Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Mapping of the Human Kidney

范德比尔特大学生物分子多模态成像中心进行人体肾脏 3 维绘图

• 批准号: 10530865
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 199.86万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10530865
• 关键词: 3-Dimensional; Academic Medical Centers; Age; Anatomy; Applications Grants; Atlases; Biomedical Research; Cells; Clinical; Clinical Data; Collaborations; Collection; Communities; Cooperative Human Tissue Network; Data; Data Analyses; Data Set; Development; Dimensions; Disease; Ensure; Environment; Eye; Freezing; Funding; Generations; Goals; Health; Histologic; Histology; Human; Human BioMolecular Atlas Program; Image; Imaging technology; Immunofluorescence Microscopy; Infrastructure; Institutes; International; Kidney; Knowledge; Laboratories; Lipids; Mass Spectrum Analysis; Medical Imaging; Medical center; Medicine; Microscopy; Mission; Modality; Molecular; Molecular Analysis; Monitor; Multimodal Imaging; Nephrectomy; Netherlands; Organ; Pancreas; Participant; Performance; Phenotype; Physicians; Positioning Attribute; Procedures; Process; Proteins; Proteomics; Quality Control; RNA; Race; Reproducibility; Research; Research Personnel; Resolution; Resources; Sampling; Scientist; Selection Criteria; Small Nuclear RNA; Specimen; Stains; Standardization; Surface; System; Techniques; Technology; Testing; Three-Dimensional Imaging; Time; Tissue Donors; Tissues; Transcript; Transplantation; United States National Institutes of Health; Universities; Work; body system; cell type; chemical fixation; demographics; ex vivo imaging; human tissue; imaging facilities; imaging modality; improved; in vivo; innovation; insight; mass spectrometric imaging; member; multidisciplinary; multimodal data; multimodality; novel; preservation; programs; quality assurance; sex; single-cell RNA sequencing; tissue mapping; transcriptome sequencing; transcriptomics

PROJECT SUMMARY – Overall This application proposes the continuation of a Tissue Mapping Center (TMC) for the human kidney within the Human BioMolecular Atlas Program (HuBMAP). The mission of the proposed TMC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3- dimensional molecular atlases of the human kidney. This TMC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University, the world-class clinical environment of the Vanderbilt University Medical Center, and the advanced biocomputational infrastructure available to the TMC through the Data Analysis Core laboratories at Vanderbilt University and the Delft University of Technology, Netherlands to create a capability to molecularly characterize human tissues in 3-dimensions at a level of understanding unrivaled by current technologies. The innovative aspect of the proposed TMC is the integration of imaging mass spectrometry, highly multiplexed immunofluorescence microscopy, autofluorescence microscopy, stained microscopy, spatial transcriptomics, spatial proteomics, and single-cell RNA-seq to create comprehensive 2-D and 3-D molecular and cellular atlases of the human kidney. Through segmentation and cell type analysis determined by classical markers, we will correlate our molecular and clinical information to the existing knowledge of the kidney to better define the normal phenotypes across a range of diverse samples. The application of this multimodal pipeline to the kidney will provide a new paradigm of understanding the normal state of this organ across multiple dimensions, both molecular (e.g., lipids, metabolites, proteins, and transcripts) and spatial (e.g., whole organs to single cells). As a HuBMAP participant, the molecular atlases produced by this TMC will be disseminated to the community and to collaborators to generate new hypotheses regarding the function of this important organ system, enabling new insight into human health and disease. This multidisciplinary effort requires the creation and integration of three capabilities to 1) procure and manage human tissue specimens, 2) determine and mitigate non-biological pre- analytical factors, and 3) acquire, process, and disseminate multimodal 3-dimensional imaging and large-scale omics data. The goal of the proposed kidney TMC is to create atlases for community users, physicians, and researchers to navigate across the anatomical and molecular landscape of the kidney, generate novel hypotheses, and tackle meaningful biomedical research questions.

项目摘要 - 总体 该应用建议在人类肾脏中延续组织映射中心（TMC） 人类生物分子图集计划（Hubmap）。拟议的TMC的任务是建立一个平台 用于成像和分子分析的集成技术，可实现综合3-的构建 人肾脏的尺寸分子图谱。该TMC将利用群众的独特资源 光谱研究中心和国家成像质谱的国家研究资源 范德比尔特大学，范德比尔特大学医学中心的世界一流临床环境， TMC可通过数据分析核心实验室提供的高级生物计算基础架构 范德比尔特大学和荷兰代尔夫特理工大学创造了分子的能力 在三维中表征人类组织，在当前技术无与伦比的理解水平上。这 提出的TMC的创新方面是成像质谱法的整合，高度多路复用 免疫荧光显微镜，自荧光显微镜，染色显微镜，空间转录组学， 空间蛋白质组学以及单细胞RNA-Seq，以创建全面的2-D和3-D分子和细胞图谱 人类肾脏。通过通过经典标记确定的分割和细胞类型分析，我们将 将我们的分子和临床信息与肾脏的现有知识相关联，以更好地定义正常 各种潜水员样品的表型。将此多模式管道应用于肾脏将 提供一个新的范式，以了解跨多个维度的正常状态 分子（例如脂质，代谢物，蛋白质和转录本）和空间（例如，整个器官到单细胞）。作为 Hubmap参与者，该TMC生产的分子图谱将被传播到社区， 与合作者生成有关此重要器官系统功能的新假设 深入了解人类健康和疾病。这种多学科的努力需要创建和整合三个 1）采购和管理人体组织标本的能力，2） 分析因素以及3）获取，过程和传播多模式3维成像和大规模 OMICS数据。拟议的肾脏TMC的目标是为社区用户，医生和 研究人员在肾脏的解剖学和分子景观中导航，产生了新颖 假设，并解决有意义的生物医学研究问题。