Live Cell Fluorescent Assays for SARS-CoV-2 protease activity and COVID-19 Drug Discovery

摘要报表 SARS-CoV-2病毒依赖于两种酶的复制：3CLPro和 PLPRO.如果这两种酶中的一种或两种都能被阻断，新冠肺炎感染就可以 有效治疗。蒙大拿州分子制造和销售活细胞，荧光 用于疾病研究和药物发现的生物传感器分析。为回应这一事件 新冠肺炎大流行，蒙大拿分子将其专业知识和背景应用于 建立SARS-CoV-2荧光生物传感器的活细胞检测技术 主要蛋白水解酶3CLPro。本建议书中描述的原型传感器 论证了可行性。此处请求直接至第二阶段支持，以加快 开发更明亮、更灵敏的3CLPRO和PLPRO检测方法 蛋白酶。这些检测方法可用于化合物的高通量筛选。 抑制蛋白水解酶，并与活着的人类细胞中的目标进行接触。 马拉丁公园大道套房A Bozeman，MT 59715

SUMMARY STATEMENT The SARS-CoV-2 virus depends upon two proteases for replication, 3CLpro and PLpro. If one or both of these proteases can be blocked, COVID-19 infection can be effectively treated. Montana Molecular makes and sells live cell, fluorescent biosensor assays for disease research and drug discovery. In response to the COVID-19 pandemic, Montana Molecular applied its expertise and background in live cell assay development to build fluorescent biosensors for the SARS-CoV-2 main protease, 3CLpro. The prototype sensor described in this proposal demonstrates feasibility. Direct to Phase II support is requested here to speed the development of even brighter, more sensitive assays for the 3CLpro and PLpro proteases. These assays can be used in high throughput screening for compounds that inhibit the protease, and engage with their target in living, human cells. 406-539-7399 366 Gallatin Park Drive Suite A Bozeman, MT 59715