ShEEP Request for Fusion Flow Cytometer and Cell Sorter

ShEEP 请求融合流式细胞仪和细胞分选仪

• 批准号: 9210823
• 负责人: Shekar N. Kurpad
• 金额: --
• 依托单位: CLEMENT J. ZABLOCKI VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9210823
• 关键词: Address; Adult; Aerosols; Animals; Area; Arthritis; Bacteria; Biological; Biological Markers; Blood; Bronchoalveolar Lavage; Calcium pyrophosphate deposition disease; Cell Culture Techniques; Cell Cycle; Cell Death; Cell Separation; Cell Survival; Cell Therapy; Cell surface; Cells; Characteristics; Chondrocytes; Collaborations; Collection; Complement; Computer Workstations; Computer software; Contract Services; Core Facility; Degenerative polyarthritis; Detection; Development; Devices; Diffusion Magnetic Resonance Imaging; Dyes; Ensure; Environment; Environmental air flow; Enzyme-Linked Immunosorbent Assay; Equipment; Excision; Exposure to; Face; Fee-for-Service Plans; Fees; Flow Cytometry; Funding; General Population; Goals; Grant; HIV-1; Healthcare; Housing; Human; Human T-lymphotropic virus 2; Image; Image Analysis; Immune response; In Vitro; Inflammation; Institution; Ischemia; Journals; Label; Laboratories; Lasers; Lesion; Life; Location; Lung Transplantation; Magnetic Resonance Imaging; Maintenance; Malignant Epithelial Cell; Marketing; Medical center; Mesenchymal Stem Cells; Methods; Microscope; Mission; Mitochondria; Modeling; Molecular; Oxidative Stress; Oxidative Stress Induction; Performance; Play; Population; Power Sources; Preparation; Procedures; Production; Property; Proteins; Public Health; Publishing; Rattus; Reactive Oxygen Species; Reader; Rectal Cancer; Reperfusion Injury; Reperfusion Therapy; Research; Research Personnel; Research Project Grants; Resources; Risk; Role; Safety; Sample Size; Sampling; Serotonin Receptors 5-HT4; Signal Transduction; Site; Sorting - Cell Movement; Source; Spinal cord injury; Staining method; Stains; Sterility; Structure of parenchyma of lung; Surface; System; Therapeutic Intervention; Time; Tissues; Training; Transitional Cell Carcinoma; Translating; Transport Process; Traumatic Brain Injury; Travel; Veterans; Viral Physiology; Virus; Western Blotting; Wisconsin; Work; antibody conjugate; base; chemokine; cost; cytokine; cytotoxicity; density; fluorophore; improved; in vivo; injured; instrument; lung injury; lung ischemia; medical schools; prevent; programs; regenerative; research study; tool; treatment effect

The primary justification for the requested system is the current lack of flow cytometry and cell sorting at the Milwaukee VA despite a high demand for such procedures. The requested system is a BD FACSARIA Fusion Special Order flow cytometry and cell sorting system with all components necessary to execute multicolor flow cytometry and cell sorting experiments. The instrument will consist of five lasers, which allows for detection of 18 fluorescent parameters. This will enable researchers at the Milwaukee VA to detect a large variety of cellular markers in the same cell. This instrument is produced by the market leader BD. VA researchers have previously used flow cytometry and cell sorting at the core facilities of the Medical College of Wisconsin. The same instruments and software are represented at the core facilities, which would allow researchers at the VA to continue their experiments without technical adjustment. The research projects which would strongly benefit from the presence of a flow cytometer and cell sorter at the VA include a variety of conditions relevant to both Veterans and the general population. Dr. Kurpad is using diffusion tensor imaging (DTI) analysis of the injured spinal cord at the lesion site and proximal to the lesion. Flow cytometry will be used to verify imaging results and to identify and correlate cell activation, cell death and cytotoxicity to the DTI results. Dr. Jacobs is investigating ischemia-reperfusion lung injuries, a commonly encountered condition after lung transplantation. Flow cytometry will be performed on cells derived from bronchoalveolar lavage and on enzymatically digested lung tissue and will provide information about cell signaling and mitochondrial properties. Furthermore, the result of therapeutic interventions will be assessed by flow cytometry. Dr. Rosenthal's research is focused on a form of degenerative arthritis, the calcium pyrophosphate deposition disease. The cell sorter would be used to sort populations of successfully transfected chondrocytes which can then be used for further experiments. Dr. See's project addresses the induction of oxidative stress in urothelial carcinoma cells by BCG bacteria. This oxidative stress is a critical component for the antitumor activity of BCG. Flow cytometry will be used to detect treatment effects in terms of cytotoxicity and cell death. Dr. Kroner-Milsch is investigating the role of inflammation after spinal cord injury (SCI). She aims to reduce secondary damage by specifically blocking cytokines and chemokines which are upregulated following SCI. Cells derived from injured tissue will be analyzed for production of cytokines and reactive oxygen species. Dr. Glavaski-Joksimovic's research focus is the neuroprotective effect of adult mesenchymal stem cells (MSC) after traumatic brain injury (TBI). Flow cytometry and cell sorting will be used to study and sort different MSC subpopulations and compare their effects in TBI models. Dr. Beilke is investigating the role of HTLV-2 virus as a modulator of immune response. Tax2, a HTLV-2 protein, is a strong chemokine regulator and plays a role in anti-viral activity against HIV-1. Flow cytometry is frequently applied in his studies to detect expression of surface molecules in specific cell populations. Dr. Budde's research focus is the development of noninvasive MRI biomarkers for SCI and TBI. A flow cytometry and cell sorting instrument will be necessary for cell based therapy studies with a high promise of neuroprotective and regenerative effects. Dr. Ridolfi investigates the role of 5-HT3 and 5-HT4 receptors after rectal cancer resection. Flow cytometry will be used to assess expression density in his rat model of parasympathetic damage. In summary, access to a flow cytometer and cell sorter at the Milwaukee VA would accelerate research projects currently done at the VA and open new research opportunities for VA investigators.

要求该系统的主要理由是目前缺乏流式细胞术和细胞分选