Mapping of chemosensory neuron function to uncover changes in neuronal fates
Mapping of chemosensory neuron function to uncover changes in neuronal fates
批准号:
10460530
负责人:
Ray L Hong
金额:
$36.25万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-08-02 至 2025-05-31
关键词:
AddressAdolescentAdultAffectAfferent NeuronsAnimal ModelAnimalsBehaviorBehavioralCaenorhabditis elegansCalciumCellsCellular MorphologyChemotaxisClustered Regularly Interspaced Short Palindromic RepeatsComparative StudyComplexCuesDataDefectDetectionDevelopmentEnsureExhibitsGene ExpressionGenesGeneticGenetic TranscriptionGenetic studyHealthHistamineHomologous GeneHumanHydroxysteroid DehydrogenasesIndividualInsect VectorsInsectaIonsKnock-outLarvaLife Cycle StagesMammalsMapsMediatingModelingMolecularNematodaNervous system structureNeuroanatomyNeuronal DifferentiationNeuronsOdorsParasitesParasitic nematodePathway interactionsPersonsPlayPreventive measurePreventive treatmentProcessPublishingResearchResearch SupportRoleSensorySmell PerceptionSodium ChlorideSystemTaste PerceptionTaste preferencesTestingTransgenesTranslatingZinc Fingersbasebehavior testcell typecomparativeforward geneticsgene regulatory networkgenome annotationhomeodomainimprovedin vivoknock-downmature animalmutantpreferencereproductiveresponsereverse geneticssensortranscription factortransmission processvector transmission
中文摘要
项目摘要
全球寄生线虫感染人数超过1.5亿,感染人数高达13亿
人们每年都会从预防性治疗中受益。许多寄生线虫需要
昆虫寄主,在其复杂的生活史中传播给人类寄主。我们使用一种
遗传易驯化的太平洋棱线虫,与昆虫有关,是一种
研究如何从有限的神经元集合中产生不同行为的比较模型。
我们提出的研究解决了一个基本问题,即神经系统如何
进化以适应新的行为,特别是如何以及哪些转录因子
来指定宿主中化学感觉神经元的发育命运-
寻求行为。我们将使用反向遗传学来定位候选转录
被称为末端选择器基因的因子,以及可诱导的转基因敲击
在单细胞水平上下调神经元功能。我们还将使用正向遗传学来
不偏不倚地研究哪些基因对口味偏好的改变起作用
当线虫发育成生殖成虫或寻找寄主时,它们会闻到
感染性幼虫。详细了解神经元重塑是如何影响的
化学感觉可以揭示在不同物种中发现的感觉神经元的基本蓝图
线虫,它可以提供中断寄生线虫传播的方法
在昆虫和人类宿主之间。
英文摘要
Project Summary
Parasitic nematodes infect more than 150 million people worldwide and up to 1.3 billion
people benefit from preventative treatment annually. Many parasitic nematodes require
insect hosts for transmission to human hosts in their complex life cycles. We use a
genetically tractable nematode Pristionchus pacificus that is associated with insects as a
comparative model to study how diverse behaviors arise from a limited set of neurons.
Our proposed research addresses a fundamental question on how nervous systems
evolve to accommodate new behaviors, specifically how and which transcription factors
are required to designate the developmental fates of chemosensory neurons in host-
seeking behavior. We will use reverse genetics to target candidate transcriptional
factors, known as terminal selector genes, as well as inducible transgenes to knock
down neuronal function at the single cell level. We will also use forward genetics to
investigate unbiasedly which genes are responsible to switch the preferences for taste
and smell when the nematodes develop into reproductive adults or host-seeking
infectious larvae. A detailed understanding of how neuronal remodeling affects
chemosensation could reveal the basic blueprint for sensory neurons found in diverse
nematodes, which could inform ways to disrupt the transmission of parasitic nematodes
between insect and human hosts.
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Administrative Supplements for Curriculum or Training Modules to Enhance Undergraduate Biomedical Research Training: Pre-CURE Modules In Biology
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批准号:10393442
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项目类别:
-
资助金额:$6.67万
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财政年份:2021
-
负责人:Ray L Hong
-
依托单位:
Mapping of chemosensory neuron function to uncover changes in neuronal fates
-
批准号:10636843
-
项目类别:
-
资助金额:$36.25万
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财政年份:2021
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负责人:Ray L Hong
-
依托单位:
Mapping of chemosensory neuron function to uncover changes in neuronal fates
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批准号:10171141
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项目类别:
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资助金额:$34.45万
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财政年份:2021
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负责人:Ray L Hong
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依托单位:
Elucidating the function of the Lipid-Binding Protein OBI-1 in Pristionchus pacif
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批准号:8692939
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项目类别:
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资助金额:$10.88万
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财政年份:2013
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负责人:Ray L Hong
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依托单位:
The mode-of-action for pheromone-induced paralysis in Pristionchus pacificus
-
批准号:9978066
-
项目类别:
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资助金额:$10.88万
-
财政年份:2013
-
负责人:Ray L Hong
-
依托单位:
Elucidating the function of the Lipid-Binding Protein OBI-1 in Pristionchus pacif
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批准号:9086371
-
项目类别:
-
资助金额:$10.88万
-
财政年份:2013
-
负责人:Ray L Hong
-
依托单位:
Elucidating the function of the Lipid-Binding Protein OBI-1 in Pristionchus pacif
-
批准号:8871734
-
项目类别:
-
资助金额:$10.88万
-
财政年份:2013
-
负责人:Ray L Hong
-
依托单位:
Elucidating the function of the Lipid-Binding Protein OBI-1 in Pristionchus pacif
-
批准号:8475301
-
项目类别:
-
资助金额:$10.88万
-
财政年份:2013
-
负责人:Ray L Hong
-
依托单位:
Molecular Characterization of Insect Pheromone Chemosensory Mutants in Nematodes
-
批准号:8212253
-
项目类别:
-
资助金额:$14.36万
-
财政年份:2010
-
负责人:Ray L Hong
-
依托单位:
Molecular Characterization of Insect Pheromone Chemosensory Mutants in Nematodes
-
批准号:8015243
-
项目类别:
-
资助金额:$14.36万
-
财政年份:2010
-
负责人:Ray L Hong
-
依托单位:
Molecular Characterization of Insect Pheromone Chemosensory Mutants in Nematodes
-
批准号:7761672
-
项目类别:
-
资助金额:$12.25万
-
财政年份:2010
-
负责人:Ray L Hong
-
依托单位:
海外基金