Neuropathology Core

项目描述/摘要-神经病理学核心 神经病理学（NP）核心进行诊断评估，对脑组织进行定量分析 在马约阿尔茨海默病研究中心（ADRC）对参与者进行尸检时收集， 测试已知和新的遗传变异。NP核心为ADRC附属调查人员提供支持 和外部调查人员。NP Core生成的神经病理学和遗传学数据是 传达给数据管理和统计核心，谁上传到国家阿尔茨海默病 协调中心。NP Core的研究方法如下：具体目标1。进行脑部 对马约ADRC的参与者进行尸检，并及时按照协议进行，其中包括 收集固定和冷冻的组织。具体目标2。提供神经病理学评估并收集 使用大体解剖和神经组织学的标准化方法获得神经病理学数据。分配AD 基于塔尔淀粉样蛋白阶段、Braak NFT分期和CERAD神经炎斑块评分的可能性诊断 使用NIA-AA诊断概率方案。就临床病理诊断达成共识， 视频会议。在NACC版本10上记录神经病理学数据，并提供给数据管理部门 统计核心和NACC。具体目标3。使用数字生成定量内表型 病理学和图像分析支持ADRC研究。具体目标4。储存脑组织和其他 尸检衍生材料，并提供临床和病理学表征良好的组织样本， 研究人员在马约诊所和外部机构。通过连续使用确保生物样本的安全性 电子监控冰柜，并始终保持至少一个空的备用冰柜。 具体目标5.向ADRC提供遗传学专业知识，以及筛查已知和新发现的 AD、路易体疾病（LBD）和额颞叶疾病的基因或常见遗传风险变体 退化（FTLD）。

PROJECT DESCRIPTION/ABSTRACT – NEUROPATHOLOGY CORE The Neuropathology (NP) Core performs diagnostic evaluations, quantitative analyses on brain tissue collected at autopsy of participants in the Mayo Alzheimer Disease Research Center (ADRC), and genetic testing of known and novel genetic variants. The NP Core provides support to ADRC-affiliated investigators and to outside investigators. The neuropathologic and genetic data generated by the NP Core is communicated to the Data Management and Statistical Core, who uploads it to the National Alzheimer Coordinating Center. The research approach for the NP Core is as follows: Specific Aim 1. Perform brain autopsies on participants of the Mayo ADRC in a timely fashion and according to protocol, which includes collection of fixed and frozen tissue. Specific Aim 2. Provide neuropathologic evaluations and collect neuropathologic data using standardized methods for gross dissection and neurohistology. Assign AD likelihood diagnoses based upon Thal amyloid phase, Braak NFT stage, and CERAD neuritic plaque score using the NIA-AA diagnostic probability scheme. Arrive at a consensus on clinicopathologic diagnoses at videoconferences. Record neuropathologic data on NACC Version 10 and provide to the Data Management and Statistical Core and NACC. Specific Aim 3. Generate quantitative endophenotypes using digital pathology and image analysis in support of ADRC research. Specific Aim 4. Store brain tissue and other autopsy-derived materials and provide clinically and pathologically well-characterized tissue samples to investigators at Mayo Clinic and outside institutions. Assure security of biologic samples by using continuous electronic monitoring of freezers and by maintaining at all times at least one empty back-up freezer. Specific Aim 5. Provide genetic expertise to ADRC, as well as screening for known and newly discovered genes or common genetic risk variants for AD, Lewy body disorders (LBD) and frontotemporal lobar degenerations (FTLD).