Core 1: Tumor Processing and Single Cell RNA sequencing Core

摘要 单细胞rna测序(scrna-seq)已经成为一种有效的方法来解剖肿瘤和 通过确定单个细胞的状态和推断部分遗传信息来确定它们的生态系统 从表达的文字记录中。对于渗入的T细胞，这提供了一种全面的方法来定义T 人类肿瘤中的细胞状态。对于恶性细胞，scRNA-seq为鉴定细胞特性铺平了道路 国家及其相关的基因类型在细胞分辨率。分子免疫学核心(核心1) 提供标准化和可重复性的方法来分析和表征免疫细胞和 在胶质母细胞瘤患者和小鼠模型中通过scrna-seq检测恶性细胞。核心1也 提供专家人员，促进计划内的知识共享，并维护 为所有项目生成数据所需的复杂设备。分子免疫学核心 将由Mario SUV ka博士和Kai Wucherpfennig博士领导，他们将贡献他们在单细胞领域的专业知识 技术和免疫细胞分离。

Abstract Single cell RNA-sequencing (scRNA-seq) has emerged as a powerful approach to dissect tumors and their ecosystems by determining the state of individual cells and inferring partial genetic information from expressed transcripts. For infiltrating T cells, this provides a comprehensive approach to define T cell states in human tumors. For malignant cells, scRNA-seq paves the way to characterizing cellular states and their associated genotypes at cellular resolution. The Molecular Immunology Core (Core 1) provides standardized and reproducible methods to profile and characterize immune cells and malignant cells by scRNA-seq in glioblastoma in both patients and mouse models. Core 1 also provides expert personnel, facilitates the sharing of knowledge within the program, and maintain the sophisticated equipment necessary to generate data for all Projects. The Molecular Immunology Core will be led Dr. Mario Suvà and Dr. Kai Wucherpfennig who will contribute their expertise in single-cell technologies and immune cell isolation.