权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Mapping of the Human Kidney

范德比尔特大学生物分子多模态成像中心进行人体肾脏 3 维绘图

基本信息

批准号：
10530865
负责人：
RICHARD M CAPRIOLI
金额：
$ 199.86万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-15 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10530865
关键词：
3-Dimensional Academic Medical Centers Age Anatomy Applications Grants Atlases Biomedical Research Cells Clinical Clinical Data Collaborations Collection Communities Cooperative Human Tissue Network Data Data Analyses Data Set Development Dimensions Disease Ensure Environment Eye Freezing Funding Generations Goals Health Histologic Histology Human Human BioMolecular Atlas Program Image Imaging technology Immunofluorescence Microscopy Infrastructure Institutes International Kidney Knowledge Laboratories Lipids Mass Spectrum Analysis Medical Imaging Medical center Medicine Microscopy Mission Modality Molecular Molecular Analysis Monitor Multimodal Imaging Nephrectomy Netherlands Organ Pancreas Participant Performance Phenotype Physicians Positioning Attribute Procedures Process Proteins Proteomics Quality Control RNA Race Reproducibility Research Research Personnel Resolution Resources Sampling Scientist Selection Criteria Small Nuclear RNA Specimen Stains Standardization Surface System Techniques Technology Testing Three-Dimensional Imaging Time Tissue Donors Tissues Transcript Transplantation United States National Institutes of Health Universities Work body system cell type chemical fixation demographics ex vivo imaging human tissue imaging facilities imaging modality improved in vivo innovation insight mass spectrometric imaging member multidisciplinary multimodal data multimodality novel preservation programs quality assurance sex single-cell RNA sequencing tissue mapping transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY – Overall This application proposes the continuation of a Tissue Mapping Center (TMC) for the human kidney within the Human BioMolecular Atlas Program (HuBMAP). The mission of the proposed TMC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3- dimensional molecular atlases of the human kidney. This TMC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University, the world-class clinical environment of the Vanderbilt University Medical Center, and the advanced biocomputational infrastructure available to the TMC through the Data Analysis Core laboratories at Vanderbilt University and the Delft University of Technology, Netherlands to create a capability to molecularly characterize human tissues in 3-dimensions at a level of understanding unrivaled by current technologies. The innovative aspect of the proposed TMC is the integration of imaging mass spectrometry, highly multiplexed immunofluorescence microscopy, autofluorescence microscopy, stained microscopy, spatial transcriptomics, spatial proteomics, and single-cell RNA-seq to create comprehensive 2-D and 3-D molecular and cellular atlases of the human kidney. Through segmentation and cell type analysis determined by classical markers, we will correlate our molecular and clinical information to the existing knowledge of the kidney to better define the normal phenotypes across a range of diverse samples. The application of this multimodal pipeline to the kidney will provide a new paradigm of understanding the normal state of this organ across multiple dimensions, both molecular (e.g., lipids, metabolites, proteins, and transcripts) and spatial (e.g., whole organs to single cells). As a HuBMAP participant, the molecular atlases produced by this TMC will be disseminated to the community and to collaborators to generate new hypotheses regarding the function of this important organ system, enabling new insight into human health and disease. This multidisciplinary effort requires the creation and integration of three capabilities to 1) procure and manage human tissue specimens, 2) determine and mitigate non-biological pre- analytical factors, and 3) acquire, process, and disseminate multimodal 3-dimensional imaging and large-scale omics data. The goal of the proposed kidney TMC is to create atlases for community users, physicians, and researchers to navigate across the anatomical and molecular landscape of the kidney, generate novel hypotheses, and tackle meaningful biomedical research questions.

项目概述-整体