Synthetic adenovirus libraries for vector optimization
用于载体优化的合成腺病毒文库
基本信息
- 批准号:10460635
- 负责人:
- 金额:$ 36.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-20 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAdenovirus VectorAdenovirusesAdoptedBar CodesBiomedical ResearchCapsid ProteinsCell physiologyCellular StressCharacteristicsComplexCosmidsCustomDNADNA LibraryDNA VirusesDNA biosynthesisDevelopmentDisciplineEpitopesFrequenciesGene DeliveryGenerationsGenesGenetic ScreeningGenetic TranscriptionGenetic VariationGenomeGenomic LibraryGoalsHumanIn VitroIndividualInfectionKnowledgeLibrariesMammalian CellMethodsMutagenesisMutationNatureNormal CellOpen Reading FramesPlasmidsProcessProductionPropertyProteinsRNA ProcessingReactionResourcesScientistSerotypingSignal PathwaySystemTechniquesTechnologyTissuesToxic effectTransgenesTropismTubeVariantViralViral GenomeViral PhysiologyVirusbasebiological adaptation to stresscell typecombinatorialdesignfield studyhomologous recombinationimprovedinnovationinterestmutantneutralizing monoclonal antibodiesnext generationnovelrecombinant adenovirussynthetic biologysynthetic constructtooltraittransgene deliveryvectorwhole genome
项目摘要
Synthetic adenovirus libraries for vector optimization
Adenoviruses have been extensively utilized to address fundamental questions in biomedical research. These
small DNA viruses rely on numerous host proteins for their own replication, and have been useful probes to
explore the fundamental workings of the mammalian cell. Genetically tractable, recombinant adenoviruses
have become widely used as vectors for gene delivery. Both rational and unbiased methods have been
employed to enhance and modify their functional characteristics. Rational approaches to vector optimization
are constrained by our incomplete understanding of many viral functions, while unbiased methods are
technically limited by the size of the genome and the inherent difficulty of mutagenizing specific genes of
interest. This proposal is focused on the development of a novel platform for the creation of customized
adenovirus libraries. The critical innovation that underlies this project is a recently developed system for the in
vitro assembly of adenovirus genomes from compact modules that can be individually manipulated and then
reassembled. In this project, synthetic methods will be employed to generate focal regions of high diversity
across the genome of human adenovirus 5, the most widely-employed vector serotype. The resulting DNA
sub-libraries will be assembled into complete viral genomes, with each assembly assigned a unique barcode
designed to facilitate library characterization and the tracking of individual mutants. The construction and
characterization of high-content, focal adenoviral libraries, and their utility, will be demonstrated by a simple
screen for mutant viruses that evade recognition by a neutralizing monoclonal antibody. The long term goal of
this project is to develop a resource that can be shared and continually developed to meet the evolving needs
of scientists across many disciplines.
用于载体优化的合成腺病毒文库
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses.
- DOI:10.1016/j.xpro.2022.101123
- 发表时间:2022-03-18
- 期刊:
- 影响因子:0
- 作者:Jang Y;Bunz F
- 通讯作者:Bunz F
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{{ truncateString('FRED BUNZ', 18)}}的其他基金
Synthetic adenovirus libraries for vector optimization
用于载体优化的合成腺病毒文库
- 批准号:
10188568 - 财政年份:2019
- 资助金额:
$ 36.84万 - 项目类别:
Synthetic adenovirus libraries for vector optimization
用于载体优化的合成腺病毒文库
- 批准号:
10021682 - 财政年份:2019
- 资助金额:
$ 36.84万 - 项目类别:
A Genetic Model for Early-Onset Breast and Colon Cancer in African Americans
非裔美国人早发乳腺癌和结肠癌的遗传模型
- 批准号:
8662394 - 财政年份:2013
- 资助金额:
$ 36.84万 - 项目类别:
A Genetic Model for Early-Onset Breast and Colon Cancer in African Americans
非裔美国人早发乳腺癌和结肠癌的遗传模型
- 批准号:
9096071 - 财政年份:2013
- 资助金额:
$ 36.84万 - 项目类别:
A Genetic Model for Early-Onset Breast and Colon Cancer in African Americans
非裔美国人早发乳腺癌和结肠癌的遗传模型
- 批准号:
8704891 - 财政年份:2013
- 资助金额:
$ 36.84万 - 项目类别:
A Genetic Model for Early-Onset Breast and Colon Cancer in African Americans
非裔美国人早发乳腺癌和结肠癌的遗传模型
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8389392 - 财政年份:2012
- 资助金额:
$ 36.84万 - 项目类别:
Targeting the ATR pathway in p53-deficient cancers
靶向 p53 缺陷癌症中的 ATR 通路
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8300806 - 财政年份:2011
- 资助金额:
$ 36.84万 - 项目类别:
Targeting the ATR pathway in p53-deficient cancers
靶向 p53 缺陷癌症中的 ATR 通路
- 批准号:
8082529 - 财政年份:2011
- 资助金额:
$ 36.84万 - 项目类别:
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