Cincinnati Cooperative Center of Excellence in Hematology

辛辛那提血液学卓越合作中心

• 批准号: 10201885
• 负责人: Daniel Starczynowski
• 金额: $ 85.21万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10201885
• 关键词: Affect; Area; Basic Science; Behavior; Biological Process; Biology; Blood Cells; Bone Marrow Transplantation; Cell Therapy; Cells; Cellular biology; Clinical; Clinical Research; Clinical Trials; Clinical and Translational Science Awards; Communities; Coupled; Disease; Dysmyelopoietic Syndromes; Education; Environment; Erythrocytes; Erythroid; Experimental Hematology; Faculty; Fostering; Funding; Future; Gene Delivery; Gene Expression; Genes; Genetic; Genomics; Goals; Grant; Growth; Hematological Disease; Hematologist; Hematology; Hematopoiesis; Hematopoietic; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Hemoglobinopathies; Home; Image; Immunology; In Vitro; Infrastructure; Inherited; International; Lentivirus; Life; Link; Lymphoid; Medical center; Medicine; Mentors; Methods; Molecular; Molecular Biology; Mouse Strains; Mus; Myelogenous; Non-Malignant; Office of Administrative Management; Pancytopenia; Pediatric Hospitals; Plant Roots; Production; Publications; Recording of previous events; Research; Research Activity; Research Personnel; Retroviridae; Scientist; Services; Sickle Cell Anemia; Signal Transduction; Sorting - Cell Movement; Structure; Students; Technology; Therapeutic; Time; Training; Transgenic Organisms; Translational Research; Transplantation; United States National Institutes of Health; Universities; Viral; Viral Vector; Virus; base; bone marrow failure syndrome; career; cell behavior; clinical care; collaborative approach; collaborative environment; college; data exchange; drug discovery; educational atmosphere; gene therapy; genome editing; immunohematology; in vivo; innovation; interdisciplinary approach; member; molecular hematology; nature center; outreach; pre-clinical; pre-clinical research; programs; recruit; screening; small hairpin RNA; social media; stem cell biology; symposium; technology development; translational study; vector

SUMMARY/Abstract: The long-term goal of the Cincinnati Cooperative Center of Excellence in Hematology (CCCEH) is to understand and correct, at the molecular level, hematological diseases of various lineages. To attain this goal, we must understand basic biological processes that affect behaviors of hematopoietic stem cells and their various differentiated progenies in vitro and in vivo. We envision this long-term goal will be best accomplished by interdisciplinary and collaborative approaches using state-of-the-art methods, along with timely and rational use of translational studies into the preclinical and clinical settings. Cincinnati Children's Hospital Medical Center (CCHMC) has developed a rich and stimulating intellectual environment that brings together outstanding expertise and basic research in stem cell biology, virus vector technology, immunohematologic cell biology, genomics and genetics, and hematopoiesis; in addition, the CCCEH has world-class expertise in translational and clinical research in hematology, immunology and hematopoietic stem cell transplantation. This focus has been greatly strengthened by the recruitment of over 30 faculty members in these areas of hematology research over the last 15 years at CCHMC, and the inclusion of over 15 external hematology researchers around US. Basic research is linked with Cincinnati NIH-funded Clinical and Translational Science Award (CTSA), which has facilitated interdisciplinary interactions and developed an infrastructure that supports state-of-the-art clinical trials in cell and gene therapy. Here we seek to solidify the exciting and systemic growth of research in hematology at CCHMC to support a national center of excellence, using the shared services and administrative structure of the center grant mechanism. The CCCEH Administrative Core supports an exceptional array of research cores, which function together to support innovative basic and translational research both locally and nationally, in non-malignant hematology. Our three proposed research cores include (1) Xenotransplant and Genome Editing Core, which maintains specialized mouse strains and provides mouse transplant and transgenic services; (2) Single Cell Characterization and Procurement Core that includes state- of-the-art imaging as well as single cell capture and gene expression capabilities; and (3) Gene Delivery Core that covers services such as ex vivo manipulation of blood cells by lentivirus and retrovirus transduction and shRNA/sgRNA screening. Finally, this center seeks support for an innovative Enrichment Program that fosters a collaborative and educational environment in the center and for outreach to the broad hematology research community around US.

概要/摘要： 辛辛那提血液学卓越合作中心（CCCEH）的长期目标是 在分子水平上理解和纠正各种血统的血液病。为了实现这一目标， 我们必须了解影响造血干细胞行为的基本生物学过程， 在体外和体内的各种分化后代。我们设想，这一长期目标将最好地实现 通过跨学科和协作的方法，使用最先进的方法，沿着及时和合理的 在临床前和临床环境中使用转化研究。辛辛那提儿童医院 中心（CCHMC）开发了一个丰富和刺激的智力环境，汇集了 在干细胞生物学、病毒载体技术、免疫血液学细胞、 生物学、基因组学和遗传学以及造血;此外，CCCEH在以下方面拥有世界一流的专业知识： 血液学、免疫学和造血干细胞移植的转化和临床研究。 这一重点已大大加强了招聘30多名教师在这些领域， CCHMC过去15年的血液学研究，并纳入超过15个外部血液学 美国各地的研究人员。基础研究与辛辛那提国立卫生研究院资助的临床和转化科学有关 该奖项促进了跨学科的互动，并开发了一个基础设施， 最先进的细胞和基因治疗临床试验。在这里，我们寻求巩固令人兴奋的系统性增长， 在CCHMC血液学研究，以支持卓越的国家中心，使用共享服务， 中心赠款机制的行政结构。CCCEH管理核心支持 研究核心的特殊阵列，共同发挥作用，以支持创新的基础和翻译 在当地和全国范围内进行非恶性血液学研究。我们提出的三个研究核心包括 (1)异种移植和基因组编辑核心，保持专门的小鼠品系，并提供小鼠 移植和转基因服务;（2）单细胞表征和采购核心，包括国家- 最先进的成像以及单细胞捕获和基因表达能力;和（3）基因递送核心 包括通过慢病毒和逆转录病毒转导对血细胞进行离体操作等服务， shRNA/sgRNA筛选。最后，该中心寻求支持创新的丰富计划， 在中心的协作和教育环境，并推广到广泛的血液学研究 我们周围的社区