Modern and secure freezing, storage, and distribution of mouse strains at CWRU

摘要 凯斯转基因和靶向机构是为美国国立卫生研究院资助的唯一转基因核心 俄亥俄州东北部的研究人员。核心已经创建并冷冻保存了小鼠的遗传模型 已经有二十多年了。这些老鼠模型已被当地研究人员使用，并 世界各地的研究人员研究癌症、囊性纤维化、神经变性、病毒 免疫学、骨骼发生学、心血管疾病和许多其他疾病和基础 生物过程。基因编辑增加了核心已经能够实现的模型的数量 去创造。现在，我们所服务的NIH资助的研究人员比以往任何时候都更需要这些菌株的支持 上传、存档以供将来检索，或分发给他们的合作者和其他科学家 冷冻的精子或胚胎。此应用程序请求为设备提供资金， 对核心的老鼠品系进行现代化的冷冻保存，使其更具成本效益，增加 能力，并使业务符合当前的最佳做法。核心需要这个 现代化，以满足当地研究人员、研究中心和新技术的需求 在基因导向疗法的翻译和研究方面的机构研究倡议 微生物组。

Abstract The Case Transgenic and Targeting Facility is the sole transgenic core serving NIH-funded researchers in Northeast Ohio. The core has created and cryopreserved mouse genetic models for over twenty years. These mouse models have been used by local researchers and researchers around the world to study cancer, cystic fibrosis, neurodegeneration, viral immunology, skeletogenesis, cardiovascular disease, and many other diseases and fundamental biological processes. Gene editing increased the number of models that the core has been able to create. Now more than ever, the NIH-funded researchers we serve need these strains backed up, archived for future retrieval, or distributed to their collaborators and other scientists as cryopreserved sperm or embryos. This application requests funding for equipment which will modernize the core's mouse strain cryopreservation, make it more cost-efficient, increase capacity and bring the operation in line with current best practices. The core needs this modernization to meet the demand from local researchers, research centers, and from new institutional research initiatives in translation of gene-directed therapies and in studies of the microbiome.