Core B: Functional Phenotyping Core

核心 B：功能表型核心

• 批准号: 10684103
• 负责人: Tothu Q Vu
• 金额: $ 13.01万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10684103
• 关键词: Acute Myelocytic Leukemia; Address; Architecture; Biological Assay; Biology; Cell Separation; Cell Survival; Clinic; Clinical; Clinical Data; Clinical Trials; Collection; Communication; Communities; Complex; Computerized Medical Record; Consent; Cryopreservation; Data; Data Analyses; Data Correlations; Data Scientist; Data Set; Dedications; Diagnosis; Disease; Disease Progression; Drug Combinations; Ensure; Evolution; Feeds; Goals; Immune signaling; Informed Consent; Infrastructure; Intrinsic factor; Malignant Neoplasms; Metadata; Methods; Modeling; Molecular; Patient Isolation; Patient-Focused Outcomes; Patients; Performance; Pharmacotherapy; Phenotype; Preparation; Procedures; Process; Protocols documentation; Quality Control; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Sampling; Security; Signal Transduction; Standardization; Stimulus; Structure; System; Therapeutic; Translational Research; Translations; Variant; Work; acquired drug resistance; cell preparation; data acquisition; data curation; data integration; data management; data standards; functional genomics; genomic platform; improved; large scale data; leukemia; multidisciplinary; neoplastic cell; novel; pressure; programs; quality assurance; success; therapy resistant; tumor; tumor microenvironment

PROJECT SUMMARY/ABSTRACT: Core B: Functional Phenotyping Core In the last 20 years, a number of pivotal new treatments have changed the world of cancer as a disease. Despite these advances, not a single treatment offers a guaranteed cure to everyone who is diagnosed with this disease. The overall goal of our leukemia-focused ARTNet Center is to understand, on a mechanistic level, how tumors evolve and adapt under therapeutic pressure leading to loss of tumor control and ultimately disease progression. Specifically, this application interrogates the temporal extrinsic and intrinsic factors that influence tumor evolution and its influence on tumor microenvironment in patients with Acute Myeloid Leukemia (AML). In order to achieve this goal, we have assembled an exceptional team of leukemia researchers, clinicians and data scientists that have a long history of working together to bridge basic/mechanistic and translational research in order to tackle AML biology and how this biology changes upon drug treatments. The Functional Phenotyping core will support this ARTnet project in the standardization, optimization, and implementation of state-of-the art advanced molecular and functional assays. Additionally, the FP team will assume oversite, governance as well as integration and standardization of all data collected from each of these functional genomic assays used in this ARTNet Center. This is an important and essential part of a well-executed multiparametric and multidisciplinary project, which will allow for extraction of the most meaningful data that can be cross-compared and interpreted. The specific aims of the Functional Phenotyping core are to: 1) Optimize standard operating procedures and provide oversight for the processing, tracking, and dissemination of biospecimens from AML patients; 2) Work with all ARTNet leaders and researchers to develop and execute SOPs for functional genomic platforms to facilitate standardized data acquisition and analysis; 3) Serve as data stewards with regard to governance, security, storage, management, standards, integration, and dissemination to support intra- and inter-Center analyses and modeling. In order to achieve these aims, the Functional Phenotyping core will establish and implement SOP-driven work flows with well-defined milestones and quality check ins across all stages and assays implemented in this proposal. Further, the FP Core will focus on quality assurance for all assays and help with communication in the case that unforeseen troubleshooting is required. Overall, the FP core will serve as a resource for all researchers, making sure the goals of this application are achieved in successful and timely matter.

项目总结/摘要：核心B：功能表型核心 在过去的20年里，一些关键的新疗法改变了癌症作为一种疾病的世界。 尽管有这些进步，但没有一种治疗方法能保证治愈所有被诊断患有这种疾病的人。 疾病我们以白血病为重点的ARTNet中心的总体目标是在机制层面上了解， 肿瘤在治疗压力下进化和适应，导致肿瘤失去控制并最终导致疾病 进展具体来说，这个应用程序询问时间的外在和内在因素，影响 急性髓系白血病（AML）患者的肿瘤演变及其对肿瘤微环境的影响。在 为了实现这一目标，我们组建了一个由白血病研究人员、临床医生和数据专家组成的特殊团队， 有着长期合作历史的科学家，在基础/机械和转化研究之间架起了桥梁， 以解决AML生物学以及这种生物学如何在药物治疗后发生变化。 功能表型核心将支持ARTnet项目的标准化，优化， 以及实施最先进的分子和功能分析。此外，FP团队将 承担从每个站点收集的所有数据的现场、治理以及集成和标准化 在这个ARTNet中心使用的功能基因组分析。这是一个执行良好的 多参数和多学科项目，这将有助于提取最有意义的数据， 进行交叉比较和解释。 功能表型核心的具体目标是：1）优化标准操作 程序，并监督生物标本的处理、跟踪和传播， AML患者; 2）与所有ARTNet领导人和研究人员合作，制定并执行 功能基因组平台，以促进标准化的数据采集和分析; 3）作为数据 在治理、安全、存储、管理、标准、集成和 传播，以支持中心内和中心间的分析和建模。 为了实现这些目标，功能表型核心将建立和实施SOP驱动的 工作流程，包括在此过程中实施的所有阶段和测定中定义明确的里程碑和质量检查 提议此外，FP Core将专注于所有检测的质量保证，并帮助在 需要进行不可预见的故障排除的情况。总体而言，FP核心将作为所有研究人员的资源， 确保成功和及时地实现本申请的目标。