Neuroimaging & Neurocircuitry Core

神经影像学

• 批准号: 10678901
• 负责人: Timothy P Roberts
• 金额: $ 18.16万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10678901
• 关键词: Achievement; Address; Affect; Ally; Animal Model; Basal Ganglia; Behavior; Behavior assessment; Behavioral; Biological Markers; Biometry; Blinded; Brain; Brain Pathology; Brain imaging; Calcium; Cells; Central Nervous System; Central Nervous System Diseases; Cerebral Palsy; Clinical Trials; Collection; Consult; Consultations; Country; Coupled; DNA Sequence Alteration; Data; Data Science; Data Set; Databases; Development; Developmental Disabilities; Diagnostic; Disease; Dyes; Electroencephalography; Eligibility Determination; Epilepsy; Experimental Designs; Functional Imaging; Functional Magnetic Resonance Imaging; Functional disorder; Generations; Genes; Genetic; Genetic Variation; Goals; Growth; Human; Image; In Vitro; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Investigation; Limbic System; Magnetic Resonance Imaging; Magnetoencephalography; Mediating; Meta-Analysis; Methodology; Methods; Mission; Modality; Modeling; Monitor; Mus; Mutation; Neocortex; Neurons; Neurosciences; Patients; Persons; Physiology; Population; Pre-Clinical Model; Procedures; Protocols documentation; Quality Control; Research; Research Design; Research Personnel; Research Support; Series; Services; Slice; Spectrum Analysis; Statistical Data Interpretation; Stratification; Structure; Symptoms; Synapses; Techniques; Technology; Tissues; Training; autism spectrum disorder; brain circuitry; cognitive function; data sharing; experimental analysis; human model; imaging study; improved; in vivo; indexing; induced pluripotent stem cell; member; microscopic imaging; multi-photon; multimodality; multiphoton microscopy; neocortical; neural; neural circuit; neurochemistry; neuroimaging; neuronal circuitry; neurophysiology; novel; novel strategies; operation; patch clamp; power analysis; pre-clinical; prognostic; programs; protein expression; response; stem cells; structural imaging; temporal measurement; tissue culture; translational approach; translational therapeutics; voltage

(CORE E- NNC: NEUROIMAGING AND NEUROCIRCUITRY CORE) PROJECT SUMMARY Description: The Neuroimaging and Neurocircuitry Core (NNC) enables the study of changes in brain circuitry which occur in people with IDD. The Core supports research in both patients and animal models by providing users the following state-of-the-art technologies: (a) Magnetoencephalographic (MEG) recordings of brain activity; (b) Magnetic resonance imaging (MRI) and spectroscopy (MRS) studies of brain structure and function; (c) Multiphoton, confocal, and epifluorescence microscopic imaging studies of the dynamics of neuronal circuit function; (d) Patch clamp recording studies in neurons; and (e) Electroencephalographic (EEG) and other in vivo recordings. Analyses are performed in affected human patients with IDD, in Preclinical animal models of IDD, and in vitro (stem cells and tissue culture). Core Directors provide users with access to a wide set of otherwise inaccessible neuronal circuit assessment techniques, as well as expert consultation regarding experimental design and analysis. In all core functions, emphasis is placed upon quality control. In addition to assuring that all studies are competently conducted and appropriately analyzed, the Core Directors assist users to design studies that are properly controlled, adequately powered, and, when feasible, conducted in a blinded fashion. Relevance to IDDRC Mission: The overarching theme of this IDDRC is “Genes, Brain & Behavior” (see Overview). This Core focuses on each of these overlapping domains. Genetic alterations underlie many IDDs, and result in aberrant protein expression, which alters basic brain synaptic and circuit function. This in turn generates the behavioral anomalies which constitute IDD. The study of patients and animal models brings the genetic components into sharp focus, and the NNC facilitates examination into how these genetic changes alter brain function and, subsequently, whether such changes are correlated to behavior. Thus, the goal of the Core is to quantify the changes in function of neuronal circuits which result from genetic mutation and variation. Elucidating the changes in dynamic function of neuronal circuitry responsible for the generation of the atypical behaviors collectively termed the IDD is critical in translational efforts to provide diagnostic and prognostic biological markers (biomarkers) as well as biologically-based indices to serve as stratification biomarkers for clinical trial enrichment and response monitoring with the ultimate goal of developing better therapies to treat and cure brain pathologies underlying the IDDs and other related CNS disorders such as autism, epilepsy, and cerebral palsy. Eligibility: These services are available both to approved users of the IDDRC at CHOP/UPenn and to users at other Centers in the nationwide Network.

（核心 E-NNC：神经影像和神经回路核心） 项目概要 描述：神经影像和神经电路核心 (NNC) 能够研究大脑电路的变化 发生在 IDD 患者中。核心通过提供支持患者和动物模型的研究 用户可以使用以下最先进的技术： (a) 脑磁图 (MEG) 记录 活动; (b) 大脑结构和功能的磁共振成像（MRI）和光谱学（MRS）研究； (c) 神经元回路动力学的多光子、共焦和落射荧光显微成像研究 功能; (d) 神经元膜片钳记录研究； (e) 脑电图 (EEG) 和其他体内 录音。在 IDD 临床前动物模型中对受影响的 IDD 人类患者进行分析， 和体外（干细胞和组织培养）。核心董事为用户提供了访问广泛的其他资源的权限 难以接近的神经元回路评估技术，以及有关实验的专家咨询 设计和分析。在所有核心职能中，重点都放在质量控制上。除了确保所有 研究进行得当并进行适当分析，核心董事协助用户设计研究 这些都得到适当的控制、充足的动力，并且在可行的情况下以盲法方式进行。 与 IDDRC 使命的相关性：本次 IDDRC 的首要主题是“基因、大脑和行为”（参见 概述）。该核心重点关注这些重叠领域中的每一个。基因改变是许多 IDD 的基础， 并导致异常的蛋白质表达，从而改变基本的大脑突触和回路功能。这反过来 产生构成 IDD 的行为异常。对患者和动物模型的研究带来了 遗传成分成为人们关注的焦点，NNC 有助于检查这些遗传变化如何改变 大脑功能，以及随后的这些变化是否与行为相关。因此，核心的目标 是量化由基因突变和变异引起的神经元回路功能的变化。 阐明负责非典型细胞生成的神经元回路动态功能的变化 统称为 IDD 的行为对于提供诊断和预后的转化工作至关重要 生物标记（生物标记）以及基于生物学的指数作为分层生物标记 临床试验丰富和反应监测，最终目标是开发更好的治疗方法 并治愈 IDD 和其他相关中枢神经系统疾病（如自闭症、癫痫症和 脑瘫。 资格：这些服务既适用于 CHOP/UPenn 的 IDDRC 批准用户，也适用于以下机构的用户： 全国网络中的其他中心。