IDENTIFICATION AND MOLECULAR BASIS FOR EFFICIENT ANTIFREEZE PROTEIN ENHANCERS

高效抗冻蛋白增强剂的鉴定和分子基础

• 批准号: 10684868
• 负责人: Xin Wen
• 金额: $ 10.95万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10684868
• 关键词: Address; Affect; Affinity; Alcohol dehydrogenase; Alcohols; Antifreeze; Antifreeze Proteins; Attention; Bacteria; Binding; Biochemical; Biological; Biological Assay; Biological Preservation; Biological Products; Biophysics; Body Fluids; Bovine Serum Albumin; Cells; Cities; Collaborations; Creatine Kinase; Cryopreservation; Cryosurgery; Depressed mood; Development; Differential Scanning Calorimetry; Enhancers; Environment; Enzymes; Exhibits; Fishes; Freezing; Friends; Frozen Foods; Goals; Growth; Health; High Pressure Liquid Chromatography; Human; Hydration status; Hydrogen Bonding; Hydrophobic Interactions; Hydrophobicity; Hyperactivity; Ice; Industrialization; Insecta; Link; Measures; Methods; Molecular; Order Coleoptera; Organ; Organism; Plants; Proteins; Reporting; Research; Rest; Scientist; Series; Site-Directed Mutagenesis; Sodium Chloride; Surface; System; Testing; Tissues; Translating; Water; Work; biological systems; biophysical techniques; carbonate dehydratase; conflict resolution; enzyme activity; food quality; fungus; improved; melting; molecular mass; novel; novel strategies; preservation; protective effect; protective efficacy; small molecule

Abstract/Summary Antifreeze proteins (AFPs) are natural antifreeze molecules that have been found in many organisms including fish, insects, plants, bacteria, and fungi. AFPs exhibit great structural diversity, while they are all characterized by their unique ability to depress the freezing point of water without affecting the melting point apparently. The resulting difference between the melting point and the freezing point, referred to as thermal hysteresis (TH), is generally used as a measure of the antifreeze activities of AFPs. AFPs and co-solutes in body-fluids of cold-adapted organisms are responsible for survival in cold environments. Certain small molecule and protein co-solutes can further enhance the antifreeze activity of AFPs, referred to as enhancers, by binding to AFP through ionic interactions, hydrogen bonding, and/or hydrophobic interactions. AFP-based antifreeze systems (AFP plus enhancer) are much more effective and their uses are more environmentally friendly in comparison to conventional antifreezes, making them intriguing alternatives to conventional antifreezes in particular in biomedical fields. The proposed research will investigate the little known AFP- protein systems through determining the cryoprotective effect of an AFP on other proteins as well as the effect of these proteins on the antifreeze activity of the AFP using combined biochemical and biophysical methods. The information on such interplay in the AFP-protein systems would not only further our understanding of biological antifreeze system in cold-adapted organisms, but also lead to the development of effective protective systems for long-term preservation of important biologics in biomedical applications.

抽象/总结

项目成果

Protein/Ice Interaction: High-Resolution Synchrotron X-ray Diffraction Differentiates Pharmaceutical Proteins from Lysozyme.

• DOI: 10.1021/acs.jpcb.9b02443
• 发表时间: 2019-07-11
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY B
• 影响因子: 3.3
• 作者: Bhatnagar, Bakul;Zakharov, Boris;Fisyuk, Alexander;Wen, Xin;Karim, Fawziya;Lee, Kimberly;Seryotkin, Yurii;Mogodi, Mashikoane;Fitch, Andy;Boldyreva, Elena;Kostyuchenko, Anastasia;Shalaev, Evgenyi
• 通讯作者: Shalaev, Evgenyi

Interaction of reduced nicotinamide adenine dinucleotide with an antifreeze protein from Dendroides canadensis: mechanistic implication of antifreeze activity enhancement.

• DOI: 10.1002/jmr.1151
• 发表时间: 2011-11
• 期刊: Journal of molecular recognition : JMR
• 作者: Wen X;Wang S;Amornwittawat N;Houghton EA;Sacco MA
• 通讯作者: Sacco MA

Thermodynamic Analysis of Thermal Hysteresis: Mechanistic Insights into Biological Antifreezes.

热滞后的热力学分析：生物防冻剂的机理见解。

• DOI: 10.1016/j.jct.2012.04.028
• 发表时间: 2012
• 期刊: The Journal of chemical thermodynamics
• 作者: Wang,Sen;Amornwittawat,Natapol;Wen,Xin
• 通讯作者: Wen,Xin

Molecular recognition of methyl α-D-mannopyranoside by antifreeze (glyco)proteins.

• DOI: 10.1021/ja502837t
• 发表时间: 2014-06-25
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Wang, Sen;Wen, Xin;DeVries, Arthur L.;Bagdagulyan, Yelena;Morita, Alexander;Golen, James A.;Duman, John G.;Rheingold, Arnold L.
• 通讯作者: Rheingold, Arnold L.

Expanding the molecular recognition repertoire of antifreeze polypeptides: effects on nucleoside crystal growth.

• DOI: 10.1039/c2cc36264c
• 发表时间: 2012-12-07
• 期刊: Chemical communications (Cambridge, England)
• 作者: Wang S;Wen X;Nikolovski P;Juwita V;Arifin JF
• 通讯作者: Arifin JF