Neuropathology Core

项目说明/摘要-神经病理学核心 神经病理学(NP)核心对脑组织进行诊断评估、定量分析 在梅奥阿尔茨海默病研究中心(ADRC)参与者的尸检中收集，以及遗传学 检测已知的和新的基因变异。NP核心为与ADRC有关的调查人员提供支持 以及外部调查人员。由NP Core生成的神经病理和遗传数据是 传达给数据管理和统计核心，后者将其上传到国家阿尔茨海默病 协调中心。NP核心的研究方法如下：具体目标1.执行大脑 及时并根据议定书对梅奥民主与和解委员会参与者进行尸检，其中包括 收集固定的和冷冻的组织。具体目标2.提供神经病理评估并收集 使用大体解剖和神经组织学的标准化方法获得神经病理学数据。分配AD 基于Thal淀粉样时相、Braak NFT分期和CERAD神经性斑块评分的可能性诊断 使用NIA-AA诊断概率方案。就临床病理诊断达成共识 视频会议。记录NACC版本10上的神经病理数据并提供给数据管理 和统计核心和NACC。具体目标3.使用数字技术生成定量的内表型 病理和图像分析，以支持ADRC研究。具体目标4.储存脑组织和其他 身体解剖衍生材料，并提供临床和病理特征良好的组织样本 梅奥诊所和外部机构的调查人员。用连续的方法确保生物样本的安全 对冰柜进行电子监测，并始终保持至少一个空的备用冰柜。 具体目标5.向红十字和红新月会提供遗传专业知识，并对已知和新发现的 AD、路易体病(LBD)和额颞叶的基因或常见遗传风险变异 退行性疾病(FTLD)。

PROJECT DESCRIPTION/ABSTRACT – NEUROPATHOLOGY CORE The Neuropathology (NP) Core performs diagnostic evaluations, quantitative analyses on brain tissue collected at autopsy of participants in the Mayo Alzheimer Disease Research Center (ADRC), and genetic testing of known and novel genetic variants. The NP Core provides support to ADRC-affiliated investigators and to outside investigators. The neuropathologic and genetic data generated by the NP Core is communicated to the Data Management and Statistical Core, who uploads it to the National Alzheimer Coordinating Center. The research approach for the NP Core is as follows: Specific Aim 1. Perform brain autopsies on participants of the Mayo ADRC in a timely fashion and according to protocol, which includes collection of fixed and frozen tissue. Specific Aim 2. Provide neuropathologic evaluations and collect neuropathologic data using standardized methods for gross dissection and neurohistology. Assign AD likelihood diagnoses based upon Thal amyloid phase, Braak NFT stage, and CERAD neuritic plaque score using the NIA-AA diagnostic probability scheme. Arrive at a consensus on clinicopathologic diagnoses at videoconferences. Record neuropathologic data on NACC Version 10 and provide to the Data Management and Statistical Core and NACC. Specific Aim 3. Generate quantitative endophenotypes using digital pathology and image analysis in support of ADRC research. Specific Aim 4. Store brain tissue and other autopsy-derived materials and provide clinically and pathologically well-characterized tissue samples to investigators at Mayo Clinic and outside institutions. Assure security of biologic samples by using continuous electronic monitoring of freezers and by maintaining at all times at least one empty back-up freezer. Specific Aim 5. Provide genetic expertise to ADRC, as well as screening for known and newly discovered genes or common genetic risk variants for AD, Lewy body disorders (LBD) and frontotemporal lobar degenerations (FTLD).