NMR Core

核磁共振核心

• 批准号: 10506950
• 负责人: ANGELA M. GRONENBORN
• 金额: $ 96.87万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10506950
• 关键词: Capsid; Cell Nucleus; Cells; Communities; Complex; Data Analyses; Detection; Disease; Environment; Equipment; Fluorine; Frequencies; Future; Goals; HIV; HIV-1; Institution; Integrase; Integrase Inhibitors; Integration Host Factors; Magic; Measurement; Measures; Methodology; Methods; Molecular Conformation; NMR Spectroscopy; Nature; Nuclear; Nuclear Import; Physiological; Positioning Attribute; Proteins; Protocols documentation; Recombinant Proteins; Research; Research Personnel; Resolution; Resources; Savings; Site; Spin Labels; Structure; Technology; Time; Work; base; biophysical properties; cell type; data acquisition; design; experimental study; in vivo; inhibitor; innovation; insight; instrumentation; magnetic field; new technology; protein complex; protein structure; screening; solid state; solid state nuclear magnetic resonance; structural biology; trafficking

NMR Core The overall goal of the NMR Core is to provide state-of-the art capabilities for PCHPI researchers, for atomic- level characterization of structure and dynamics of HIV-1 proteins and their complexes. To accomplish this goal, we will take advantage of superb instrumentation resources, innovative experimental protocols, and unique expertise developed during the past decade. Specific aims are designed to i) support PCHPI projects and extra- center collaborators by providing cutting-edge solid-state NMR capabilities for HIV-1 research, and ii) develop in-cell solution and solid state 19F NMR for HIV-1 research. The novel technologies developed by the NMR Core will have major impact on HIV-1 cellular structural biology.

核磁共振核心 NMR Core 的总体目标是为 PCHPI 研究人员提供最先进的功能，用于原子 HIV-1 蛋白及其复合物的结构和动态的水平表征。为了实现这个目标， 我们将利用一流的仪器资源、创新的实验方案以及独特的 过去十年中发展起来的专业知识。具体目标旨在 i) 支持 PCHPI 项目和额外 中心合作者通过为 HIV-1 研究提供尖端的固态 NMR 能力，以及 ii) 开发 用于 HIV-1 研究的细胞内溶液和固态 19F NMR。 NMR Core 开发的新技术 将对 HIV-1 细胞结构生物学产生重大影响。