Mouse Core

鼠标核心

• 批准号: 8236983
• 负责人: ADELINE M HAJJAR
• 金额: $ 33.21万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8236983
• 关键词: Adjuvant; Affect; Alkaline Phosphatase; Breeding; Budgets; Burkholderia; Centers of Research Excellence; Communicable Diseases; Congenic Mice; Emerging Communicable Diseases; Funding; Generations; Genes; Genetic; Genotype; Human; Idaho; Immune; Immunity; Infection; Intervention; Intestines; Lipid A; Modeling; Mus; Mutant Strains Mice; Natural Immunity; Oregon; Pathogenesis; Plague; Pneumonia; Research Personnel; Resources; Role; Speed; TLR4 gene; Therapeutic Effect; Transgenic Mice; Universities; Variant; Washington; acquired immunity; biodefense; congenic; mouse model; mutant; pathogen; prophylactic; response

The overall objective of this core is to generate, breed, genotype and provide genetically modified mice to investigators of the NWRCE. The unique resources developed and managed by this core and the increased needs and anticipated utilization of these resources motivated the creation of a separate transgenic mouse core for this competitive renewal. The Specific Aims of this core are: Aim 1) Advise investigators of the RCE on appropriate use of mouse models for their studies. Aim 2) Breeding, husbandry, and genotyping of existing mutant mouse lines and generation of new compound genetic mutants through intercrossing and genotyping of existing mutant mouse lines. Aim 3) Generation of new genetically modified mice. Aim 4) Rapid generation of congenic mice from mice on mixed genetic backgrounds using a 'speed congenics' approach.

这个核心的总体目标是产生，繁殖，基因型和提供转基因小鼠， NWRCE的调查员。由该核心开发和管理的独特资源以及 对这些资源的需求和预期利用促使了单独的转基因小鼠的产生 这一竞争力的复苏。该核心的具体目标是： 目的1）建议RCE的研究人员在其研究中适当使用小鼠模型。 目的2）现有突变小鼠品系的育种、饲养和基因分型以及新突变小鼠品系的产生。 复合遗传突变体通过交叉和基因分型现有的突变小鼠品系。 目的3）获得新的转基因小鼠。 目的4）利用"speed“方法从混合遗传背景小鼠中快速繁殖同源小鼠 遗传学方法。