Strategies for Quantifying and Controlling Free Living Nematode Populations and Consequent Damage by Tobacco Rattle Virus to Improve Potato Yield and Quality

量化和控制自由生活线虫种群以及烟草脆响病毒造成的损害以提高马铃薯产量和质量的策略

• 批准号: 100876
• 金额: $ 86.24万
• 依托单位: CYGNET POTATO BREEDERS LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=100876
• 关键词: Strategies; Quantifying; Controlling; Free; Living

Free Living Nematodes (FLN) are emerging as a major problem for UK potato growers, exacerbated in the short term by removal of approved nematicides and in the long-term by expected population increases due to climate change. FLN cause direct damage by feeding on potato roots reducing yields and quality, and indirectly by transmitting Tobacco Rattle Virus (TRV). Relatively low levels of TRV infections can render entire crops unsaleable, both for the fresh and the processing industries. Current knowledge estimates the total loss to the UK potato industry to be >£13m p.a. FLN comprise a range of different taxonomic groups that are difficult to distinguish visually but vary significantly in terms of their distribution, pathogenicity and virus transmission frequencies, and have been to date under-studied in the UK. The problem is further compounded by beneficial and pathogenic FLN species co-existing, and thus accurate discrimination is essential. This project brings together a consortium of companies with a grower base of over 500 growers invloved in ware potato production seed both for use in the UK and export. In addition, a number of companies with potential methods for controlling FLN populations are included as partners. For the first time, a molecular diagnostic capable of distinguishing between the three main groups of FLN of interest will be developed, validated and deployed. This will be used to assess direct FLN feeding damage on a selection of commercial potato varieties as well as study effects on tuber quality and transmission of virus. In parallel, molecular markers will be developed to facilitate the breeding of new potato varieties with resistance to TRV.

自由生活线虫（FLN）正在成为英国马铃薯种植者面临的一个主要问题，在短期内，由于批准的杀线虫剂的取消，而在长期内，由于气候变化导致的预期人口增加，该问题会加剧。 FLN 以马铃薯根部为食，导致产量和质量下降，造成直接损害，并通过传播烟草脆裂病毒 (TRV) 造成间接损害。相对较低水平的 TRV 感染可能会导致整个农作物无法销售，无论是新鲜行业还是加工行业。目前的知识估计英国马铃薯产业每年的总损失超过 1300 万英镑。 FLN 由一系列不同的分类群组成，这些分类群在视觉上很难区分，但在分布、致病性和病毒传播频率方面差异很大，迄今为止在英国尚未得到充分研究。有益和致病 FLN 物种共存进一步加剧了问题，因此准确区分至关重要。该项目汇集了一个由超过 500 名种植者组成的公司财团，这些种植者参与了商品马铃薯种子的生产，供英国使用和出口。此外，许多拥有控制 FLN 种群潜在方法的公司也被列为合作伙伴。将首次开发、验证和部署能够区分感兴趣的 FLN 三个主要组的分子诊断。这将用于评估 FLN 直接饲喂对精选商业马铃薯品种的损害，以及研究对块茎质量和病毒传播的影响。同时，将开发分子标记以促进抗 TRV 马铃薯新品种的选育。