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Investigating the role of lipoteichoic acid in surface protein presentation

研究脂磷壁酸在表面蛋白呈递中的作用

基本信息

批准号：
10471833
负责人：
Bruno P Lima
金额：
$ 12.4万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-18 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10471833
关键词：
Adhesions Adhesives Affect Anabolism Animal Model Bacillus anthracis Bacillus subtilis Bacterial Adhesins Bacterial Physiology Binding Cell Survival Cell Wall Cell membrane Cell surface Cells Cellular Membrane Clostridium difficile Cytoplasm Data Defect Enzymes Foundations Genetic Transcription Goals Gram-Positive Bacteria Growth Healthcare Systems Homologous Gene Human In Vitro Infection Investigation Knowledge Laboratories Lipoproteins Membrane Proteins Messenger RNA Microbial Biofilms Modeling Molecular Organism Pathway interactions Physiological Play Polymers Process Proteins Research Personnel Role Staphylococcus aureus Streptococcus Streptococcus gordonii Streptococcus pyogenes Structure Surface Testing Translations Virulence Virulence Factors Weight Western Blotting cell type experimental study human disease interest lipoteichoic acid mutant oral commensal pathogenic bacteria protein expression scaffold virtual

项目摘要

Project Summary/Abstract Lipoteichoic acid (LTA) is an important polymer localized on the surface of Gram-positive bacteria. Discovered approximately 60 years ago, LTA comprises as much as 15% of the Gram- positive cell envelop dry weight, and LTA-defective strains are often non-viable or display severe growth defects, suggesting that LTA plays an important role in bacterial physiology. In spite of LTA's importance in cell viability and how long we have known of its existence, our understanding of LTA's role in bacterial physiology and how it contributes to survival remains obscure. The overarching goal of this project is to fill this knowledge gap. Using the oral commensal Streptococcus gordonii as a model Gram-positive organism, we show that LTA is required for the localization of the adhesin-like lipoprotein ScaA on the cell wall. No effect in transcription or steady-state level of ScaA protein was detected in other cellular compartment, which led us to hypothesize that LTA serves a structural role in ScaA localization on the cell wall. To test this hypothesis, we will (i) investigate the mechanisms involved in the cell “decision” to place ScaA on the cell wall, since lipoproteins like ScaA are expected to be retained within the cell membrane. (ii) We will determine whether ScaA directly binds LTA on the cell wall. And (iii) we will determine if LTA is involved in the cell wall localization of other proteins. These experiments will greatly enhance our understanding of this important Gram- positive structure and the underlying molecular mechanisms by which it affects surface-protein display, biofilm formation and, in some cases, cell viability.

项目总结/文摘