Laser particles for multi-dimensional single-cell analysis

项目摘要 这项工作的主要目标是通过引入新的细胞条形码来推进单细胞分析 技术.了解细胞是生命科学的基石。单细胞测序导致了一个范例 我们分析细胞的方法从整体到单个细胞的转变。不幸的是，目前的单细胞 分析技术几乎完全是静态的，离体进行，因此不能容易地探测动态的 细胞过程除了静态分析之外，下一个重要的步骤是添加更多的维度： 使细胞成为一个有生命的实体，包括时间变化，空间运动，与体内其他细胞的相互作用。 该项目将开发激光粒子（LP），它可以在真实的时间内以光学方式读取，并可重复读取， 需要，用于多维单细胞分析。利用光学条形码，可以从 在不同的时间，地点和设备的细胞，并汇编数据，以产生细胞的完整帐户。一 LP与寡核苷酸条形码的组合实现了获取大规模数据的突破性方法 从体内生理学到串联使用多台仪器的分子组学。该项目组织在 三个具体目标。目的1开发与寡核苷酸条形码相结合的大规模LP条形码。Aim 2结构 几种仪器将LP条形码阅读器集成到商业平台中。目标3涉及验证 研究以证明新技术的即时效用。光学条形码预计将 在单细胞分析中做出了一个范例的改变。这将改变多维单细胞 分析用于医疗保健中的科学发现以及诊断和治疗应用。

Project Summary The broad objective of this proposed work is to advance single-cell analysis by introducing novel cell-barcoding technologies. Understanding cells is a cornerstone of life sciences. Single-cell sequencing led to a paradigm shift in our approach to analyze cells from ensembles to individual cells. Unfortunately, the current single-cell analysis techniques are almost exclusively static, performed ex vivo, and thus cannot easily probe the dynamic cellular processes. Beyond static analysis, the next important step is to add more dimensions: everything that makes a cell a living entity, including temporal changes, spatial movement, interactions with other cells in vivo. This project will develop laser particles (LPs), which can be read optically in real time and repeatedly as needed, for multi-dimensional single-cell analysis. With the optical barcode, it is possible to acquire data from a cell at different times, locations and apparatuses, and compile the data to produce a full account of the cell. A combination of LPs with oligonucleotide barcodes enables a breakthrough approach acquiring large-scale data from in vivo physiology to molecular omics using multiple instruments in series. This project is organized in three specific aims. Aim 1 develops large-scale LP barcodes combined with oligo barcodes. Aim 2 constructs several instruments integrating LP barcode readers into commercial platforms. Aim 3 involves validation studies to demonstrate the immediate utilities of the new technologies. The optical barcoding is expected to make a paradigm change in single-cell analysis. This will transform the way multi-dimensional single-cell analysis is used for scientific discovery and diagnostic and therapeutic applications in healthcare.