NMR Core

核磁共振核心

• 批准号: 10506950
• 负责人: ANGELA M. GRONENBORN
• 金额: $ 96.87万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10506950
• 关键词: Capsid; Cell Nucleus; Cells; Communities; Complex; Data Analyses; Detection; Disease; Environment; Equipment; Fluorine; Frequencies; Future; Goals; HIV; HIV-1; Institution; Integrase; Integrase Inhibitors; Integration Host Factors; Magic; Measurement; Measures; Methodology; Methods; Molecular Conformation; NMR Spectroscopy; Nature; Nuclear; Nuclear Import; Physiological; Positioning Attribute; Proteins; Protocols documentation; Recombinant Proteins; Research; Research Personnel; Resolution; Resources; Savings; Site; Spin Labels; Structure; Technology; Time; Work; base; biophysical properties; cell type; data acquisition; design; experimental study; in vivo; inhibitor; innovation; insight; instrumentation; magnetic field; new technology; protein complex; protein structure; screening; solid state; solid state nuclear magnetic resonance; structural biology; trafficking

NMR Core The overall goal of the NMR Core is to provide state-of-the art capabilities for PCHPI researchers, for atomic- level characterization of structure and dynamics of HIV-1 proteins and their complexes. To accomplish this goal, we will take advantage of superb instrumentation resources, innovative experimental protocols, and unique expertise developed during the past decade. Specific aims are designed to i) support PCHPI projects and extra- center collaborators by providing cutting-edge solid-state NMR capabilities for HIV-1 research, and ii) develop in-cell solution and solid state 19F NMR for HIV-1 research. The novel technologies developed by the NMR Core will have major impact on HIV-1 cellular structural biology.

核磁共振岩心 NMR核心的总体目标是为PCHPI研究人员提供最先进的能力， HIV-1蛋白及其复合物的结构和动力学水平表征。为了实现这一目标， 我们将利用一流的仪器资源，创新的实验方案， 在过去十年中发展的专业知识。具体目标是：（一）支持PCHPI项目和额外的 通过为HIV-1研究提供尖端的固态NMR能力，为合作者提供中心，以及ii）开发 用于HIV-1研究的细胞内溶液和固态19 F NMR。NMR Core开发的新技术 将对HIV-1的细胞结构生物学产生重大影响。