Neuroimaging & Neurocircuitry Core

神经影像学

• 批准号: 10678901
• 负责人: Timothy P Roberts
• 金额: $ 18.16万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10678901
• 关键词: Achievement; Address; Affect; Ally; Animal Model; Basal Ganglia; Behavior; Behavior assessment; Behavioral; Biological Markers; Biometry; Blinded; Brain; Brain Pathology; Brain imaging; Calcium; Cells; Central Nervous System; Central Nervous System Diseases; Cerebral Palsy; Clinical Trials; Collection; Consult; Consultations; Country; Coupled; DNA Sequence Alteration; Data; Data Science; Data Set; Databases; Development; Developmental Disabilities; Diagnostic; Disease; Dyes; Electroencephalography; Eligibility Determination; Epilepsy; Experimental Designs; Functional Imaging; Functional Magnetic Resonance Imaging; Functional disorder; Generations; Genes; Genetic; Genetic Variation; Goals; Growth; Human; Image; In Vitro; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Investigation; Limbic System; Magnetic Resonance Imaging; Magnetoencephalography; Mediating; Meta-Analysis; Methodology; Methods; Mission; Modality; Modeling; Monitor; Mus; Mutation; Neocortex; Neurons; Neurosciences; Patients; Persons; Physiology; Population; Pre-Clinical Model; Procedures; Protocols documentation; Quality Control; Research; Research Design; Research Personnel; Research Support; Series; Services; Slice; Spectrum Analysis; Statistical Data Interpretation; Stratification; Structure; Symptoms; Synapses; Techniques; Technology; Tissues; Training; autism spectrum disorder; brain circuitry; cognitive function; data sharing; experimental analysis; human model; imaging study; improved; in vivo; indexing; induced pluripotent stem cell; member; microscopic imaging; multi-photon; multimodality; multiphoton microscopy; neocortical; neural; neural circuit; neurochemistry; neuroimaging; neuronal circuitry; neurophysiology; novel; novel strategies; operation; patch clamp; power analysis; pre-clinical; prognostic; programs; protein expression; response; stem cells; structural imaging; temporal measurement; tissue culture; translational approach; translational therapeutics; voltage

(CORE E- NNC: NEUROIMAGING AND NEUROCIRCUITRY CORE) PROJECT SUMMARY Description: The Neuroimaging and Neurocircuitry Core (NNC) enables the study of changes in brain circuitry which occur in people with IDD. The Core supports research in both patients and animal models by providing users the following state-of-the-art technologies: (a) Magnetoencephalographic (MEG) recordings of brain activity; (b) Magnetic resonance imaging (MRI) and spectroscopy (MRS) studies of brain structure and function; (c) Multiphoton, confocal, and epifluorescence microscopic imaging studies of the dynamics of neuronal circuit function; (d) Patch clamp recording studies in neurons; and (e) Electroencephalographic (EEG) and other in vivo recordings. Analyses are performed in affected human patients with IDD, in Preclinical animal models of IDD, and in vitro (stem cells and tissue culture). Core Directors provide users with access to a wide set of otherwise inaccessible neuronal circuit assessment techniques, as well as expert consultation regarding experimental design and analysis. In all core functions, emphasis is placed upon quality control. In addition to assuring that all studies are competently conducted and appropriately analyzed, the Core Directors assist users to design studies that are properly controlled, adequately powered, and, when feasible, conducted in a blinded fashion. Relevance to IDDRC Mission: The overarching theme of this IDDRC is “Genes, Brain & Behavior” (see Overview). This Core focuses on each of these overlapping domains. Genetic alterations underlie many IDDs, and result in aberrant protein expression, which alters basic brain synaptic and circuit function. This in turn generates the behavioral anomalies which constitute IDD. The study of patients and animal models brings the genetic components into sharp focus, and the NNC facilitates examination into how these genetic changes alter brain function and, subsequently, whether such changes are correlated to behavior. Thus, the goal of the Core is to quantify the changes in function of neuronal circuits which result from genetic mutation and variation. Elucidating the changes in dynamic function of neuronal circuitry responsible for the generation of the atypical behaviors collectively termed the IDD is critical in translational efforts to provide diagnostic and prognostic biological markers (biomarkers) as well as biologically-based indices to serve as stratification biomarkers for clinical trial enrichment and response monitoring with the ultimate goal of developing better therapies to treat and cure brain pathologies underlying the IDDs and other related CNS disorders such as autism, epilepsy, and cerebral palsy. Eligibility: These services are available both to approved users of the IDDRC at CHOP/UPenn and to users at other Centers in the nationwide Network.

(核心E-NNC：神经成像和神经电路核心) 项目总结 描述：神经成像和神经回路核心(NNC)使研究大脑回路的变化成为可能 这发生在患有IDD的人身上。核心支持患者和动物模型的研究通过提供 用户使用以下最先进的技术：(A)脑磁图(MEG)脑部记录 活动；(B)脑部结构和功能的磁共振成像(MRI)和波谱研究； (C)神经元回路动力学的多光子、共聚焦和荧光显微成像研究 功能；(D)神经元的膜片钳记录研究；和(E)脑电(EEG)和体内其他 录音。分析在受影响的人类IDD患者中进行，在IDD的临床前动物模型中， 和体外(干细胞和组织培养)。核心控制器为用户提供了广泛的访问权限 难以获得的神经回路评估技术，以及关于实验的专家咨询 设计和分析。在所有核心职能中，重点放在质量控制上。除了确保所有人 能够胜任地进行研究并进行适当的分析，核心主任协助用户设计研究 控制得当，动力充足，并且在可行的情况下，以盲目的方式进行。 与IDDRC任务的相关性：该IDDRC的总体主题是“基因、大脑和行为”(见 概述)。本核心侧重于这些重叠领域中的每一个。基因改变是许多IDD的基础， 并导致蛋白表达异常，从而改变基本的大脑突触和回路功能。这又反过来 生成构成IDD的行为异常。对患者和动物模型的研究带来了 基因成分的敏锐焦点，NNC促进了对这些基因变化如何改变的检查 大脑功能，以及随后这些变化是否与行为相关。因此，核心的目标是 就是量化基因突变和变异导致的神经元回路功能的变化。 阐明非典型性神经节细胞发生神经回路的动态功能变化 统称为IDD的行为在提供诊断和预后的翻译工作中至关重要 生物标记物(生物标记物)以及作为分层生物标记物的基于生物的指数 临床试验丰富和反应监测，最终目标是开发更好的治疗方法 治疗IDDS的大脑病理和其他相关的中枢神经系统疾病，如自闭症、癫痫和 脑性瘫痪。 资格：这些服务既向位于CHOP/UPenn的IDDRC的批准用户提供，也向位于 全国网络中的其他中心。