HIV modulation of BAG3 impacting quality control of Tau in neuronal cells

HIV 对 BAG3 的调节影响神经元细胞中 Tau 的质量控制

• 批准号: 10437950
• 负责人: Kamel Khalili
• 金额: $ 39.63万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10437950
• 关键词: Affect; Alzheimer' s Disease; Alzheimer' s disease brain; Animal Model; Animals; Apoptosis; Attention; Autophagocytosis; BAG3 gene; Biochemical; Bioenergetics; Brain; CRISPR/Cas technology; Calcium Signaling; Cause of Death; Cell Culture Techniques; Cell physiology; Cells; Clinical Research; Clustered Regularly Interspaced Short Palindromic Repeats; Data; Deposition; Development; Disease; Down-Regulation; Energy Metabolism; Ensure; Environment; Equilibrium; Event; Excision; Generations; Genome; HIV; HIV Infections; HIV Seronegativity; HIV Seropositivity; HIV tat Protein; HIV-1; HIV-associated neurocognitive disorder; Heat-Shock Proteins 70; Homeostasis; In Vitro; Infection; Interruption; Investigation; Laboratories; Life; Light; Mediating; Membrane Potentials; Memory; Microelectrodes; Microtubule-Associated Proteins; Microtubules; Mitochondria; Modeling; Molecular; Molecular Chaperones; Neurodegenerative Disorders; Neurofibrillary Tangles; Neurons; Organelles; Outcome; Outcome Study; Parkin; Pathogenesis; Pathogenicity; Pathway interactions; Patients; Physiological; Process; Production; Proteins; Quality Control; Risk; Risk Factors; Role; Signal Transduction; Stress; Stress-Induced Protein; System; Thinking; Toxic effect; Transgenic Animals; United States; Up-Regulation; Viral Proteins; Virus; aging brain; antiretroviral therapy; brain tissue; endoplasmic reticulum stress; experimental study; human old age (65+); in vivo; interest; misfolded protein; mitochondrial membrane; mutant; neuron loss; neuronal circuitry; neurotoxic; premature; protein aggregation; proteostasis; small hairpin RNA; tat Protein; tau Proteins; tau aggregation; tau phosphorylation; tau-1; transmission process; treatment effect

PROJECT SUMMARY It is estimated that more than 10% of the 1.1 million HIV positive patients in United States will be over 65 years old in less than ten years, a critical stage in life where the the risk for developing Alzheimer’s disease (AD) increases. Earlier results from clinical studies showed several abnormalities including difficulties with memory, thinking, and reasoning become more common in older HIV positive patients, implying that their risk factors may be elevated when compared with their HIV negative counterparts. This notion brings up the concept that HIV infection, even in patients whose virus is well controlled by antiretroviral therapy (ART), may be more prone to developing neurodegenerative disorders including AD. Considering the inability of ART to effectively suppress expression of viral proteins, including HIV Tat, which is a profoundly neurotoxic protein, we envision a scenario in which, by dysregulating the protein quality control (PQC) pathway, Tat may perturb homeostasis of key proteins associated with the pathogenesis of AD and set the stage for the development of disease. Several data from our and other laboratories support this concept. First, we demonstrated that Tat inhibits expression of BAG3, a co-chaperone/partner of HSP70 that is involved in the removal of dysfunctional and obsolete organelles including mitochondria through a process called mitophagy, and participates in autophagy and clearance of damaged and misfolded proteins by the protein quality control (PQC) pathway. Second, activation of BAG3 is concurrent with a decrease in the level of phosph-tau and an increase in the clearance of tau in neuronal cells. Third, soon after destabilization of microtubules, tau associates with Hsp70/Hsc70, a key partner of BAG3. Fourth, induction of Tat in the brains of transgenic animals increases accumulation of phospho-tau. Thus, Tat- mediated reduction in the level of BAG3 may have a damaging effect on neuronal cells by interrupting the process that ensures intracellular removal of the toxic from of tau, yet maintains the healthy species of tau that is critical for neuronal cell function. Hyper-phosphorylation of tau, which contributes to its misfolding and toxicity, may be attributed to Tat via induction of ROS, ER stress, and mitochondrial dysfunctionality. The latter is of particular interest as truncated tau has been implicated in dysregulation of mitochondrial dynamics and healty energy metabolism. These observations prompted us to hypothesize that, on one hand, Tat impacts the quality and concentration of proper levels of functional tau and the clearance of its toxic form by suppressing expression of BAG3, and on the other hand, Tat contributes to the generation of the toxic tau and dysfunctionality of bioenergetic pathways and mitochondria by inducing stress conditions in cells. To examine this model, we will employ in vitro primary neuronal cultures, ex vivo animal brain tissue, and an in vivo animal model to unravel the underlying molecular basis of HIV-1/Tat-induced perturbation of PQC and homeostasis of tau in neuronal cell function.

项目总结