Quality of life; Rattus; Regimen; Research; Role; Safety; Schedule; Series; Site; Small Business Technology Transfer Research; Sodium Chloride; Solid; Solubility; Survival Rate; Temperature; Testing; Therapeutic; Therapeutic Index; Time; Toxic effect; Toxicology; Treatment Efficacy; Tumor Volume; Universities; Ventricular; Washington; Work; antiporter; aqueous; base; cancer cell; cancer type; chemical stability; commercial application; cytotoxicity; design; drug synthesis; effective therapy; erastin; first-in-human; gemcitabine; good laboratory practice; improved; in vitro Assay; in vivo; malic enzyme; medical schools; molecular marker; mouse model; nerve damage; novel; overexpression; oxaliplatin; pancreatic cancer cells; pancreatic cancer model; pancreatic cancer patients; pharmacokinetics and pharmacodynamics; physical property; pre-clinical; preclinical development; preclinical study; prevent; programs; side effect; sigma-2 receptor; small molecule; standard of care; tumor; tumor growth; tumor metabolism; uptake

Project Summary/Abstract Pancreatic cancer is a devastating disease with a very low (8%) 5-year survival rate. Therapeutic options are limited in efficacy and many have substantial toxicity. Targeted drug delivery may improve the therapeutic index of cancer drugs by enhancing drug localization to the cancer cell while minimizing off-target side effects. Sigma-2 receptors (S2R) are highly expressed in pancreatic and other cancers compared to healthy cells. Accuronix Therapeutics is developing ACXT-3102, a molecule with therapeutic potential licensed from Washington University School of Medicine in St. Louis (WUSM). ACXT-3102 is comprised of a S2 ligand covalently bound to the ferroptosis-inducing molecule dm-erastin. Preliminary data show that ACXT-3102 increased the cytotoxicity against pancreatic tumor cells in vitro by 35-fold compared to dm-erastin alone. ACXT-3102 has tremendous potential as a novel treatment option for pancreatic and perhaps several other types of cancer. Drug optimization strategies were conducted in a Phase I STTR which resulted in a better yield during drug synthesis, identification of a mesylate salt with high aqueous solubility and improved physical properties suitable for oral drug administration, demonstration of good oral efficacy and discovery that tumors lacking malic enzyme 1 (ME1) are even more sensitive to ACXT-3102. For this Phase II STTR program, Accuronix Therapeutics will continue to work with our research colleagues from WUSM to prepare ACXT-3102 for Investigational New Drug (IND) submission and eventually testing the compound in pancreatic cancer patients. We will conduct a series of preclinical studies to optimize the dosing regimen in murine pancreatic cancer models – understanding if once, twice or three times per day drug administration improves plasma exposure and anti-tumor efficacy while maintaining safety, i.e., avoiding dose- limiting side effects. Studies will also explore if efficacy of our drug can be further improved when given in combination with gemcitabine, a current standard-of-care for pancreatic cancer. Chemical and metabolic stability of ACXT-3102 will be established using in vitro assays to guide storage conditions of the drug and understand which preclinical species best represents metabolism in humans. To obtain data required for the pharmacology and safety sections of the IND package, preclinical studies will be conducted according to Good Laboratory Practice (GLP) guidelines to generate pharmacokinetic (PK) and pharmacodynamic (PD) data for correlating plasma exposures in different species to what is predicted in human. Similarly, formal GLP toxicology studies will be completed using three different doses in rats and dogs to establish the “No Observed Adverse Effect Level” (NOAEL). These data will be used to model exposure levels in humans and establish a safe, first-in-human (FIH) dose for starting our clinical trials in cancer patients. At the conclusion of the Phase II STTR grant, we will have the pharmacology and safety information on ACXT-3102 required for the IND package, and will have established the first dose to be used in patients.

项目总结/文摘