Core F: Systems Biology & Biostatistics

核心 F：系统生物学

• 批准号: 10615828
• 负责人: Mark James Cameron
• 金额: $ 30.22万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10615828
• 关键词: Acquired Immunodeficiency Syndrome; Aftercare; Animal Model; Animals; Area; Basic Science; Bayesian Analysis; Big Data; Bioinformatics; Biological Assay; Biological Markers; Biometry; Biostatistics Core; Blood; Cells; Clinical; Clinical Research; Clinical Sciences; Clinical Trials; Collaborations; Collection; Complex; Computer Analysis; Consultations; Data; Databases; Development; Disease Outcome; Disease Progression; Experimental Designs; Fostering; Foundations; Gender; Gene Expression Profile; Genetic Transcription; Genomics; HIV; HIV Infections; Human; Image; Immune; Immune System Diseases; Immune response; Immunological Models; Immunologics; Immunology; Immunology procedure; Immunotherapeutic agent; Immunotherapy; Inflammation; Manuscripts; Maps; Metadata; Methodology; Mission; Molecular; Molecular Analysis; Outcome; Pathogenesis; Pathogenicity; Pathway interactions; Patients; Preparation; Prevention; Process; Protein Analysis; Protocols documentation; Publications; RNA analysis; Reporting; Reproducibility; Research; Research Design; Research Personnel; Sampling; Services; Shapes; Statistical Data Interpretation; Structure; Support System; Surveys; Systems Biology; Technology; Tissue Model; Tissues; Training; Training and Education; Translational Research; Translations; Virus; Writing; advanced system; cohort; data acquisition; data dissemination; data management; data standards; deep sequencing; design; design verification; dysbiosis; fundamental research; high throughput analysis; immune function; immune reconstitution; innovation; interest; microbial; microbiome; microbiome research; microbiota; multidisciplinary; multiple omics; new technology; novel; public database; research study; sex; technology platform; tool; transcriptome sequencing; transcriptomics; translational study; transmission process; treatment response

PROJECT SUMMARY/ABSTRACT –SYSTEMS BIOLOGY & BIOSTATISTICS CORE F The RUSTBELT CFAR Systems Biology & Biostatistics Core F provides support to a wide range of CFAR investigators by offering new multi-omic technologies, advanced biostatistics and computational analysis of “big data,” a stronger foundation in functional immunology studies, and a comprehensive ‘omic data management and training platform. The key mission of Core F is to provide systems biology approaches that allow for integration of different layers of host immune responses, from molecular triggers of immune responses to transcriptional signatures and interaction with resident microbiota. We will continue to support functional immunology studies that remain fundamental to HIV research. The Core assists investigators with research rigor and reproducibility through implementation of best practices for study design, analysis, and public sharing of results. In Aim 1 Core F will support genomics and microbiome research in HIV immunology and pathogenesis by providing users with a robust technological platform for transcriptional analysis by RNA-seq as well as deep sequencing in clinical and animal models of HIV infection. In Aim 2 Core F provides training and consultation in functional immunology as well as centralized access to state-of-the-art immunologic technologies, protocols, and training for the isolation, manipulation, culturing, and expansion of primary immune cells from blood and tissue, thereby supporting the RUSTBELT CFAR’s HIV transmission, gender research, cure, and pathogenesis translation objectives. In Aim 3 Core F facilitates all aspects of systems biology studies, by offering a centralized data acquisition, management, and reporting structure for all cohorts and ‘omics, using high-capacity assay and analysis workflows as well as publicly available ‘omic data to build publications and databases. Lastly, in Aim 4 This service function includes the collection and preparation of ‘omic and functional data for coordination and distribution to investigators, collecting associated de-identified patient annotations needed for analysis, and study publication. Core F Core F performs biostatistical correlate analysis to identify key drivers of protective immune responses, assist in designing validation strategies and writing manuscripts, and provide the metadata and data standardization pipelines for upload of results to public databases. By providing fundamental immune modeling support paired with multi-omic methodologies to identify key drivers or biomarkers of the host-virus interface in HIV, Core F will foster the complete systems biology approach necessary to support CFAR investigators in their translational research toward HIV cure, prevention, gender discrepancies, response to treatment, and the immunopathological landscape.

项目摘要/摘要-系统生物学与生物统计学核心F 锈带CFAR系统生物学和生物统计学核心F为各种CFAR提供支持 研究人员通过提供新的多组学技术、先进的生物统计学和计算分析 数据，“在功能免疫学研究中有更强的基础，以及全面的基因组数据管理 和培训平台。核心F的主要任务是提供系统生物学方法，以允许 整合不同层次的宿主免疫反应，从免疫反应的分子触发到 转录签名和与驻留微生物区系的相互作用。我们将继续支持功能 免疫学研究仍然是艾滋病毒研究的基础。核心协助调查人员进行严谨的研究 通过实施研究设计、分析和公开共享的最佳实践 结果。在目标1中，核心F将支持在艾滋病毒免疫学和发病机制方面的基因组学和微生物组研究 通过为用户提供用于RNA-seq转录分析的强大技术平台 HIV感染的临床和动物模型中的测序。在AIM 2中，核心F提供以下方面的培训和咨询 功能免疫学以及集中访问最先进的免疫技术、方案和 从血液和组织中分离、操作、培养和扩增初级免疫细胞的培训， 从而支持锈带CFAR的艾滋病毒传播、性别研究、治疗和发病机制 翻译目标。在AIM 3Core F中，通过提供集中式的 所有队列和组学的数据获取、管理和报告结构，使用高容量分析和 分析工作流程以及公开可用的学术数据，以建立出版物和数据库。最后，在目标4中 这项服务职能包括收集和准备组织和功能数据，以便协调和 分发给调查人员，收集分析和研究所需的关联的未识别的患者注释 出版。核心F核心F执行生物统计相关性分析，以确定保护性免疫的关键驱动因素 答复，协助设计验证战略和撰写手稿，并提供元数据和数据 将结果上传到公共数据库的标准化管道。通过提供基本的免疫建模 支持与多种组学方法相结合，以确定主机-病毒界面的关键驱动因素或生物标志物 艾滋病毒，核心F将促进完整的系统生物学方法，以支持CFAR调查人员在他们的 艾滋病毒治疗、预防、性别差异、治疗反应和 免疫病理图景。