Cincinnati Cooperative Center of Excellence in Hematology

辛辛那提血液学卓越合作中心

• 批准号: 10458590
• 负责人: Daniel Starczynowski
• 金额: $ 85.21万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10458590
• 关键词: Affect; Area; Basic Science; Behavior; Biological Process; Biology; Blood Cells; Bone Marrow Transplantation; Bone marrow failure; Cell Therapy; Cells; Cellular biology; Clinical; Clinical Research; Clinical Trials; Clinical and Translational Science Awards; Communities; Coupled; Disease; Dysmyelopoietic Syndromes; Education; Environment; Erythrocytes; Erythroid; Experimental Hematology; Faculty; Fostering; Funding; Future; Gene Delivery; Gene Expression; Genes; Genetic; Genomics; Goals; Grant; Growth; Hematological Disease; Hematologist; Hematology; Hematopoiesis; Hematopoietic; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Hemoglobinopathies; Home; Image; Immunology; In Vitro; Infrastructure; Inherited; International; Lentivirus; Life; Link; Lymphoid; Medical center; Medicine; Mentors; Methods; Molecular; Molecular Biology; Mouse Strains; Mus; Myelogenous; Non-Malignant; Office of Administrative Management; Pediatric Hospitals; Plant Roots; Production; Publications; Recording of previous events; Research; Research Activity; Research Personnel; Retroviridae; Scientist; Services; Sickle Cell Anemia; Signal Transduction; Sorting - Cell Movement; Structure; Students; Technology; Therapeutic; Time; Training; Transgenic Organisms; Translational Research; Transplantation; United States National Institutes of Health; Universities; Viral; Viral Vector; Virus; base; bone marrow failure syndrome; career; cell behavior; clinical care; collaborative approach; collaborative environment; college; data exchange; drug discovery; educational atmosphere; gene therapy; genome editing; immunohematology; in vivo; innovation; interdisciplinary approach; member; molecular hematology; nature center; outreach; pre-clinical; pre-clinical research; programs; recruit; screening; small hairpin RNA; social media; stem cell biology; symposium; technology development; translational applications; translational study; translational therapeutics; vector

SUMMARY/Abstract: The long-term goal of the Cincinnati Cooperative Center of Excellence in Hematology (CCCEH) is to understand and correct, at the molecular level, hematological diseases of various lineages. To attain this goal, we must understand basic biological processes that affect behaviors of hematopoietic stem cells and their various differentiated progenies in vitro and in vivo. We envision this long-term goal will be best accomplished by interdisciplinary and collaborative approaches using state-of-the-art methods, along with timely and rational use of translational studies into the preclinical and clinical settings. Cincinnati Children's Hospital Medical Center (CCHMC) has developed a rich and stimulating intellectual environment that brings together outstanding expertise and basic research in stem cell biology, virus vector technology, immunohematologic cell biology, genomics and genetics, and hematopoiesis; in addition, the CCCEH has world-class expertise in translational and clinical research in hematology, immunology and hematopoietic stem cell transplantation. This focus has been greatly strengthened by the recruitment of over 30 faculty members in these areas of hematology research over the last 15 years at CCHMC, and the inclusion of over 15 external hematology researchers around US. Basic research is linked with Cincinnati NIH-funded Clinical and Translational Science Award (CTSA), which has facilitated interdisciplinary interactions and developed an infrastructure that supports state-of-the-art clinical trials in cell and gene therapy. Here we seek to solidify the exciting and systemic growth of research in hematology at CCHMC to support a national center of excellence, using the shared services and administrative structure of the center grant mechanism. The CCCEH Administrative Core supports an exceptional array of research cores, which function together to support innovative basic and translational research both locally and nationally, in non-malignant hematology. Our three proposed research cores include (1) Xenotransplant and Genome Editing Core, which maintains specialized mouse strains and provides mouse transplant and transgenic services; (2) Single Cell Characterization and Procurement Core that includes state- of-the-art imaging as well as single cell capture and gene expression capabilities; and (3) Gene Delivery Core that covers services such as ex vivo manipulation of blood cells by lentivirus and retrovirus transduction and shRNA/sgRNA screening. Finally, this center seeks support for an innovative Enrichment Program that fosters a collaborative and educational environment in the center and for outreach to the broad hematology research community around US.

摘要/摘要： 辛辛那提血液学卓越合作中心 (CCCEH) 的长期目标是 在分子水平上理解并纠正各种谱系的血液疾病。为了实现这一目标， 我们必须了解影响造血干细胞及其行为的基本生物过程 体外和体内各种分化的后代。我们预计这一长期目标将得到最好的实现 通过跨学科和协作的方法，使用最先进的方法，以及及时和合理的 将转化研究应用于临床前和临床环境。辛辛那提儿童医院医疗 中心 (CCHMC) 创造了一个丰富且富有启发性的智力环境，汇集了 在干细胞生物学、病毒载体技术、免疫血液细胞方面拥有杰出的专业知识和基础研究 生物学、基因组学和遗传学以及造血学；此外，CCCEH 在以下领域拥有世界一流的专业知识： 血液学、免疫学和造血干细胞移植领域的转化和临床研究。 通过在这些领域招聘 30 多名教员，这一重点得到了极大的加强。 CCHMC 过去 15 年的血液学研究，以及超过 15 项外部血液学研究 美国各地的研究人员。基础研究与辛辛那提 NIH 资助的临床和转化科学相关 奖（CTSA），促进了跨学科互动并开发了支持的基础设施 最先进的细胞和基因治疗临床试验。在这里，我们寻求巩固令人兴奋的系统性增长 CCHMC 的血液学研究利用共享服务和 中心补助机制的行政结构。 CCCEH 行政核心支持 一系列特殊的研究核心，它们共同支持创新的基础和转化 在本地和国家范围内进行非恶性血液学研究。我们提出的三个研究核心包括 (1) 异种移植和基因组编辑核心，维护专门的小鼠品系并提供小鼠 移植和转基因服务； (2) 单电池表征和采购核心，包括状态- 最先进的成像以及单细胞捕获和基因表达能力； (3) 基因传递核心 涵盖通过慢病毒和逆转录病毒转导对血细胞进行离体操作等服务 shRNA/sgRNA 筛选。最后，该中心寻求对创新丰富计划的支持，该计划旨在培养 中心的协作和教育环境以及广泛的血液学研究的推广 美国各地的社区。