Stimulation of Cervical Excitatory Interneurons to Restore Breathing After Chronic Cervical Spinal Cord Injury

刺激颈部兴奋性中间神经元以恢复慢性颈髓损伤后的呼吸

• 批准号: 10531878
• 负责人: Shekar N. Kurpad
• 金额: --
• 依托单位: CLEMENT J. ZABLOCKI VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10531878
• 关键词: Aging; Anatomy; Area; Biological; Brain Stem; Breathing; Cell Nucleus; Cervical; Cervical spinal cord injury; Cervical spinal cord structure; Cholera Toxin Protomer B; Chronic; Chronic Phase; Clinical; Communication; Communities; Data; Dependence; Development; Devices; Electromyography; Elements; Ensure; Face; Goals; Health; Impairment; Incidence; Individual; Injury; Interneurons; Knowledge; Life; Maintenance; Maps; Mechanical ventilation; Military Personnel; Modernization; Modification; Morbidity - disease rate; Motor Neurons; Motor output; Mus; Muscle; Neuronal Plasticity; Neurons; Neurosciences; Operative Surgical Procedures; Outcome; Output; Patients; Penetration; Pharmacogenetics; Phase; Play; Population; Publishing; Quality of life; Recovery; Recovery of Function; Respiration Disorders; Respiratory Center; Respiratory Diaphragm; Respiratory Insufficiency; Respiratory physiology; Role; Shapes; Spinal; Spinal Cord; Spinal cord injury; Spinal cord injury patients; Stress; Survivors; Synapses; Techniques; Technology; Therapeutic; Time; Tracer; Transgenic Mice; United States; United States Department of Veterans Affairs; Ventilator; Vertebral column; Veterans; Viral; Weaning; Whole Body Plethysmography; Work; designer receptors exclusively activated by designer drugs; disability; experimental study; health care delivery; high risk; improved; innovation; insight; military veteran; mortality; neural; neural network; novel; novel strategies; novel therapeutic intervention; prevent; respiratory; respiratory examination; sex; therapeutic target; therapy development; treatment strategy; ventilation

Dysfunctional breathing is a significant cause of morbidity and mortality after cervical SCI (cSCI). The ability to restore breathing in the chronic phase after cervical SCI (cSCI) is an overwhelming yet important goal. The diaphragm, the major inspiratory muscle, is innervated by phrenic motoneurons (PMNs) located in the cervical spinal cord (C3-C5). Cervical interneurons synapse onto PMNs and discharge in synchrony with phrenic inspir- atory output and modulate breathing. Premotor neurons within the rostral ventral respiratory group of neurons (rVRG) in the brainstem provide the main inspiratory drive to the spinal respiratory circuitry. Cervical spinal cord injury (SCI) disrupts the communication between rVRG and the spinal respiratory circuitry resulting in significant respiratory compromise. We recently demonstrated that pharmacogenetic stimulation of cervical excitatory in- terneurons (eINs) immediately after cSCI rescues breathing in mice; however, it is not known if this strategy will be effective in improving breathing in the chronic phase of cSCI. At the chronic stage, the respiratory network is known to undergo significant modifications. Moreover, our previous work in non-traumatic compressive injury to the cervical spinal cord demonstrated that cervical eINs play an integral role in promoting plasticity and main- taining ventilation despite a significant loss of PMNs. Given that cervical eINs integrate into the respiratory net- work, and they are necessary for the spontaneous respiratory recovery, they emerge as critical therapeutic tar- gets for respiratory recovery in the chronic phase after traumatic cSCI. We hypothesize that providing selec- tive excitatory input to surviving PMNs at the late stage of cSCI will enhance PMNs output and respiratory recovery. The first objective of this proposal aims to gain more significant insights into the status of the respira- tory neural network after chronic cSCI. The second objective of this proposal will examine a novel treatment strategy involving selective stimulation of cervical eINs to promote respiratory recovery in the chronic phase after cSCI. In aim 1, we will assess the survival of PMNs following C2 hemisection injury (C2Hx) using the monosynaptic retrograde tracer Cholera Toxin Subunit B to specifically track the PMNs. In the second part of Aim 1, we will simultaneously map the input-output connectivity of the cervical respiratory elements, the PMNs and the prephrenic cervical eINs, in the naïve-uninjured state and after cSCI. In Aim 2, we will selectively stimulate the cervical eINs in the region of the phrenic nuclei after chronic cSCI using the DREADD technology. This technique has innovative applications due to its ability to activate or silence neuronal populations non-invasively. The effect of stimulating cervical eINs on promoting respiratory recovery after chronic cSCI will be assessed using electro- myography and whole-body plethysmography. The expected outcomes of the proposed experiments are inno- vative as it will increase our knowledge of the spinal respiratory network after chronic cSCI and facilitate the development of a novel strategy to restore breathing in the chronic phase of cSCI. These results will have a meaningful positive impact on the health and survival of veterans with SCI, and the strategy examined here has the potential to be implemented for weaning patients off mechanical ventilation in the chronic phase after cSCI when promoting recovery has remained a daunting task. Additionally, SCI represents a significant focus area of the veterans, about 7% of those living with SCI in the United States have sustained their SCI while actively serving. Injuries are often due to penetrating injuries such as from gunshots or high-powered explosive devices. As such, military personnel with SCI face even more long-term disabilities than those endured by civilians with SCI, and the issues more pronounced in veterans with cSCI and respiratory dysfunctions.

呼吸功能障碍是颈脊髓损伤（cSCI）后发病和死亡的重要原因。的能力