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Biomolecular Multimodal Imaging Center: 3-Dimensional Tissue Mapping of the Human Pancreas and Eye

生物分子多模态成像中心：人类胰腺和眼睛的 3 维组织图谱

基本信息

批准号：
10704485
负责人：
RICHARD M CAPRIOLI
金额：
$ 193.79万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-30 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10704485
关键词：
3-Dimensional Academic Medical Centers Alabama Applications Grants Atlases Cells Clinical Clinical Data Collaborations Collection Communities Data Data Analyses Data Set Detection Development Diabetes Mellitus Dimensions Disease Ensure Environment Excision Eye Florida Foundations Freezing Funding Future Generations Health Histologic Histology Human Human BioMolecular Atlas Program Image Imaging technology Immunofluorescence Immunologic Infrastructure Kidney Laboratories Lipids Mass Spectrum Analysis Medical Imaging Medical center Microscopy Mission Modality Molecular Molecular Analysis Monitor Multimodal Imaging National Center for Research Resources Netherlands Normal tissue morphology Operative Surgical Procedures Optical Coherence Tomography Organ Organ Procurements Pancreas Participant Performance Physicians Positioning Attribute Preparation Procedures Process Proteins Proteomics Quality Control Reproducibility Research Research Personnel Resources Sampling Scientist Source Specimen Stains Standardization Surface System Technical Expertise Techniques Technology Testing Tissues United States National Institutes of Health Universities biocomputing body system cell type chemical fixation computing resources data acquisition data integration ex vivo imaging experience human tissue imaging facilities imaging modality in vivo indexing innovation insight mass spectrometric imaging member metabolomics multidisciplinary multimodality preservation programs quality assurance single-cell RNA sequencing tissue mapping

项目摘要

PROJECT SUMMARY – Overall. This application proposes the founding of a Tissue Mapping Center (TMC) for the human eye and pancreas within the Human BioMolecular Atlas Program (HuBMAP). The mission of the proposed TMC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3-dimensional molecular atlases of human eye and pancreas. This TMC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University, the world-class clinical environment of the Vanderbilt University Medical Center, the distinguished expertise of Organ Specific Project team leaders in their respective fields and their vast experience in organ procurement and management, and the advanced biocomputational infrastructure available to the TMC through the Data Analysis Core laboratories at Vanderbilt University and the Delft University of Technology, Netherlands to create a capability to molecularly characterize human tissues in 3-dimensions at a level of understanding unrivaled by current technologies. The innovative aspects of the proposed TMC are (1) the integration of imaging mass spectrometry, co-detection by indexing (CODEX), stained microscopy, and autofluorescence microscopy into 3-dimensional molecular atlases, (2) whole organ imaging of the eye, and (3) multi-organ atlases from the same donors through a proposed collaboration with the currently funded University of Florida TMC. The application of the proposed platform to organ-specific projects in pancreas and eye will provide a new paradigm of understanding the normal state of these organs across vast scales, both molecular (e.g., lipids, metabolites, and proteins) and spatial (e.g., whole organs to single cells). Furthermore, the involvement of these organs in diabetes, along with organs currently funded by HuBMAP such as kidney and vasculature, will lay an important foundation for future studies striving to understand the progression of diabetes. As a HuBMAP participant, the molecular atlases produced by this TMC will be disseminated to collaborators to generate new hypotheses regarding the function of these important organ systems, enabling new insight into human health and disease. This multidisciplinary effort requires the creation and integration of three capabilities to 1) procure and manage human tissue specimens, 2) determine and mitigate non-biological pre-analytical factors, and 3) acquire, process, and disseminate multimodal 3-dimensional imaging and large-scale omics data.

项目总结-总体。该申请提出建立组织映射中心（TMC）人类生物分子图谱计划（HuBMAP）中的人眼和胰腺。联合国的使命拟议的TMC是建立一个成像和分子分析集成技术平台，人眼和胰腺三维分子图谱的构建。该TMC将利用质谱研究中心和国家研究中心的独特资源，范德比尔特大学的成像质谱资源，世界一流的临床环境，范德比尔特大学医学中心，器官特异性项目团队领导的杰出专业知识，他们各自的领域和丰富的经验，在器官采购和管理，和先进的通过位于范德比尔特的数据分析核心实验室，TMC可以使用生物计算基础设施大学和荷兰德尔夫特理工大学合作，以当前技术无法比拟的理解水平在三维中表征人体组织。的所提出的TMC的创新方面是（1）成像质谱的集成，索引（CODEX），染色显微镜和自体荧光显微镜到三维分子图集，（2）眼睛的整个器官成像，以及（3）通过建议与目前资助的佛罗里达大学TMC合作。建议的应用胰腺和眼睛器官特异性项目的平台将提供一个新的范式，这些器官在大尺度上的正常状态，分子（例如，脂质、代谢物和蛋白质）和空间的（例如，整个器官到单个细胞）。此外，这些器官参与糖尿病，沿着目前由HuBMAP资助的器官，如肾脏和血管，将为未来的研究努力了解糖尿病的进展。作为HuBMAP参与者，该TMC制作的地图集将分发给合作者，以产生关于这些重要器官系统的功能，使新的洞察人类健康和疾病。这多学科的努力需要建立和整合三种能力：1）采购和管理人体组织样本，2）确定和减轻非生物预分析因素，以及3）获取，处理和传播多模式三维成像和大规模组学数据。