Organ Specific Project - Heart

器官特定项目 - 心脏

• 批准号: 10251352
• 负责人: Liming Pei
• 金额: $ 5.96万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10251352
• 关键词: 3-Dimensional; Address; Age; Aging; Anatomy; Archives; Arteries; Atlases; Basic Science; Biological Assay; Biology; Bone Tissue; Cardiac; Cell Nucleus; Cells; Chromatin; Clinical; Collection; Communities; Consent Forms; Data; Data Analyses; Detection; Development; Doctor of Medicine; Doctor of Philosophy; Epigenetic Process; Ethnic Origin; Female; Fluorescent in Situ Hybridization; Future; Genetic Transcription; Genomics; Goals; Growth; Health; Heart; Heterogeneity; Histologic; Hour; Human; Human BioMolecular Atlas Program; Image; Infrastructure; Knowledge; Leadership; Limes; Location; Longevity; Medical; Metadata; Molecular; Molecular Profiling; Multiomic Data; Organ; Organ Procurements; Persons; Physiological; Pilot Projects; Proteomics; Protocols documentation; Research; Research Personnel; Resolution; Sampling; Series; Small Nuclear RNA; Spatial Distribution; Specimen; Three-Dimensional Imaging; Tissue Banks; Transposase; Veins; age group; base; biobank; bone; cell type; data sharing; design; epigenome; experience; genomic data; indexing; inter-individual variation; male; member; metabolomics; microCT; multiple omics; serial imaging; sex; transcriptome; transcriptome sequencing; transcriptomics

PROJECT SUMMARY – HEART ORGAN SPECIFIC PROJECT The human heart is vital for our survival and health, and it presents remarkable anatomical, cellular and functional heterogeneity. The four chambers of the heart, together with specialized arteries, veins, valves and conduction cells, perform distinct yet essential physiological functions. A significant gap of knowledge is that different cell's molecular signature, spatial distribution and interactions, and functional state remain little understood at the single-cell level. The goal of the heart Organ-Specific Project (OSP) is to address this knowledge gap and generate high quality, single-cell resolution, longitudinal imaging and multiomics data of normal human hearts across the entire human lifespan. To achieve this goal, we propose the following three specific aims: 1) To refine protocols of biospecimen processing, multiomics and imaging assays and define inter-individual variability using our existing banked normal human hearts. 2) To procure, archive and annotate high-quality normal heart samples across the entire human life span. We have established a streamlined procurement and biorepository infrastructure to support our heart OSP. We will procure normal heart and bone from the same donor of 5 different age groups across the entire human life span. 3) To spatially and quantitatively profile normal heart specimens across the entire human lifespan using a set of robust and scalable imaging and single-cell omics assays. In summary, the heart OSP will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of human heart.

项目摘要-心脏器官专用项目 人类的心脏对我们的生存和健康至关重要，它在解剖学、细胞学和 功能异构性。心脏的四个腔，连同专门的动脉、静脉、瓣膜和 传导细胞发挥着独特而基本的生理功能。知识上的一个重大差距是 不同细胞的分子特征、空间分布和相互作用以及功能状态仍然很少 在单细胞水平上被理解。心脏器官专属项目(OSP)的目标就是解决这个问题 知识缺口，并生成高质量的单细胞分辨率、纵向成像和多组学数据 人类一生中的正常心脏。为了实现这一目标，我们提出了以下三点建议 具体目标：1)完善生物样品处理、多组学和成像分析的方案，并确定 使用我们现有的银行正常的人类心脏进行个体间的变异。2)采购、存档和批注 覆盖整个人类生命周期的高质量正常心脏样本。我们已经建立了一个精简的 采购和生物库基础设施，以支持我们的核心OSP。我们将获得正常的心脏和骨骼 来自同一捐赠者的5个不同年龄段，贯穿整个人类寿命。3)在空间上和 使用一组健壮的和 可扩展成像和单细胞组学分析。总而言之，心脏OSP将广泛影响整个 研究社区并启动基础科学和医学发现，基于复杂的 了解人类心脏发育和衰老的关键分子回路。