Mapping of chemosensory neuron function to uncover changes in neuronal fates

绘制化学感应神经元功能图谱以揭示神经元命运的变化

• 批准号: 10636843
• 负责人: Ray L Hong
• 金额: $ 36.25万
• 依托单位: CALIFORNIA STATE UNIVERSITY NORTHRIDGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-02 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10636843
• 关键词: Address; Adolescent; Adult; Affect; Afferent Neurons; Animals; Behavior; Behavioral; Caenorhabditis elegans; Calcium; Cells; Cellular Morphology; Chemotaxis; Clustered Regularly Interspaced Short Palindromic Repeats; Comparative Study; Complex; Cues; Data; Defect; Detection; Development; Ensure; Esthesia; Exhibits; Gene Expression; Genes; Genetic; Genetic Transcription; Genetic study; Health; Histamine; Homologous Gene; Human; Hydroxysteroid Dehydrogenases; Individual; Insect Vectors; Insecta; Ions; Knock-out; Larva; Life Cycle Stages; Mammals; Maps; Mediating; Modeling; Molecular; Nematoda; Nervous System; Neuroanatomy; Neuronal Differentiation; Neurons; Odors; Parasites; Parasitic nematode; Pathway interactions; Persons; Play; Preventive measure; Preventive treatment; Process; Publishing; Research; Research Support; Role; Sensory; Smell Perception; Sodium Chloride; System; Taste Perception; Taste preferences; Testing; Transgenes; Translating; Visualization; Zinc Fingers; behavior test; cell type; comparative; forward genetics; gene regulatory network; genome annotation; homeodomain; improved; in vivo; knock-down; mature animal; model organism; mutant; preference; reproductive; response; reverse genetics; sensor; transcription factor; transmission process; vector transmission

Project Summary Parasitic nematodes infect more than 150 million people worldwide and up to 1.3 billion people benefit from preventative treatment annually. Many parasitic nematodes require insect hosts for transmission to human hosts in their complex life cycles. We use a genetically tractable nematode Pristionchus pacificus that is associated with insects as a comparative model to study how diverse behaviors arise from a limited set of neurons. Our proposed research addresses a fundamental question on how nervous systems evolve to accommodate new behaviors, specifically how and which transcription factors are required to designate the developmental fates of chemosensory neurons in host- seeking behavior. We will use reverse genetics to target candidate transcriptional factors, known as terminal selector genes, as well as inducible transgenes to knock down neuronal function at the single cell level. We will also use forward genetics to investigate unbiasedly which genes are responsible to switch the preferences for taste and smell when the nematodes develop into reproductive adults or host-seeking infectious larvae. A detailed understanding of how neuronal remodeling affects chemosensation could reveal the basic blueprint for sensory neurons found in diverse nematodes, which could inform ways to disrupt the transmission of parasitic nematodes between insect and human hosts.

项目总结

项目成果

Evolution and Diversity of TGF-β Pathways are Linked with Novel Developmental and Behavioral Traits.

• DOI: 10.1093/molbev/msac252
• 发表时间: 2022-12-05
• 期刊: MOLECULAR BIOLOGY AND EVOLUTION
• 影响因子: 10.7
• 作者: Lo, Wen-Sui;Roca, Marianne;Dardiry, Mohannad;Mackie, Marisa;Eberhardt, Gabi;Witte, Hanh;Hong, Ray;Sommer, Ralf J.;Lightfoot, James W.
• 通讯作者: Lightfoot, James W.