Mechanism of Viral Genome Delivery into Cells

病毒基因组传递至细胞的机制

基本信息

  • 批准号:
    10474828
  • 负责人:
  • 金额:
    $ 2.84万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-06-01 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

Project Summary from parent grant R35 GM140733 Nearly 120 years since the discovery of the first virus, our understanding of how viruses deliver genomes into cells overcoming the complexity of biological membranes remains limited. While a vast scientific literature exists on viral surface proteins and their interaction with host receptors, and the immune system, little emphasis has been devoted to studying the delivery of entire viral genomes into cells. For instance, how do bacteriophages eject DNA through the cell envelope of Gram-negative bacteria? Or, in humans, how do herpesviruses deliver ~200 kb genome through the Nuclear Pore Complex (NPC) into the cell nucleus? For a quarter of a century, first as a trainee (1995-2003), and since 2004 as a principal investigator, I have investigated the mechanisms of nucleocytoplasmic transport and viral genome packaging. My work has resulted in close to 85 publications that contributed to elucidating the atomic structure and regulation of crucial factors implicated in nuclear import, and viral genome packaging. In this R35, I propose to combine the study of these two seemingly distinct biological processes by focusing on the mechanisms of viral genome delivery into living cells. Specifically, I will ask two biological questions that seek to compare and contrast how simple bacterial viruses (or bacteriophages) eject their DNA into bacteria with how Herpesviruses deliver their complex genomes into the nucleus of eukaryotic cells. The first question explores how bacteriophages eject ~45 kb genomes through the cell envelope of gram-negative bacteria. Long-thought to be a simple pressure-driven injection, this process uses a virus-encoded nanomachine, which we have begun to study in my laboratory. The second question explores how Herpesviruses deliver their large genome through the Nuclear Pore Complex (NPC) of human cells into the cell nucleus. This is a signal- and energy-mediated process that uses host importins and the GTPase Ran, exploiting the cellular transport machinery to promote entry of an exogenous genome into the nucleus. Overall, understanding how viruses transfer genetic information through biological membranes into cells and organelles is vital for deciphering the molecular mechanisms of virulence as well as the development of novel therapeutic approaches. The common denominator of this R35 lies in our interest in the structure and transport mechanisms of biological macromolecules. Our research approach marries established sciences like protein biochemistry and X-ray crystallography with the power of cryo-electron microscopy (cryo-EM) to visualize biological macromolecules in near-native conditions. We believe that this R35 MIRA funding mechanism will fuel the creative and diligent pursuit of answers to the questions we pose, permitting our research program to achieve significant advancements in structural biology. Project Summary/Abstract Page 6
项目摘要来自家长批文R35 GM140733

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Gino Cingolani其他文献

Gino Cingolani的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Gino Cingolani', 18)}}的其他基金

Protein therapeutics inspired by importins
受进口蛋白启发的蛋白质疗法
  • 批准号:
    10506677
  • 财政年份:
    2022
  • 资助金额:
    $ 2.84万
  • 项目类别:
Mechanism of Viral Genome Delivery into Cells
病毒基因组传递至细胞的机制
  • 批准号:
    10727074
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
Mechanism of Viral Genome Delivery into Cells
病毒基因组传递至细胞的机制
  • 批准号:
    10413047
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
A humidity-controlled system for reprodicable vitrification of macromolecules
用于大分子可重复玻璃化的湿度控制系统
  • 批准号:
    10581231
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
Mechanism of Viral Genome Delivery into Cells
病毒基因组传递至细胞的机制
  • 批准号:
    10633050
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
Mechanism of Viral Genome Delivery into Cells
病毒基因组传递至细胞的机制
  • 批准号:
    10160588
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
Mechanism of Viral Genome Delivery into Cells
病毒基因组传递至细胞的机制
  • 批准号:
    10668228
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
Regulation of Nuclear Import Through Importin Alpha Isoforms
通过导入α亚型来调节核进口
  • 批准号:
    10083745
  • 财政年份:
    2018
  • 资助金额:
    $ 2.84万
  • 项目类别:
A NEW HYBRID DIFFRACTOMETER FOR MACROMOLECULAR CRYSTALLOGRAPHY AND SAXS AT TJU
天津大学用于高分子晶体学和 SAXS 的新型混合衍射仪
  • 批准号:
    8639253
  • 财政年份:
    2014
  • 资助金额:
    $ 2.84万
  • 项目类别:
Multisubunit viral ATPases that couple ATP-hydrolysis to genome translocation
将 ATP 水解与基因组易位耦合的多亚基病毒 ATP 酶
  • 批准号:
    9754155
  • 财政年份:
    2012
  • 资助金额:
    $ 2.84万
  • 项目类别:

相似海外基金

Engineered bacteriophages as biosensors for the rapid diagnosis of bacterial infection
工程噬菌体作为生物传感器,用于快速诊断细菌感染
  • 批准号:
    2879026
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Studentship
Designable, Orientable, and Responsive Photonic Crystals Based on Bacteriophages
基于噬菌体的可设计、可定向、响应灵敏的光子晶体
  • 批准号:
    23KJ0533
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
From cells to communities: The multi-scale impacts of bacteriophages in the gut microbiome
从细胞到群落:噬菌体对肠道微生物组的多尺度影响
  • 批准号:
    10714109
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
Bacteriophages in the ageing gut as targeted therapeutics
老化肠道中的噬菌体作为靶向治疗
  • 批准号:
    2893971
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Studentship
AI mining of the bacteriophages metagenome
噬菌体宏基因组的人工智能挖掘
  • 批准号:
    2890966
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Studentship
Demystifying virus-host interactions in Clostridioides difficile through genetic engineering of bacteriophages and the bacterial S-layer
通过噬菌体和细菌 S 层的基因工程揭开艰难梭菌中病毒与宿主相互作用的神秘面纱
  • 批准号:
    494839
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Operating Grants
Understanding cell surface recognition by bacteriophages to engineer novel therapeutics targeting Enterococcus cecorum, an emerging poultry pathogen
了解噬菌体的细胞表面识别,以设计针对盲肠肠球菌(一种新兴的家禽病原体)的新型疗法
  • 批准号:
    2881108
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Studentship
Bacteriophages against surgical site infections
噬菌体对抗手术部位感染
  • 批准号:
    10070793
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Collaborative R&D
Involvement of Bacteriophages in the Formation of Oral Bacterial Flora by Phageome Analysis
通过噬菌体分析了解噬菌体参与口腔细菌菌群的形成
  • 批准号:
    23H03074
  • 财政年份:
    2023
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Purification of bacteriophages using cascade-driven electrokinetic separation
使用级联驱动电动分离纯化噬菌体
  • 批准号:
    2133207
  • 财政年份:
    2022
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了