Neuroimaging & Neurocircuitry Core

神经影像学

• 批准号: 10450697
• 负责人: Timothy P Roberts
• 金额: $ 18.16万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10450697
• 关键词: Achievement; Address; Affect; Animal Model; Basal Ganglia; Behavior; Behavior assessment; Behavioral; Biological Markers; Biometry; Blinded; Brain; Brain Pathology; Brain imaging; Calcium; Cells; Central Nervous System Diseases; Cerebral Palsy; Clinical Trials; Collection; Consult; Consultations; Country; Coupled; DNA Sequence Alteration; Data; Data Science; Data Set; Databases; Development; Developmental Disabilities; Diagnostic; Disease; Dyes; Eligibility Determination; Epilepsy; Experimental Designs; Functional Imaging; Functional Magnetic Resonance Imaging; Functional disorder; Generations; Genes; Genetic; Genetic Variation; Goals; Growth; Human; Image; In Vitro; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Investigation; Limbic System; Magnetic Resonance Imaging; Magnetoencephalography; Mediating; Meta-Analysis; Methodology; Methods; Mission; Modality; Modeling; Monitor; Mus; Mutation; Neocortex; Neuraxis; Neurons; Neurosciences; Patients; Persons; Physiology; Population; Pre-Clinical Model; Procedures; Protocols documentation; Quality Control; Research; Research Design; Research Personnel; Research Support; Series; Services; Slice; Statistical Data Interpretation; Stratification; Structure; Symptoms; Techniques; Technology; Tissues; Training; autism spectrum disorder; base; brain behavior; brain circuitry; cognitive function; data sharing; experimental analysis; human model; imaging study; improved; in vivo; indexing; induced pluripotent stem cell; member; microscopic imaging; multimodality; multiphoton microscopy; neural circuit; neurochemistry; neuroimaging; neuronal circuitry; neurophysiology; novel; novel strategies; operation; patch clamp; power analysis; pre-clinical; prognostic; programs; protein expression; relating to nervous system; response; spectroscopic imaging; stem cells; temporal measurement; tissue culture; translational approach; translational therapeutics; voltage

(CORE E- NNC: NEUROIMAGING AND NEUROCIRCUITRY CORE) PROJECT SUMMARY Description: The Neuroimaging and Neurocircuitry Core (NNC) enables the study of changes in brain circuitry which occur in people with IDD. The Core supports research in both patients and animal models by providing users the following state-of-the-art technologies: (a) Magnetoencephalographic (MEG) recordings of brain activity; (b) Magnetic resonance imaging (MRI) and spectroscopy (MRS) studies of brain structure and function; (c) Multiphoton, confocal, and epifluorescence microscopic imaging studies of the dynamics of neuronal circuit function; (d) Patch clamp recording studies in neurons; and (e) Electroencephalographic (EEG) and other in vivo recordings. Analyses are performed in affected human patients with IDD, in Preclinical animal models of IDD, and in vitro (stem cells and tissue culture). Core Directors provide users with access to a wide set of otherwise inaccessible neuronal circuit assessment techniques, as well as expert consultation regarding experimental design and analysis. In all core functions, emphasis is placed upon quality control. In addition to assuring that all studies are competently conducted and appropriately analyzed, the Core Directors assist users to design studies that are properly controlled, adequately powered, and, when feasible, conducted in a blinded fashion. Relevance to IDDRC Mission: The overarching theme of this IDDRC is “Genes, Brain & Behavior” (see Overview). This Core focuses on each of these overlapping domains. Genetic alterations underlie many IDDs, and result in aberrant protein expression, which alters basic brain synaptic and circuit function. This in turn generates the behavioral anomalies which constitute IDD. The study of patients and animal models brings the genetic components into sharp focus, and the NNC facilitates examination into how these genetic changes alter brain function and, subsequently, whether such changes are correlated to behavior. Thus, the goal of the Core is to quantify the changes in function of neuronal circuits which result from genetic mutation and variation. Elucidating the changes in dynamic function of neuronal circuitry responsible for the generation of the atypical behaviors collectively termed the IDD is critical in translational efforts to provide diagnostic and prognostic biological markers (biomarkers) as well as biologically-based indices to serve as stratification biomarkers for clinical trial enrichment and response monitoring with the ultimate goal of developing better therapies to treat and cure brain pathologies underlying the IDDs and other related CNS disorders such as autism, epilepsy, and cerebral palsy. Eligibility: These services are available both to approved users of the IDDRC at CHOP/UPenn and to users at other Centers in the nationwide Network.

（核心E-NNC：神经影像学和神经记录核心） 项目摘要 描述：神经影像学和神经记录核心（NNC）可以研究脑电路的变化 这发生在患有IDD的人中。核心通过提供 用户以下最先进的技术：（a）磁脑（MEG）大脑的录音 活动; （b）磁共振成像（MRI）和光谱学（MRS）研究脑结构和功能； （C）神经元电路动力学的多光子，共聚焦和落荧光显微影像学研究 功能; （d）神经元中的斑块夹记录研究； （e）脑电图（EEG）和其他体内 录音。在IDD的临床前动物模型中，对患有IDD患者的患者进行分析， 和体外（干细胞和组织培养）。核心主管为用户提供了广泛的访问权限 无法访问的神经元电路评估技术以及有关实验的专家咨询 设计和分析。在所有核心功能中，重点都放在质量控制上。除了确保所有人 对研究进行了全面的进行和适当的分析，核心主管协助用户设计研究 可以正确控制，充分的动力，并且在可行的情况下以盲目的方式进行。 与IDDRC任务相关：此IDDRC的总体主题是“基因，大脑和行为”（请参阅 概述）。该核心专注于这些重叠域中的每个核心。遗传改变是许多IDD的基础， 并导致异常的蛋白质表达，从而改变了基本的大脑突触和电路功能。反过来 生成构成IDD的行为异常。对患者和动物模型的研究带来了 遗传成分分解为敏锐的焦点，NNC促进了这些遗传变化如何改变的检查 大脑功能，随后，这种变化是否与行为相关。那，核心的目标 是为了量化由基因突变和变异导致的神经元回路功能的变化。 阐明负责非典型神经元电路动态功能的变化 集体称为IDD的行为对于提供诊断和预后的翻译工作至关重要 生物标记物（生物标志物）以及基于生物学的指数，可作为分层生物标志物 临床试验丰富和反应监测的最终目标是开发更好的治疗疗法 并治愈IDD和其他相关CNS疾病（例如自闭症，癫痫和 脑瘫。 资格：这些服务既可以用于Chop/Upenn的IDDRC的批准用户，又可以使用 全国网络中的其他中心。