Matricellular protein CCN3 in vascular homeostasis
基质细胞蛋白 CCN3 在血管稳态中的作用
基本信息
- 批准号:10504077
- 负责人:
- 金额:$ 67.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-07-15 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAngiotensin IIAnimalsAnti-Inflammatory AgentsAortaAortic AneurysmAtlasesAutomobile DrivingBehaviorBiochemicalBlood VesselsCellsChestCommunicationDevelopmentDilatation - actionDissectionEndothelial CellsEndotheliumEnvironmental Risk FactorEventExhibitsExposure toExtracellular Matrix DegradationFoundationsFunctional disorderGene FamilyGleanHealthHistologicHomeostasisIn VitroIncidenceInfiltrationInflammasomeInflammationInfusion proceduresKnockout MiceKnowledgeLaboratoriesLeadLeftLeukocytesMaintenanceMarfan SyndromeMedicalModelingMolecularMorbidity - disease rateMusMutationOperative Surgical ProceduresOrganOxidative StressPathogenesisPathologicPharmacologyPhysiologicalPhysiologyPlant RootsPlayPreventionPropertyProteinsReactive Oxygen SpeciesRegulationResearchRoleRuptureSecondary toSeveritiesSignal PathwayTherapeuticThoracic Aortic AneurysmTissuesVascular DiseasesVascular EndotheliumWorkcell typeconditional knockoutendothelial dysfunctionexperimental studygene networkin vivoinhibitorinsightmembermortalitymouse modelnovelnovel therapeutic interventionnovel therapeuticspreventsingle-cell RNA sequencingtranscriptome sequencingvascular inflammation
项目摘要
SUMMARY
Aortic homeostasis is essential for vascular health. The vascular endothelium is a vital organ whose health is
essential to normal vascular physiology; when dysregulated, it becomes a critical factor in the pathogenesis
of a variety of vascular diseases. Growing evidence indicates that endothelial cells (ECs) play an
underappreciated role in the development and progression of thoracic aortic aneurysm and dissection (TAAD).
Most notably, compromised barrier integrity has been implicated as a precursor to the development of TAA.
Work from our laboratory has led to the appreciation that a member of Cellular Communication Network (CCN)
gene family, CCN3, serves as an important regulator of vascular health. However, the precise physiological
role of CCN3 in vivo as well as the fundamental mechanisms responsible for CCN3’s action remain largely
undefined. Recently, our preliminary studies suggest that CCN3 serves as an endogenous inhibitor of TAAD.
Recently, our preliminary studies suggest that endothelial dysfunction due to CCN3 deficiency predisposes
animals to thoracic aortic aneurysm and dissection (TAAD). In a mouse model of Marfan syndrome
(Fbn1mgR/mgR), global deficiency of CCN3 deficiency exacerbates thoracic aortic aneurysm (TAA) in Marfan
mice characterized by increased aortic root and ascending thoracic aortic dilation, elevated incidence of
vessel dissection, and, as a consequence, enhanced mortality. Similar findings were obtained when
challenging animals with angiotensin II (Ang II). Endothelial restricted knockout mice (EC CCN3-/-)
subjected to Ang II infusion exhibited augmented aortic root and thoracic aortic dilation along with an elevated
severity of aortic wall destruction. Mechanistically, our initial bulk RNAseq studies suggested that loss of
CCN3 perturbs vascular junction and causes aberrant activation of signaling pathways involved in
inflammation. Our subsequent in vivo and in vitro studies further support that key factors secondary to CCN3
deficiency (endothelial barrier disruption, inflammation, reactive oxygen species (ROS)) cause endothelial
dysfunction and play a pivotal role in TAAD development and progression. These observations provide the
foundation for the central hypotheses of this application: CCN3 serves as a critical physiological regulator for
the maintenance of endothelial function essential for the prevention of TAAD. Studies in this proposal will
rigorously address this hypothesis. The role of endothelial CCN3 in mitigating TAAD will be fully characterized.
Mechanistic basis of how CCN3 deficiency leads to endothelial dysfunction will be investigated. The results
of these studies will elucidate the role of CCN3 in controlling endothelial function and the mechanisms
underlying its ability to promote endothelial barrier integrity and limit inflammation.
总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Zhiyong Lin其他文献
Zhiyong Lin的其他文献
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{{ truncateString('Zhiyong Lin', 18)}}的其他基金
Matricellular protein CCN3 in vascular homeostasis
基质细胞蛋白 CCN3 在血管稳态中的作用
- 批准号:
10662518 - 财政年份:2022
- 资助金额:
$ 67.78万 - 项目类别:
Deciphering the regulatory role of matricelluar protein CCN3 in functional collateral blood flow
解读基质细胞蛋白CCN3在功能性侧支血流中的调节作用
- 批准号:
10594955 - 财政年份:2020
- 资助金额:
$ 67.78万 - 项目类别:
Deciphering the regulatory role of matricelluar protein CCN3 in functional collateral blood flow
解读基质细胞蛋白CCN3在功能性侧支血流中的调节作用
- 批准号:
10371083 - 财政年份:2020
- 资助金额:
$ 67.78万 - 项目类别:
Role of Protein Phosphatase 2A in Aortic Aneurysm
蛋白磷酸酶 2A 在主动脉瘤中的作用
- 批准号:
10317079 - 财政年份:2019
- 资助金额:
$ 67.78万 - 项目类别:
KLF15 and circadian regulation of alcohol-induced liver injury
KLF15 与酒精性肝损伤的昼夜节律调节
- 批准号:
9212759 - 财政年份:2014
- 资助金额:
$ 67.78万 - 项目类别:
KLF15 and circadian regulation of alcohol-induced liver injury
KLF15 与酒精性肝损伤的昼夜节律调节
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9000080 - 财政年份:2014
- 资助金额:
$ 67.78万 - 项目类别:
KLF15 and circadian regulation of alcohol-induced liver injury
KLF15 与酒精性肝损伤的昼夜节律调节
- 批准号:
8576602 - 财政年份:2014
- 资助金额:
$ 67.78万 - 项目类别:
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