Botanical Core

项目概要/摘要 研究核心B的目的是支持ODS/NCCIH U19植物性膳食的总体工作。 补充研究中心（BDSRC）在两个具体方面：（1）通过集中采购， 使用的多酚材料的表征、存档、质量控制和有效期/稳定性评估 单个研究项目;以及（2）通过对所有中心研究项目的集中生物分析支持， 丹我们以前的研究已经证明了从一个饮食补充剂的显着效果， 葡萄籽多酚提取物（GSPE）、康科德葡萄汁（CGJ）和白藜芦醇（RSV）的组合， 促进在选定的压力条件下保持认知健康和心理弹性。我们 还表明，从这些植物中提取的特定代谢物可以在血液和大脑中检测到 组织.我们可以检测各种类黄酮的甲基化和/或葡萄糖醛酸化衍生物 （黄烷-3-醇如儿茶素和表儿茶素;花青素（矢车菊色素和锦葵色素）;黄酮醇（槲皮素）; 芪类（白藜芦醇））。核心B将负责母体的鉴别和定量 化合物和衍生代谢物的动物和人类生物体液和组织，使用各种分析 技术，包括与不同质谱（MS）技术偶联的液相色谱（LC），例如 高分辨质谱（HRMS）和三重四极杆串联质谱（QQQ-MS/MS）， 1D核磁共振（NMR）技术，包括1H NMR和13 C NMR，以及各种2D NMR 技术包括1H-1H TOCSY（总相关光谱）、1H-1H NOESY（核Overhauser 增强光谱法）、梯度增强1H-1H COSY（相关光谱法）、1H-13 C HSQC （杂原子单量子相干性）和1H-13 C HMBC（杂原子多重键相干性），以及 根据需要进行LC-NMR。除了这些生物利用度和PK研究外，核心B也将作为存档研究。 整个中心使用的所有植物材料、标准品和多酚化合物的储存库， 使用LIMS系统跟踪所有样品，并进行质量控制分析和稳定性测试 以支持这一职能。内部各调查员专门知识的互补性 核心B创建一个强大的协同团队，能够支持个人研究项目和协作 努力实现U19 BDSRC目标。

Project Summary/Abstract The purpose of Research Core B is to support the overall effort of the ODS/NCCIH U19 Botanical Dietary Supplement Research Center (BDSRC) in two specific areas: (1) by centralizing the procurement, characterization, archiving, quality control and shelf-life/stability assessment of polyphenolic materials used by individual Research Projects; and (2) by centralizing bioanalytical support for all Center Research Projects and Cores. Our previous studies have demonstrated the remarkable efficacy of dietary supplementation from a combination of grape seed polyphenolic extract (GSPE), Concord grape juice (CGJ), and resveratrol (RSV) in promoting preservation of cognitive wellness and psychological resiliency under select stress conditions. We have also shown that specific metabolites extracted from these botanicals can be detected in blood and brain tissue. We can detect methylated and/or glucuronidated derivatives of the various classes of flavonoids (flavan-3-ols such as catechin and epicatechin; anthocyanins (cyanidin and malvidin); flavonols (quercetin); stilbenes (resveratrol)). Core B will be responsible for the identification and quantitation of the parent compounds and derived metabolites in animal and human biological fluids and tissues, using various analytical techniques including liquid chromatography (LC) coupled to different mass spectrometric (MS) techniques, e.g. high resolution mass spectrometry (HRMS) and triple quadrupole tandem mass spectrometry (QqQ-MS/MS), 1D nuclear magenetic resonance (NMR) techniques including 1H NMR and 13C NMR, and various 2D NMR techniques including 1H-1H TOCSY (total correlated spectroscopy), 1H-1H NOESY (nuclear Overhauser enhancement spectroscopy), gradient enhanced 1H-1H COSY (correlated spectroscopy), 1H-13C HSQC (heteronuclear single quantum coherence), and 1H-13C HMBC (heteronuclear multiple bond coherence), and LC-NMR as needed. In addition to these bioavailability and PK studies, Core B will also serve as an archived repository of all botanical materials, standards and polyphenolic compounds used throughout the Center, maintain tracking of all samples using a LIMS system, and perform quality control analyses and stability testing in support of this function. The complementary nature of the expertise of the individual investigators within Core B creates a strong synergistic team capable of supporting individual Research Projects and collaborative efforts focused on achieving U19 BDSRC objectives.