Core C: Microbial Engineering and Transplantation Core

微生物工程与移植核心(核心C)。摘要： 微生物工程和移植核心的总体目标是维护和发展 细菌菌株，包括模拟合成群落，并提供复杂的多菌种 移植社区，以及协助灵知生物体重建的所有方面 (微生物移植)针对项目调查人员的研究。此PPG中的所有项目都涉及 移植天然或基因操纵的细菌种类，或作为多菌剂单独移植 群落和/或具有选择性代谢物产生能力的合成细菌群落(或 功能缺失突变体)转化为无菌小鼠(即诺生菌重组)。厌氧培养和 微生物工程，以及无菌小鼠的衍生、维护和微生物移植，是 复杂实验的所有复杂功能都需要专业知识和基础设施。这个 微生物工程和移植核心公司将为这一PPG的研究人员提供所有这些服务。 核心的微生物培养和工程部分将帮助调查人员集中 体外代谢/生化和体内微生物移植的厌氧培养能力 学习。每个项目还将利用这一核心，以获得其在基因工程方面的专门知识和援助 根据需要进行人类共生。这一核心的灵知生物部分将有助于所有项目的执行。 这些研究包括诺维菌素重组研究及其与心脏代谢表型研究的接口(在核心D中)。

Microbial Engineering and Transplantation Core (Core C). ABSTRACT: The overall goals of the Microbial Engineering and Transplantation Core are to maintain and develop bacterial strains, including model synthetic communities, and provide complex polymicrobial communities for transplantation, as well as to assist in all aspects of gnotobiotic reconstitution (microbial transplantation) studies for Project Investigators. All projects in this PPG involve transplantation of native or genetically manipulated bacterial species, either singly, as polymicrobial communities, and/or synthetic bacterial communities harboring selective metabolite-producing capabilities (or functional deletion mutants) into germ-free mice (i.e. gnotobiotic reconstitution). Anaerobic culturing and microbial engineering, as well as germ-free mouse derivation, maintenance, and microbial transplantation, are all complex features of intricate experiments that require specialized expertise, and infrastructure. The Microbial Engineering and Transplantation Core will provide all of these services to investigators of this PPG. The microbial culturing and engineering component of the Core will assist investigators with centralized anaerobic culturing capabilities for both in vitro metabolic/biochemical, and in vivo microbial transplantation studies. Each Project will also draw on this core for its expertise and assistance with genetic engineering of human commensals as needed. The gnotobiotics component of this core will assist all Projects in performance of gnotobiotic reconstitution studies, and their interface with cardiometabolic phenotyping studies (in Core D).