Organ Specific Project - Heart

项目总结-心脏器官特定项目 人类心脏对我们的生存和健康至关重要，它呈现出非凡的解剖学，细胞学和生物学特征。 功能异质性心脏的四个腔室以及专门的动脉、静脉、瓣膜和 传导细胞，执行不同但基本的生理功能。一个重大的知识差距是， 不同细胞的分子特征、空间分布和相互作用以及功能状态仍然很少 在单细胞水平上理解。心脏器官特异性项目（OSP）的目标就是解决这个问题。 产生高质量、单细胞分辨率、纵向成像和多组学数据， 正常人的心脏。为实现这一目标，我们提出以下三点建议： 具体目标：1）完善生物标本处理、多组学和成像分析的方案，并定义 个体间的变异性。2)采购、存档和注释 高质量的正常心脏样本。我们建立了一个精简的 采购和生物储存库基础设施，以支持我们的核心OSP。我们会得到正常的心脏和骨骼 来自5个不同年龄段的同一个捐赠者。3)在空间和 使用一组强大的， 可扩展的成像和单细胞组学分析。总之，心脏OSP将广泛影响整个 研究社区和快速启动基础科学和医学发现的基础上，一个复杂的 了解人类心脏发育和衰老的关键分子回路。

PROJECT SUMMARY – HEART ORGAN SPECIFIC PROJECT The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell's molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we propose the following three specific aims: 1) To refine protocols of biospecimen processing, multiomics and imaging assays and define inter-individual variability using our existing banked normal human hearts. 2) To procure, archive and annotate high-quality normal heart samples across the entire human life span. We have established a streamlined procurement and biorepository infrastructure to support our heart OSP. We will procure normal heart and bone from the same donor of 5 different age groups across the entire human life span. 3) To spatially and quantitatively profile normal heart specimens across the entire human lifespan using a set of robust and scalable imaging and single-cell omics assays. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.