Broad-based spike protein stalk-based vaccine platform for SARS-CoV-2 and other coronaviruses

项目摘要/摘要 我们建议开发一种SARS-CoV-2亚单位疫苗，采用基于结构的靶向方法 病毒刺突蛋白茎部区域的保守和功能必需结构域。我们期待我们的疫苗 该平台可适用于各种现有和新出现的冠状病毒，并能有效应对。作为我们的 系统是基于在大肠杆菌中表达的，它预计是经济有效的，而我们基于茎的方法是 专门设计来覆盖一系列不同的冠状病毒。然而，重要的是要注意到我们的疫苗 平台高度灵活，面对一种新的冠状病毒，抗原能够迅速重新设计 可能会出现，而在本申请中开发的疫苗对此无效。

Project Summary/Abstract We propose to develop a subunit vaccine for SARS-CoV-2, using a structure-based approach targeting conserved and functionally essential domains in the stalk region of the viral spike protein. We expect our vaccine platform to be applicable and effective across a wide range of existing and emerging coronaviruses. As our system is based on expression in E. coli is it expected to be cost–effective, and our stalk-based approach is specifically designed to cover a range of distinct coronaviruses. However, it is important to note that our vaccine platform is highly flexible, with the antigen able to be re-engineered rapidly in the face of a novel coronavirus that may emerge, and for which the vaccine developed in this application is not effective.