Broad-based spike protein stalk-based vaccine platform for SARS-CoV-2 and other coronaviruses

项目总结/摘要 我们建议使用基于结构的方法开发针对SARS-CoV-2的亚单位疫苗， 病毒刺突蛋白茎区的保守和功能必需结构域。我们的疫苗 该平台适用于各种现有和新出现的冠状病毒并有效。作为我们 系统是基于E.大肠杆菌，它预计将是成本效益，我们的茎为基础的方法， 专门设计用于涵盖一系列不同的冠状病毒。然而，值得注意的是，我们的疫苗 该平台具有高度灵活性，面对新型冠状病毒， 可能会出现这种情况，而本申请中开发的疫苗对此无效。

Project Summary/Abstract We propose to develop a subunit vaccine for SARS-CoV-2, using a structure-based approach targeting conserved and functionally essential domains in the stalk region of the viral spike protein. We expect our vaccine platform to be applicable and effective across a wide range of existing and emerging coronaviruses. As our system is based on expression in E. coli is it expected to be cost–effective, and our stalk-based approach is specifically designed to cover a range of distinct coronaviruses. However, it is important to note that our vaccine platform is highly flexible, with the antigen able to be re-engineered rapidly in the face of a novel coronavirus that may emerge, and for which the vaccine developed in this application is not effective.