Neuropathology and Biochemistry Core

项目摘要-神经病理学和生物化学核心 神经病理评估仍然是痴呆临床和翻译研究的重要组成部分 路易小体(DLB)和帕金森病痴呆症(PDD)，已被归类为 美国国立卫生研究院赞助的阿尔茨海默病和相关痴呆(ADRD)的研究建议 推荐为路易体痴呆(LBD)。核心B：神经病理学的目的是 对所有LBD大脑进行量化的死后神经病理评估和数据收集， 分发给CWOW的科学家。核心B将提供粉碎的冷冻人脑样本，大脑 腰椎间盘突出症患者和配对病例的匀浆、纯化蛋白制剂(Aβ和α-Syn)或石蜡切片 对所有CWW调查人员以及其他研究机构的合格调查人员进行控制 由核心A执行委员会和梅奥诊所生物医学委员会批准。这个 产生的数量性状包括皮质路易小体的计数，对实质的半定量估计 黑质神经元丢失、壳核多巴胺能缺陷的酪氨酸羟化酶免疫组织化学和免疫组织化学 S硫代黄素对皮质及皮质下区老年斑和神经原纤维缠结的计数 荧光显微镜。岩芯B将对处理过的切片进行数字显微镜和图像分析 α-Syn、磷酸化tau和Aβ40、Aβ42的免疫组织化学染色 内表型，如磷酸化TDP-43。

PROJECT SUMMARY – NEUROPATHOLOGY AND BIOCHEMISTRY CORE Neuropathologic evaluation remains an important part of clinical and translational research on dementia with Lewy bodies (DLB) and Parkinsonian disease dementia (PDD), disorders that have been grouped in the research recommendations of NIH-sponsored Alzheimer Disease and Related Dementias (ADRD) recommendations as the Lewy body dementias (LBD). The purpose of the Core B: Neuropathology is to perform quantitative postmortem neuropathologic evaluations and data collection for all LBD brains that will be distributed to the CWOW scientists. The Core B will provide pulverized frozen human brain samples, brain homogenates, purified protein preparations (Aβ and α-Syn) or paraffin sections of LBD cases and matched controls to all CWOW investigators, as well as to qualified investigators at other research institutions as approved by the Executive Committee of Core A and the Mayo Clinic Biospecimen Committee. The quantitative traits generated include counts of cortical Lewy bodies, semiquantitative estimates of substantia nigra neuronal loss, dopaminergic deficiency in putamen with tyrosine hydroxylase immunohistochemistry and counts of senile plaques and neurofibrillary tangles in cortical and subcortical areas with thioflavin S fluorescent microscopy. Core B will perform digital microscopy and image analysis on sections processed for immunohistochemistry for α-Syn, phospho-tau and Aβ40, Aβ42, as additional other quantitative endophenotypes, such as phospho-TDP-43.