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Epigenetic Mechanisms Underlying Maternal Diabetes Associated Cardiac Malformations

孕产妇糖尿病相关心脏畸形的表观遗传机制

基本信息

批准号：
10462586
负责人：
Vidu Garg
金额：
$ 38万
依托单位：
RESEARCH INST NATIONWIDE CHILDREN'S HOSP
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10462586
关键词：
ATAC-seq Address Adult Affect Animal Model Backcrossings Binding Sites Biological Availability Blood Glucose Cardiac Cardiac development Cardiovascular system Cause of Death Cell Lineage Cells Childhood Chromatin Chromatin Remodeling Factor Chromosome abnormality Complex Congenital Abnormality Congenital Heart Defects Developmental Gene Diabetes Mellitus Embryonic Development Endothelium Environment Environmental Risk Factor Epidemiology Epigenetic Process Etiology Exposure to Family Fibrinogen Gene Dosage Gene Expression Genes Genetic Genetic Predisposition to Disease Genetic Transcription Genomic approach Goals Health Heart Heart Abnormalities Heterozygote Human Hyperglycemia In Vitro Incidence Individual Infant Knowledge Mediating Mesenchymal Modeling Molecular Morbidity - disease rate Morphogenesis Mus Mutation NF-kappa B NOTCH1 gene Nitric Oxide Nitric Oxide Signaling Pathway Nitric Oxide Synthase Notch Signaling Pathway Oxidation-Reduction Oxidative Stress Pathway interactions Polycomb Pregnancy Prevention strategy Production Proteomics Publishing Reactive Oxygen Species Regulation Regulator Genes Reporting Research Risk Risk Factors Role Signal Pathway Signal Transduction Single-Gene Defect Specificity Survivors Teratogens Ventricular septum Work cardiogenesis congenital heart disorder conotruncal heart defect diabetic embryopathy epidemiology study epigenetic regulation gene environment interaction genetic technology in vivo malformation maternal diabetes maternal hyperglycemia member notch protein novel prenatal exposure promoter response semilunar valve septal defect transcriptome sequencing

项目摘要

Abstract Congenital heart defects constitute a significant pediatric and adult health problem. They not only represent the leading non-infectious cause of death in infants, but the growing number of adult survivors also suffer significant morbidity. Numerous genetic contributors have been identified to cause congenital heart defects ranging from chromosome abnormalities to single gene defects. Congenital heart disease-causing genes have been well-studied using animal models and the molecular pathways regulating normal cardiac morphogenesis are being increasingly defined. Environmental factors are known to increase the incidence of congenital heart defects. A significant knowledge gap exists in our understanding of the mechanisms by which environmental factors affect the molecular pathways regulating heart development to increase the risk of congenital heart disease. Maternal diabetes is a well-established and increasingly prevalent environmental risk factor for congenital heart disease. We recently described a novel gene-environment interaction between maternal hyperglycemia and Notch1 signaling that increased the risk of congenital heart defects in animal models. Our studies suggested that alterations in the epigenetic regulation of the nitric oxide and Notch1 signaling pathways were responsible for this interaction. Our long-term objective is to define the molecular and cellular pathways underlying this interaction and define a paradigm by which gene-environment interactions occur to cause congenital heart defects. The overall hypothesis of this research is that maternal diabetes-associated congenital heart disease occurs when maternal hyperglycemia-induced reactive oxygen species disrupts normal cardiac morphogenesis by epigenetic mechanisms and will be addressed in the following specific aims: Aim 1. To determine the cell-specific and temporal mechanisms by which hyperglycemia-associated oxidative stress mediates reduced chromatin accessibility at the Nos3 locus to cause congenital heart disease. Aim 2. To define the mechanism by which nitric oxide regulates the expression of the epigenetic Notch1 regulator, Jarid2. Aim 3. To determine if alterations in chromatin regulatory genes that occur with hyperglycemia contribute to maternal diabetes-associated congenital heart defects. Elucidating the molecular basis for the epidemiologic association between diabetes and cardiac malformations is required in order to devise novel preventive strategies for diabetes-associated congenital heart disease and potentially identify at risk individuals. Successful completion of the proposed studies will result in a significant advancement in our molecular understanding of the mechanisms by which gene-environment interactions contribute to congenital heart defect occurrence.

摘要先天性心脏病是一个严重的儿童和成人健康问题。他们不是仅代表婴儿死亡的主要非传染性原因，但成年人的数量不断增加幸存者也遭受着严重的发病率。许多基因贡献者已经被确定为导致先天性心脏缺陷，从染色体异常到单基因缺陷。导致先天性心脏病的基因已经通过动物模型和调控正常心脏形态发生的分子途径日益明确。众所周知，环境因素会增加先天性心脏病的发生率。一个重要的知识鸿沟存在于我们对环境因素影响机制的理解中调节心脏发育的分子途径会增加先天性心脏病的风险。产妇糖尿病是一种公认的、日益普遍的环境风险因素先天性心脏病。我们最近描述了一种新的基因-环境相互作用母体高血糖和Notch1信号增加先天性心脏病的风险动物模型。我们的研究表明，一氧化氮表观遗传调控的变化 Notch1信号通路负责这种相互作用。我们的长期目标是定义这种相互作用背后的分子和细胞途径，并定义一个范例，通过它基因与环境的相互作用会导致先天性心脏缺陷。对此的总体假设是研究表明，产妇糖尿病相关的先天性心脏病发生在产妇高血糖诱导的活性氧通过以下途径破坏正常的心脏形态发生表观遗传机制，并将在以下具体目标中加以解决：目标1.确定高血糖相关氧化应激介导的细胞特异性和时间机制 NOS3基因座染色质可及性降低导致先天性心脏病。目标2.定义一氧化氮调节表观遗传Notch1调节子表达的机制， Jarid2.目的3.确定高血糖时染色质调节基因是否发生改变导致产妇糖尿病相关的先天性心脏病。阐明分子基础因为糖尿病和心脏畸形之间的流行病学联系是必要的，以便设计新的预防糖尿病相关先天性心脏病的策略，并有可能识别处于危险中的个人。成功完成拟议的研究将带来重大的基因-环境相互作用机制的分子理解研究进展相互作用导致先天性心脏缺陷的发生。