Botanical Core

植物核心

• 批准号: 10447075
• 负责人: James E Simon
• 金额: $ 18.98万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10447075
• 关键词: Behavioral; Biological; Botanical dietary supplements; Botanicals; resilience

Project Summary/Abstract The purpose of Research Core B is to support the overall effort of the ODS/NCCIH U19 Botanical Dietary Supplement Research Center (BDSRC) in two specific areas: (1) by centralizing the procurement, characterization, archiving, quality control and shelf-life/stability assessment of polyphenolic materials used by individual Research Projects; and (2) by centralizing bioanalytical support for all Center Research Projects and Cores. Our previous studies have demonstrated the remarkable efficacy of dietary supplementation from a combination of grape seed polyphenolic extract (GSPE), Concord grape juice (CGJ), and resveratrol (RSV) in promoting preservation of cognitive wellness and psychological resiliency under select stress conditions. We have also shown that specific metabolites extracted from these botanicals can be detected in blood and brain tissue. We can detect methylated and/or glucuronidated derivatives of the various classes of flavonoids (flavan-3-ols such as catechin and epicatechin; anthocyanins (cyanidin and malvidin); flavonols (quercetin); stilbenes (resveratrol)). Core B will be responsible for the identification and quantitation of the parent compounds and derived metabolites in animal and human biological fluids and tissues, using various analytical techniques including liquid chromatography (LC) coupled to different mass spectrometric (MS) techniques, e.g. high resolution mass spectrometry (HRMS) and triple quadrupole tandem mass spectrometry (QqQ-MS/MS), 1D nuclear magenetic resonance (NMR) techniques including 1H NMR and 13C NMR, and various 2D NMR techniques including 1H-1H TOCSY (total correlated spectroscopy), 1H-1H NOESY (nuclear Overhauser enhancement spectroscopy), gradient enhanced 1H-1H COSY (correlated spectroscopy), 1H-13C HSQC (heteronuclear single quantum coherence), and 1H-13C HMBC (heteronuclear multiple bond coherence), and LC-NMR as needed. In addition to these bioavailability and PK studies, Core B will also serve as an archived repository of all botanical materials, standards and polyphenolic compounds used throughout the Center, maintain tracking of all samples using a LIMS system, and perform quality control analyses and stability testing in support of this function. The complementary nature of the expertise of the individual investigators within Core B creates a strong synergistic team capable of supporting individual Research Projects and collaborative efforts focused on achieving U19 BDSRC objectives.

项目概要/摘要 研究核心 B 的目的是支持 ODS/NCCIH U19 植物膳食的整体努力 补充研究中心（BDSRC）在两个具体领域：（1）通过集中采购， 所用多酚材料的表征、存档、质量控制和保质期/稳定性评估 个人研究项目； (2) 集中对所有中心研究项目的生物分析支持，以及 核心。我们之前的研究已经证明了膳食补充剂的显着功效 葡萄籽多酚提取物 (GSPE)、Concord 葡萄汁 (CGJ) 和白藜芦醇 (RSV) 的组合 促进在特定压力条件下保持认知健康和心理弹性。我们 还表明，可以在血液和大脑中检测到从这些植物中提取的特定代谢物 组织。我们可以检测各类黄酮类化合物的甲基化和/或葡萄糖醛酸化衍生物 (黄烷-3-醇，例如儿茶素和表儿茶素；花青素(花青素和锦葵素)；黄酮醇(槲皮素)； 二苯乙烯（白藜芦醇））。核心B将负责母体的鉴定和定量 使用各种分析方法，对动物和人类生物体液和组织中的化合物和衍生代谢物进行分析 技术，包括液相色谱 (LC) 与不同的质谱 (MS) 技术相结合，例如 高分辨率质谱（HRMS）和三重四极杆串联质谱（QqQ-MS/MS）， 一维核磁共振 (NMR) 技术，包括 1H NMR 和 13C NMR，以及各种 2D NMR 技术包括 1H-1H TOCSY（全相关光谱）、1H-1H NOESY（核 Overhauser 增强光谱）、梯度增强 1H-1H COSY（相关光谱）、1H-13C HSQC （异核单量子相干）和 1H-13C HMBC（异核多键相干），以及 根据需要进行 LC-NMR。除了这些生物利用度和 PK 研究之外，Core B 还将作为存档 整个中心使用的所有植物材料、标准品和多酚化合物的存储库， 使用 LIMS 系统跟踪所有样品，并进行质量控制分析和稳定性测试 以支持该功能。各个研究人员的专业知识具有互补性 核心 B 创建了一个强大的协同团队，能够支持个人研究项目和协作 努力的重点是实现 U19 BDSRC 目标。