NMDOH Scientific Laboratory Division Retail Meat Surveillance Program

项目摘要 目的 新墨西哥州卫生部科学实验室司(NMDOH SLD) 自2004年以来一直参与零售肉类监测(RMS)。RMS研究 传统上专注于零售肉类样品，如生鸡，碎火鸡，碎牛肉， 以及来自农场活体动物的猪肉，如牛、家禽、 还有猪。这些肉类基质中的相关细菌靶标是 沙门氏菌属、弯曲杆菌属(主要是空肠弯曲杆菌和大肠埃希菌)、 肠球菌和大肠埃希菌。近期国家抗菌素耐药性报告 监控系统(NARMS)计划已向战略方向迈进， 强调一个健康监测模式，这是一个多部门和 跨学科的方法，着眼于人、动物、 植物和环境。这已经并将继续带来新的先导研究。 这将把检测扩大到其他肉类基质以及环境样本，如 作为地表水和土壤。 NMDOH SLD的环境微生物学(EM)实验室目前 参与了NARMS最新的关于海鲜的试点研究，并在 分离预定目标：弧菌、气单胞菌、肠球菌、 和乳糖发酵剂。EM实验室乐于参与先导研究和 目前已对一些方法进行了修改，以更好地提高部分上市产品的回收率 目标。海鲜试点研究特别重要，因为海鲜的消费 海产品，特别是水产养殖产品，预计将大幅增加， 工业严重依赖抗生素的使用来对抗威胁人类健康的传染病 制作。 购买活动一直受到限制，就每月购物频率而言 生肉样品和此类操作的时间分配(不超过3小时)，原因是 缺乏配备P卡的合格采购人员。EM实验室 最近提供了一张P卡，这将使扩大购物成为可能 为即将到来的赠款周期开始提供样本收集能力。将不再是 EM实验室每月只能举办一次购物活动，但正在计划 每周去一次。EM实验室人员将另外能够购买 来自整个新墨西哥州其他城市的零售肉类样本 是不可能的。这种时间和地理上的扩展总体上应该提供 对NARMS计划更有意义的数据。 预期结果 这项研究的目的是确定人群中抗菌素耐药性的流行率。 零售样品中沙门氏菌、弯曲杆菌、大肠杆菌和肠球菌的分离 年从杂货店购买的鸡肉、火鸡末、牛肉末和猪排 新墨西哥州。所获得的信息也将帮助我们的流行病学家更好地 了解新墨西哥州抗菌素耐药性的流行情况。实验室将会 继续按照既定程序为该项目提供测试，并 参与任何引入的试点研究。为EM批准P-卡 部分将允许在五年内向新墨西哥州提供额外的保险 格兰特项目。 第1页，共1页

Project Summary Purpose The New Mexico Department of Health Scientific Laboratory Division (NMDOH SLD) has participated in the Retail Meat Surveillance (RMS) since 2004.The RMS study has traditionally focused on retail meat samples like raw chicken, ground turkey, ground beef, and pork that are derived from farm based live-stock animals such as bovines, poultry, and swine. The associated bacterial targets from these meat matrices have been Salmonella species, Campylobacter species (primarily C. jejuni and C. coli), Enterococcus species, and E. coli. Recently the National Antimicrobial Resistance Monitoring System (NARMS) program has moved in a strategic direction with an emphasis on a One Health model of monitoring, which is a multisectoral and transdisciplinary approach that looks at the interconnections between people, animals, plants, and the environment. This has and will continue to bring about new pilot studies that will expand testing to other meat matrices as well as to environmental samples such as surface waters and soils. The Environmental Microbiology (EM) Laboratory at NMDOH SLD is currently participating in the latest NARMS pilot study involving seafood and is doing well with isolating the intended targets: Vibrio species, Aeromonas species, Enterococcus species, and Lactose fermenters. EM Laboratory enjoys participating in the pilot study and currently has some method modifications to better improve recovery of some of the listed targets. The seafood pilot study is particularly important because the consumption of seafood, particularly aquaculture products, is projected to significantly increase and such industries rely heavily on the use of antibiotics to combat infectious diseases that threaten production. Purchasing activities have been limited, in terms of the monthly shopping frequency for raw meat samples and time allotments for such operations (limited to 3 hours), due to a shortage of qualified purchasing personnel equipped with P-cards. The EM Laboratory was recently provided with a P-card which will make it possible to expand shopping / sample gathering capabilities for the start of this upcoming grant cycle. No longer will the EM Laboratory be limited to just one shopping event per month, but instead is planning on going once per week. EM Laboratory personnel will additionally be able to purchase retail meat samples from other cities across the entire state of New Mexico that previously was not possible. Such temporal and geographical expansions should overall provide for more meaningful data to the NARMS program. Expected Outcomes The goal of this study is to determine the prevalence of antimicrobial resistance among Salmonella, Campylobacter, E. coli and Enterococci isolated from retail samples of retail chicken, ground turkey, ground beef and pork chops purchased from grocery stores in New Mexico. The information obtained will also help our epidemiologists better understand the prevalence of antimicrobial resistance in New Mexico. The laboratory will continue to provide testing for this project following established procedures and also participate in any pilot studies that are introduced. The approval of a P-card for the EM section will allow for additional coverage with the state of New Mexico over the five-year grant project. Page 1 of 1