Organ Specific Project - Heart

项目总结-心脏OSP 人类心脏对我们的生存和健康至关重要，它具有显著的解剖学，细胞和功能 异质性心脏的四个腔室，以及专门的动脉，静脉，瓣膜和传导 细胞，执行不同但基本的生理功能。一个重要的知识缺口是，不同的细胞 分子特征、空间分布和相互作用以及功能状态仍然知之甚少 单细胞水平。心脏器官特异性项目（OSP）的目标是解决这一知识差距， 生成正常人心脏的高质量单细胞分辨率纵向成像和多组学数据 在整个人类的生命周期中。为了实现这一目标，我们将生成：1）人类心脏图谱。空间 解决的地图集将提供一个高度用户友好的，公开的，可搜索的数据库， 对正常人心脏进行全面的多组学、单细胞分析。分子数据将丰富 注释有额外的临床和人口统计学数据。2)计算方法。除了地图集数据， 在这个项目中开发的关键计算工具和管道将可用于研究 社区这些包括用于处理多组学和成像数据的方法和管道，细胞的推断， 特异性调节和信号通路，中尺度成像和分子成像特征的相关性， 以及用于高度复杂数据的查询、探索和可视化的数据库算法。3)获得 用于后续研究的生物标本。本项目中收集的生物标本将储存起来， 生物医学研究界。这些包括新鲜冷冻和固定的标本和组织切片。在 总之，心脏OSP将广泛影响整个研究界，并启动基础科学， 医学发现的基础上的关键分子电路的复杂理解， 人类心脏的发育和衰老。

PROJECT SUMMARY – HEART OSP The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell’s molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we will generate: 1) Human heart atlases. Spatially resolved atlases will provide a highly user friendly, publicly available, searchable database of the most comprehensive multi-omic, single cell analysis of the normal human hearts. Molecular data will be richly annotated with additional clinical and demographic data. 2) Computational methods. In addition to the atlas data, the critical computational tools and pipelines developed in this project will be available to the research community. These include methods and pipelines for processing multi-omics and imaging data, inference of cell- specific regulatory and signaling pathways, correlation of mesoscale imaging and molecular imaging features, as well as database algorithms for the query, exploration and visualization of highly complex data. 3) Access to biospecimens for follow-up studies. Biospecimens collected in this project will be banked and made available to the biomedical research community. These include freshly frozen and fixed specimens and tissue sections. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.