Core C: Microbial Engineering and Transplantation Core

微生物工程和移植核心（核心C）。摘要： 微生物工程和移植核心的总体目标是保持和发展 细菌菌株，包括模型合成社区，并提供复杂的多微生物 社区的移植，以及协助各方面的gnotobiotic重建 （微生物移植）研究。本PPG中的所有项目均涉及 移植天然或遗传操作的细菌物种，无论是单独的，作为多微生物 群落和/或具有选择性代谢物产生能力的合成细菌群落（或 功能缺失突变体）转化到无菌小鼠中（即无菌重建）。厌氧培养和 微生物工程，以及无菌小鼠的衍生、维持和微生物移植， 复杂实验的所有复杂特征都需要专门的专业知识和基础设施。的 微生物工程和移植中心将为本PPG的研究者提供所有这些服务。 核心的微生物培养和工程组件将协助研究人员集中 用于体外代谢/生物化学和体内微生物移植的厌氧培养能力 问题研究每个项目还将利用这一核心的专门知识和援助， 需要的人的身体该核心的gnotobiotics成分将有助于所有项目的性能 gnotobiotic重建研究，及其与心脏代谢表型研究的接口（核心D）。

Microbial Engineering and Transplantation Core (Core C). ABSTRACT: The overall goals of the Microbial Engineering and Transplantation Core are to maintain and develop bacterial strains, including model synthetic communities, and provide complex polymicrobial communities for transplantation, as well as to assist in all aspects of gnotobiotic reconstitution (microbial transplantation) studies for Project Investigators. All projects in this PPG involve transplantation of native or genetically manipulated bacterial species, either singly, as polymicrobial communities, and/or synthetic bacterial communities harboring selective metabolite-producing capabilities (or functional deletion mutants) into germ-free mice (i.e. gnotobiotic reconstitution). Anaerobic culturing and microbial engineering, as well as germ-free mouse derivation, maintenance, and microbial transplantation, are all complex features of intricate experiments that require specialized expertise, and infrastructure. The Microbial Engineering and Transplantation Core will provide all of these services to investigators of this PPG. The microbial culturing and engineering component of the Core will assist investigators with centralized anaerobic culturing capabilities for both in vitro metabolic/biochemical, and in vivo microbial transplantation studies. Each Project will also draw on this core for its expertise and assistance with genetic engineering of human commensals as needed. The gnotobiotics component of this core will assist all Projects in performance of gnotobiotic reconstitution studies, and their interface with cardiometabolic phenotyping studies (in Core D).