Modern and secure freezing, storage, and distribution of mouse strains at CWRU

CWRU 对小鼠品系进行现代化、安全的冷冻、储存和分配

基本信息

批准号：
10531722
负责人：
RONALD A. CONLON
金额：
$ 7.72万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2023-07-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10531722
关键词：
Applications Grants Archives Arctic Regions Back Biological Process Biomedical Research Cancer Center Cardiovascular Diseases Clinic Communities Cost Control Cryopreservation Cystic Fibrosis Data Development Digestive System Disorders Disease Embryo Ensure Equipment Fertility Foundations Freezing Funding Future Genes Genetic Models Gnotobiotic Immunity Immunology Individual Institution Label Leadership Liquid substance Malignant Neoplasms Modeling Modernization Monitor Mouse Strains Mus National Heart, Lung, and Blood Institute National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institute of Diabetes and Digestive and Kidney Diseases Nerve Degeneration Nitrogen Nucleic Acids Ohio Phase Psoriasis Reproducibility Request for Applications Research Research Institute Research Personnel Resources Retrieval Safety Sampling San Francisco Scientist Secure Security Services Shipping System Temperature Time Transgenic Organisms Translations United States National Institutes of Health University Hospitals Viral base blastocyst cardiometabolism cost cost effective cost efficient cryogenics cystic fibrosis mouse egg fundamental research gene therapy germ free condition innovation interest microbiome microbiome research mouse genetics mouse model operation preimplantation programs public repository repository skeletogenesis sperm cell sperm cryopreservation

项目摘要

Abstract The Case Transgenic and Targeting Facility is the sole transgenic core serving NIH-funded researchers in Northeast Ohio. The core has created and cryopreserved mouse genetic models for over twenty years. These mouse models have been used by local researchers and researchers around the world to study cancer, cystic fibrosis, neurodegeneration, viral immunology, skeletogenesis, cardiovascular disease, and many other diseases and fundamental biological processes. Gene editing increased the number of models that the core has been able to create. Now more than ever, the NIH-funded researchers we serve need these strains backed up, archived for future retrieval, or distributed to their collaborators and other scientists as cryopreserved sperm or embryos. This application requests funding for equipment which will modernize the core's mouse strain cryopreservation, make it more cost-efficient, increase capacity and bring the operation in line with current best practices. The core needs this modernization to meet the demand from local researchers, research centers, and from new institutional research initiatives in translation of gene-directed therapies and in studies of the microbiome.

抽象的案例转基因和靶向设施是由NIH资助的唯一的转基因核心俄亥俄州东北部的研究人员。核心已经创建和冷冻保存的小鼠遗传模型超过二十年。这些鼠标模型已被本地研究人员和全球研究癌症，囊性纤维化，神经退行性，病毒的研究人员免疫学，骨骼生成，心血管疾病以及许多其他疾病和基本疾病生物过程。基因编辑增加了核心能够的模型数量创建。现在，我们服务的NIH资助的研究人员比以往任何时候都更需要这些菌株向上，存档以将来检索或分发给其合作者和其他科学家冷冻保存的精子或胚胎。该申请要求为设备提供资金，这些设备将现代化核心的小鼠应变冷冻保存，使其更具成本效益，增加容量并将操作与当前的最佳实践保持一致。核心需要这个现代化以满足当地研究人员，研究中心和新的需求基因定向疗法翻译的机构研究计划和研究微生物组。