Mouse Core

这个核心的总体目标是产生、繁殖、分型和提供转基因小鼠给 西北部地区的调查人员。由该核心开发和管理的独特资源以及增加的 对这些资源的需求和预期利用促使创造了一只单独的转基因小鼠 为此次竞争更新提供核心支持。这一核心的具体目标是： 目的1)建议RCE的研究人员在他们的研究中适当使用小鼠模型。 目的2)现有突变小鼠品系和新一代小鼠的育种、饲养和基因分型 通过对现有突变小鼠品系进行交叉杂交和基因分型来合成遗传突变。 目的3)建立新的转基因小鼠。 目的4)利用A‘SPEED从混合遗传背景的小鼠中快速产生同源小鼠 类基因的方法。

The overall objective of this core is to generate, breed, genotype and provide genetically modified mice to investigators of the NWRCE. The unique resources developed and managed by this core and the increased needs and anticipated utilization of these resources motivated the creation of a separate transgenic mouse core for this competitive renewal. The Specific Aims of this core are: Aim 1) Advise investigators of the RCE on appropriate use of mouse models for their studies. Aim 2) Breeding, husbandry, and genotyping of existing mutant mouse lines and generation of new compound genetic mutants through intercrossing and genotyping of existing mutant mouse lines. Aim 3) Generation of new genetically modified mice. Aim 4) Rapid generation of congenic mice from mice on mixed genetic backgrounds using a 'speed congenics' approach.