Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Mapping of the Human Kidney

范德比尔特大学生物分子多模态成像中心进行人体肾脏 3 维绘图

• 批准号: 10701827
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 221.16万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10701827
• 关键词: 3-Dimensional; Academic Medical Centers; Age; Anatomy; Applications Grants; Atlases; Biomedical Research; Cells; Clinical; Clinical Data; Collaborations; Collection; Communities; Cooperative Human Tissue Network; Data; Data Analyses; Data Set; Development; Dimensions; Disease; Ensure; Environment; Eye; Freezing; Funding; Generations; Goals; Health; Histologic; Histology; Human; Human BioMolecular Atlas Program; Image; Imaging technology; Immunofluorescence Microscopy; Infrastructure; International; Ischemia; Kidney; Knowledge; Laboratories; Lipids; Maps; Mass Spectrum Analysis; Medical Imaging; Medical center; Medicine; Microscopy; Mission; Modality; Molecular; Molecular Analysis; Monitor; Multimodal Imaging; National Center for Research Resources; Nephrectomy; Netherlands; Organ; Pancreas; Participant; Performance; Phenotype; Physicians; Positioning Attribute; Procedures; Process; Proteins; Proteomics; Quality Control; RNA; Race; Reproducibility; Research; Research Personnel; Resolution; Resources; Sampling; Scientist; Selection Criteria; Specimen; Stains; Standardization; Surface; System; Techniques; Technology; Testing; Time; Tissue Donors; Tissues; Transcript; Transplantation; United States National Institutes of Health; Universities; Work; biocomputing; body system; cell type; chemical fixation; data integration; demographics; ex vivo imaging; human tissue; imaging facilities; imaging modality; improved; in vivo; innovation; insight; mass spectrometric imaging; member; multidisciplinary; multimodal data; multimodality; novel; preservation; programs; quality assurance; sex; single nucleus RNA-sequencing; single-cell RNA sequencing; tissue mapping; transcriptome sequencing; transcriptomics

PROJECT SUMMARY – Overall This application proposes the continuation of a Tissue Mapping Center (TMC) for the human kidney within the Human BioMolecular Atlas Program (HuBMAP). The mission of the proposed TMC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3- dimensional molecular atlases of the human kidney. This TMC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University, the world-class clinical environment of the Vanderbilt University Medical Center, and the advanced biocomputational infrastructure available to the TMC through the Data Analysis Core laboratories at Vanderbilt University and the Delft University of Technology, Netherlands to create a capability to molecularly characterize human tissues in 3-dimensions at a level of understanding unrivaled by current technologies. The innovative aspect of the proposed TMC is the integration of imaging mass spectrometry, highly multiplexed immunofluorescence microscopy, autofluorescence microscopy, stained microscopy, spatial transcriptomics, spatial proteomics, and single-cell RNA-seq to create comprehensive 2-D and 3-D molecular and cellular atlases of the human kidney. Through segmentation and cell type analysis determined by classical markers, we will correlate our molecular and clinical information to the existing knowledge of the kidney to better define the normal phenotypes across a range of diverse samples. The application of this multimodal pipeline to the kidney will provide a new paradigm of understanding the normal state of this organ across multiple dimensions, both molecular (e.g., lipids, metabolites, proteins, and transcripts) and spatial (e.g., whole organs to single cells). As a HuBMAP participant, the molecular atlases produced by this TMC will be disseminated to the community and to collaborators to generate new hypotheses regarding the function of this important organ system, enabling new insight into human health and disease. This multidisciplinary effort requires the creation and integration of three capabilities to 1) procure and manage human tissue specimens, 2) determine and mitigate non-biological pre- analytical factors, and 3) acquire, process, and disseminate multimodal 3-dimensional imaging and large-scale omics data. The goal of the proposed kidney TMC is to create atlases for community users, physicians, and researchers to navigate across the anatomical and molecular landscape of the kidney, generate novel hypotheses, and tackle meaningful biomedical research questions.

项目概要-总体 本申请提出了在本发明的范围内继续人类肾脏的组织映射中心（TMC）。 人类生物分子图谱计划（HuBMAP）。拟议TMC的使命是建立一个平台， 用于成像和分子分析的集成技术，能够构建全面的3- 人类肾脏的三维分子图谱。该TMC将利用大众的独特资源 光谱研究中心和国家成像质谱研究资源， 范德比尔特大学，范德比尔特大学医学中心的世界级临床环境，以及 先进的生物计算基础设施，通过数据分析核心实验室提供给TMC， 荷兰范德比尔特大学和德尔夫特理工大学合作， 以当前技术无法比拟的理解水平在三维中表征人体组织。的 所提出的TMC的创新方面是成像质谱的集成，高度多路复用， 免疫荧光显微术，自体荧光显微术，染色显微术，空间转录组学， 空间蛋白质组学和单细胞RNA-seq，以创建全面的二维和三维分子和细胞图谱 人类肾脏的一部分通过经典标记物确定的分割和细胞类型分析， 将我们的分子和临床信息与肾脏的现有知识相关联，以更好地定义正常的肾脏。 在一系列不同的样品中进行表型分析。将这种多模式管道应用于肾脏将 提供了一个新的范式，可以从多个维度理解这个器官的正常状态， 分子（例如，脂质、代谢物、蛋白质和转录物）和空间（例如，整个器官到单个细胞）。作为 作为HuBMAP的参与者，该TMC制作的分子地图集将分发给社区， 合作者产生关于这个重要器官系统功能的新假设，使新的 深入了解人类健康和疾病。这种多学科的努力需要建立和整合三个 能够1）获取和管理人体组织标本，2）确定和减轻非生物预处理， 分析因素，以及3）获取，处理和传播多模态三维成像和大规模 组学数据。拟议的肾脏TMC的目标是为社区用户、医生和 研究人员在肾脏的解剖和分子景观中导航，产生新的 假设，并解决有意义的生物医学研究问题。

项目成果

Prospective on Imaging Mass Spectrometry in Clinical Diagnostics.

• DOI: 10.1016/j.mcpro.2023.100576
• 发表时间: 2023-09
• 期刊: MOLECULAR & CELLULAR PROTEOMICS
• 影响因子: 7
• 作者: Moore, Jessica L.;Patterson, Nathan Heath;Norris, Jeremy L.;Caprioli, Richard M.
• 通讯作者: Caprioli, Richard M.