Live Cell Fluorescent Assays for SARS-CoV-2 protease activity and COVID-19 Drug Discovery

SARS-CoV-2 蛋白酶活性和 COVID-19 药物发现的活细胞荧光测定

• 批准号: 10621803
• 负责人: THOMAS E HUGHES
• 金额: $ 30万
• 依托单位: MONTANA MOLECULAR, LLC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-13 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10621803
• 关键词: 2019-nCoV; Biological; Biological Assay; Biosensor; COVID-19; COVID-19 assay; COVID-19 pandemic; COVID-19 therapeutics; COVID-19 treatment; Cells; Cellular Assay; Coronavirus; Development; Dimerization; Disease; Drug Screening; Enzymes; Fluorescence; Goals; Health; Hour; Human; Image; Immunohistochemistry; Incubated; Intercalated Cell; Libraries; Liquid substance; Measurable; Measurement; Methods; Microscopy; Molecular; Montana; Noise; Nonstructural Protein; Peptide Hydrolases; Personal Satisfaction; Pharmaceutical Preparations; Phase; Protease Inhibitor; Proteins; Reader; Reporting; Research; SARS-CoV-2 genome; SARS-CoV-2 infection; SARS-CoV-2 protease; Signal Transduction; Site; Speed; Testing; Time; Translating; Viral; Virus; Virus Replication; assay development; cell type; combat; design; drug discovery; high throughput screening; inhibitor; lead optimization; prototype; response; screening; sensor; targeted treatment; tool; vector

SUMMARY STATEMENT The SARS-CoV-2 virus depends upon two proteases for replication, 3CLpro and PLpro. If one or both of these proteases can be blocked, COVID-19 infection can be effectively treated. Montana Molecular makes and sells live cell, fluorescent biosensor assays for disease research and drug discovery. In response to the COVID-19 pandemic, Montana Molecular applied its expertise and background in live cell assay development to build fluorescent biosensors for the SARS-CoV-2 main protease, 3CLpro. The prototype sensor described in this proposal demonstrates feasibility. Direct to Phase II support is requested here to speed the development of even brighter, more sensitive assays for the 3CLpro and PLpro proteases. These assays can be used in high throughput screening for compounds that inhibit the protease, and engage with their target in living, human cells. 406-539-7399 366 Gallatin Park Drive Suite A Bozeman, MT 59715

简要说明 SARS-CoV-2病毒的复制依赖于两种蛋白酶，3CLpro和 PLpro。如果这些蛋白酶中的一种或两种都能被阻断，COVID-19感染就可以被阻断。 有效治疗。蒙大拿分子制造和销售活细胞，荧光 用于疾病研究和药物发现的生物传感器测定。响应于 2019冠状病毒病大流行，蒙大拿分子应用其专业知识和背景， 建立SARS-CoV-2荧光生物传感器的活细胞检测开发 主蛋白酶3CLpro。本提案中描述的原型传感器 证明了可行性。直接到第二阶段的支持是在这里要求，以加快 开发更明亮、更灵敏的3CLpro和PLpro检测试剂盒 蛋白酶这些分析可用于高通量筛选化合物 抑制蛋白酶，并与活的人类细胞中的目标结合。 406-539-7399 366 Gallatin Park Drive Suite A Bozeman，MT 59715