Core B: Functional Phenotyping Core

项目摘要/摘要：核心B：功能表型核心 在过去的20年里，一些关键的新疗法改变了癌症作为一种疾病的世界。 尽管取得了这些进展，但没有一种单一的治疗方法能保证治愈所有被诊断为这种疾病的人。 疾病。我们专注于白血病的Artnet中心的总体目标是在机械层面上了解如何 肿瘤在治疗压力下进化和适应，导致肿瘤失去控制，最终导致疾病 进步。具体地说，该应用程序询问影响的时间外在和内在因素 急性髓系白血病患者肿瘤演变及其对肿瘤微环境的影响在……里面 为了实现这一目标，我们组建了一支由白血病研究人员、临床医生和数据组成的非凡团队 有很长一段合作历史的科学家，他们在基础/机械和翻译研究之间架起了桥梁 以解决AML生物学以及这种生物学在药物治疗方面的变化。 功能表型核心将在标准化、优化 以及实施最先进的先进分子和功能分析。此外，FP团队将 假设对从其中每一个收集的所有数据进行异地、治理以及集成和标准化 Artnet中心使用的功能性基因组分析。这是一个好的执行的重要和必不可少的部分 多参数和多学科项目，这将允许提取最有意义的数据， 被交叉比较和解释。 功能表型核心的具体目标是：1)优化标准操作 程序，并对生物检疫的处理、跟踪和传播进行监督 AML患者；2)与所有Artnet领导和研究人员合作，制定和执行SOP 功能基因组平台，便于标准化数据采集和分析；3)作为数据 管家在治理、安全、存储、管理、标准、集成和 传播以支持中心内和中心间的分析和建模。 为了实现这些目标，功能表型核心将建立和实施SOP驱动 具有明确定义的里程碑和质量检查的工作流程，跨所有阶段和在此实施的分析 求婚。此外，FP核心将专注于所有检测的质量保证，并帮助在 需要意外故障排除的情况。总体而言，FP核心将作为所有研究人员的资源， 确保成功和及时地实现本申请的目标。

PROJECT SUMMARY/ABSTRACT: Core B: Functional Phenotyping Core In the last 20 years, a number of pivotal new treatments have changed the world of cancer as a disease. Despite these advances, not a single treatment offers a guaranteed cure to everyone who is diagnosed with this disease. The overall goal of our leukemia-focused ARTNet Center is to understand, on a mechanistic level, how tumors evolve and adapt under therapeutic pressure leading to loss of tumor control and ultimately disease progression. Specifically, this application interrogates the temporal extrinsic and intrinsic factors that influence tumor evolution and its influence on tumor microenvironment in patients with Acute Myeloid Leukemia (AML). In order to achieve this goal, we have assembled an exceptional team of leukemia researchers, clinicians and data scientists that have a long history of working together to bridge basic/mechanistic and translational research in order to tackle AML biology and how this biology changes upon drug treatments. The Functional Phenotyping core will support this ARTnet project in the standardization, optimization, and implementation of state-of-the art advanced molecular and functional assays. Additionally, the FP team will assume oversite, governance as well as integration and standardization of all data collected from each of these functional genomic assays used in this ARTNet Center. This is an important and essential part of a well-executed multiparametric and multidisciplinary project, which will allow for extraction of the most meaningful data that can be cross-compared and interpreted. The specific aims of the Functional Phenotyping core are to: 1) Optimize standard operating procedures and provide oversight for the processing, tracking, and dissemination of biospecimens from AML patients; 2) Work with all ARTNet leaders and researchers to develop and execute SOPs for functional genomic platforms to facilitate standardized data acquisition and analysis; 3) Serve as data stewards with regard to governance, security, storage, management, standards, integration, and dissemination to support intra- and inter-Center analyses and modeling. In order to achieve these aims, the Functional Phenotyping core will establish and implement SOP-driven work flows with well-defined milestones and quality check ins across all stages and assays implemented in this proposal. Further, the FP Core will focus on quality assurance for all assays and help with communication in the case that unforeseen troubleshooting is required. Overall, the FP core will serve as a resource for all researchers, making sure the goals of this application are achieved in successful and timely matter.