Development of Malaria Transmission-Blocking Drugs

疟疾传播阻断药物的开发

• 批准号: 10469237
• 负责人: Philip Sanderson
• 金额: $ 107.1万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10469237
• 关键词: Affect; Africa South of the Sahara; Antimalarials; Biological Assay; Bite; Chills; Culicidae; Data; Development; Diarrhea; Disease; Dose; Drug Kinetics; Evaluation; FRAP1 gene; Fever; Goals; Headache; Infection prevention; Lead; Malaria; Modeling; Myalgia; Organ; Parasite Control; Parasites; Parasitic Diseases; Patients; Persons; Pharmaceutical Preparations; Pilot Projects; Plasmodium falciparum; Recovery; Research Personnel; Rodent; Scientist; Series; Sweating; Symptoms; Testing; Time; Vomiting; Work; analog; design; in vivo; lead optimization; malaria transmission; mosquito-borne; novel drug class; reduce symptoms; transmission process

Even while on current therapies, malaria patients remain infectious for a period of time, allowing further mosquito-borne transmission to others. Control of parasite transmission is critical for elimination and eradication of malaria. However, most antimalarial drugs are not active against sexual stage P. falciparum parasites called gametocytes which are responsible for the spread of malaria from person to person via mosquitoes. To begin to fill this void, investigators screened 5,215 known bioactive compounds and approved drugs for gametocytocidal activity. One compound with favorable pharmacokinetics (Torin2) was selected as the first candidate for further evaluation, including testing in an in vivo rodent malaria transmission model. Two 4 mg/kg doses completely blocked parasites ability to infect mosquitoes, and a 2 mg/kg dose gave a partial blockade, confirming the transmission-blocking activity of Torin2. Preliminary data indicate that the Torin2 target in P. falciparum is distinct from the mammalian target, allowing the design of malaria-specific derivatives. Pilot studies between the lead collaborator and NCATS scientists used a gametocyte viability assay, a cellular mTOR assay and an in vivo rodent malaria transmission model to identify new malaria-specific Torin2 analogues. This work resulted in identification of a series of compounds suitable for lead optimization. The project currently is in late-stage lead optimization, with the goal of identifying a compound with single-dose activity against all stages of the disease.

即使在目前的治疗中，疟疾患者在一段时间内仍然具有传染性，从而允许进一步通过蚊子传播给他人。控制寄生虫传播对消灭和根除疟疾至关重要。然而，大多数抗疟疾药物对称为配子体的性阶段恶性疟原虫寄生虫没有活性，配子体是疟疾通过蚊子在人与人之间传播的原因。 为了开始填补这一空白，研究人员筛选了5,215种已知的生物活性化合物和批准的杀配子活性药物。选择一种具有良好药代动力学的化合物（Torin 2）作为进一步评估的第一候选物，包括在体内啮齿动物疟疾传播模型中进行测试。两个4 mg/kg剂量完全阻断了寄生虫感染蚊子的能力，2 mg/kg剂量产生了部分阻断，证实了Torin 2的传播阻断活性。 初步数据表明，恶性疟原虫中的Torin 2靶标与哺乳动物靶标不同，允许设计疟疾特异性衍生物。主要合作者和NCATS科学家之间的试点研究使用配子母细胞活力测定，细胞mTOR测定和体内啮齿动物疟疾传播模型来鉴定新的疟疾特异性Torin 2类似物。这项工作导致了一系列化合物的鉴定适合铅优化。该项目目前处于后期先导优化阶段，目标是确定一种对疾病所有阶段具有单剂量活性的化合物。