IDENTIFICATION AND MOLECULAR BASIS FOR EFFICIENT ANTIFREEZE PROTEIN ENHANCERS

高效抗冻蛋白增强剂的鉴定和分子基础

• 批准号: 10466921
• 负责人: Xin Wen
• 金额: $ 10.95万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10466921
• 关键词: Address; Affect; Affinity; Alcohol dehydrogenase; Alcohols; Antifreeze; Antifreeze Proteins; Attention; Bacteria; Binding; Biochemical; Biological; Biological Assay; Biological Products; Biophysics; Body Fluids; Bovine Serum Albumin; Cells; Cities; Collaborations; Conflict (Psychology); Creatine Kinase; Cryopreservation; Cryosurgery; Crystallization; Depressed mood; Development; Differential Scanning Calorimetry; Enhancers; Environment; Enzymes; Exhibits; Fishes; Freezing; Frozen Foods; Goals; Growth; Health; High Pressure Liquid Chromatography; Human; Hydration status; Hydrogen Bonding; Hydrophobic Interactions; Hydrophobicity; Hyperactivity; Ice; Industrialization; Insecta; Lead; Link; Measures; Methods; Molecular; Order Coleoptera; Organ; Organism; Plants; Proteins; Reporting; Research; Rest; Scientist; Series; Site-Directed Mutagenesis; Sodium Chloride; Surface; System; Testing; Time; Tissues; Translating; Water; Work; base; biological systems; biophysical techniques; carbonate dehydratase; enzyme activity; food quality; fungus; improved; melting; molecular mass; novel; novel strategies; preservation; protective effect; protective efficacy; small molecule; solute

Abstract/Summary Antifreeze proteins (AFPs) are natural antifreeze molecules that have been found in many organisms including fish, insects, plants, bacteria, and fungi. AFPs exhibit great structural diversity, while they are all characterized by their unique ability to depress the freezing point of water without affecting the melting point apparently. The resulting difference between the melting point and the freezing point, referred to as thermal hysteresis (TH), is generally used as a measure of the antifreeze activities of AFPs. AFPs and co-solutes in body-fluids of cold-adapted organisms are responsible for survival in cold environments. Certain small molecule and protein co-solutes can further enhance the antifreeze activity of AFPs, referred to as enhancers, by binding to AFP through ionic interactions, hydrogen bonding, and/or hydrophobic interactions. AFP-based antifreeze systems (AFP plus enhancer) are much more effective and their uses are more environmentally friendly in comparison to conventional antifreezes, making them intriguing alternatives to conventional antifreezes in particular in biomedical fields. The proposed research will investigate the little known AFP- protein systems through determining the cryoprotective effect of an AFP on other proteins as well as the effect of these proteins on the antifreeze activity of the AFP using combined biochemical and biophysical methods. The information on such interplay in the AFP-protein systems would not only further our understanding of biological antifreeze system in cold-adapted organisms, but also lead to the development of effective protective systems for long-term preservation of important biologics in biomedical applications.

摘要/总结 抗冻蛋白（AFP）是在许多生物体中发现的天然抗冻分子 包括鱼类、昆虫、植物、细菌和真菌。 AFP 表现出巨大的结构多样性，同时它们都是 其特点是具有降低水的冰点而不影响熔点的独特能力 显然。由此产生的熔点和凝固点之间的差异称为热 滞后（TH）通常用作AFP抗冻活性的衡量标准。 AFP 和共溶质 适应寒冷的生物体的体液负责在寒冷环境中生存。某些小 分子和蛋白质共溶质可以进一步增强AFPs的抗冻活性，称为增强剂， 通过离子相互作用、氢键和/或疏水相互作用与AFP结合。基于法新社 防冻系统（AFP 加增强剂）更加有效，并且其使用也更加环保 与传统防冻剂相比更加友好，使其成为传统防冻剂的有趣替代品 防冻剂，特别是在生物医学领域。拟议的研究将调查鲜为人知的法新社- 通过确定 AFP 对其他蛋白质的冷冻保护作用以及该作用来研究蛋白质系统 使用生化和生物物理相结合的方法研究这些蛋白质对 AFP 抗冻活性的影响。 有关 AFP-蛋白质系统中这种相互作用的信息不仅会进一步加深我们对 生物防冻系统在适应寒冷的生物体中的应用，也导致了有效防护措施的发展 用于长期保存生物医学应用中重要生物制剂的系统。