Core 1: Tumor Processing and Single Cell RNA sequencing Core

核心1：肿瘤处理和单细胞RNA测序核心

• 批准号: 10684050
• 负责人: Kai W Wucherpfennig
• 金额: $ 34.98万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-03 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10684050
• 关键词: Animal Model; Blood; Brain Neoplasms; Cell Separation; Cells; Clinical; Clinical Trials; Clonal Expansion; Combination immunotherapy; Complement; Complex; DNA Sequencing Facility; Data; Data Set; Diagnosis; Ecosystem; Ensure; Epigenetic Process; Equipment; Experimental Models; Flow Cytometry; Gene Expression; Genetic; Genetic Transcription; Genomics; Genotype; Glioblastoma; Goals; Heterogeneity; Human; Human Resources; Immune; Immunologics; Immunosuppression; Immunotherapy; Individual; Knowledge; Length; Malignant Neoplasms; Methods; Molecular Immunology; Myeloid Cells; Operative Surgical Procedures; Pathway interactions; Patients; Peptide Vaccines; Phase; Population; Premalignant Cell; Quality Control; Reagent; Recurrence; Recurrent tumor; Regulatory Pathway; Relapse; Reproducibility; Research Personnel; Resolution; Resources; Sampling; Services; Specimen; Standardization; T cell infiltration; T-Lymphocyte; Techniques; Technology; Testing; Transcript; anti-PD1 antibodies; antigen-specific T cells; cancer cell; cancer infiltrating T cells; conventional therapy; data sharing; genetic information; mouse model; neoantigen vaccine; neoantigens; novel therapeutic intervention; participant enrollment; programs; single cell technology; single-cell RNA sequencing; success; technology platform; tool; transcriptome; tumor

Abstract Single cell RNA-sequencing (scRNA-seq) has emerged as a powerful approach to dissect tumors and their ecosystems by determining the state of individual cells and inferring partial genetic information from expressed transcripts. For infiltrating T cells, this provides a comprehensive approach to define T cell states in human tumors. For malignant cells, scRNA-seq paves the way to characterizing cellular states and their associated genotypes at cellular resolution. The Molecular Immunology Core (Core 1) provides standardized and reproducible methods to profile and characterize immune cells and malignant cells by scRNA-seq in glioblastoma in both patients and mouse models. Core 1 also provides expert personnel, facilitates the sharing of knowledge within the program, and maintain the sophisticated equipment necessary to generate data for all Projects. The Molecular Immunology Core will be led Dr. Mario Suvà and Dr. Kai Wucherpfennig who will contribute their expertise in single-cell technologies and immune cell isolation.

抽象的 单细胞RNA - 序列（SCRNA-SEQ）已成为一种剖析肿瘤和的强大方法 通过确定单个细胞的状态并推断部分遗传信息，它们的生态系统 来自表达的成绩单。对于浸润的T细胞，这提供了定义T的全面方法 人类肿瘤中的细胞状态。对于恶性细胞，SCRNA-Seq为表征细胞的方式铺平了道路 在细胞分辨率下的状态及其相关的基因型。分子免疫学核心（核心1） 提供标准化且可重复的方法来谱图和表征免疫细胞，以及 患者和小鼠模型中的胶质母细胞瘤中通过SCRNA-SEQ进行恶性细胞。核心1也是 提供专家人员，促进计划中的知识共享，并维护 为所有项目生成数据所需的软化设备。分子免疫学核心 将领导MarioSuvà博士和Kai Wucherpfennig博士，他们将在单细胞中贡献他们的专业知识 技术和免疫细胞隔离。